INDICATORS OF THE OXIDANT-ANTIOXIDANT SYSTEM IN THE SYNOVIAL FLUID OF PATIENTS WITH OSTEOARTHRITIS AFTER SARS-CoV2 INFECTION

Borodin S. V., Ostapchenko D. I., Korotkyi O. Н., Dvorshchenko K. O.

CLINICAL AND EXPERIMENTAL MEDICINE

Scentific article

The 2019 coronavirus disease (Coronavirus disease 2019, COVID-19) is a pandemic disease of the 21st century that affects the human population. This is a respiratory disease characterized by the development of serious complications that are dangerous for human life. Often, the development of complications is observed in patients with COVID-19 who were elderly or had other underlying diseases. Among such patients with chronic diseases, patients with pathologies of the musculoskeletal system, particularly osteoarthritis, are an important group. According to clinical research data, an increasing number of cases of joint damage develop in patients after COVID-19, making this condition worthy of further study. Violation of the oxidant-antioxidant balance and the development of oxidative stress play an important role in the pathogenesis of osteoarthritis. The aim of the work was to determine a number of indicators of the oxidant-antioxidant balance in the synovial fluid of patients with osteoarthritis after SARS-CoV2 infection. All study participants were divided into two groups. The first group (n=22) is patients with osteoarthritis of knee joints II-III degree. The second group (n=14) is patients with osteoarthritis of the knee joints II-III degree, who suffered a mild and moderate form of COVID-19 6-9 months ago. In the conducted studies, the concentration of superoxide radical and hydrogen peroxide and the enzymatic activity of superoxide dismutase and catalase in the synovial fluid of patients of all experimental groups were evaluated. The content of the superoxide radical was determined by the formation of XTT formazan. The content of hydrogen peroxide was measured in the sorbitol-xylenol orange system. Superoxide dismutase (SOD) activity was determined by the ability of SOD to compete with nitroblue tetrazolium for superoxide radicals. Catalase activity was measured by the amount of intact hydrogen peroxide in the sample. Processing of research results was carried out using generally accepted methods of variational statistics.It was established that in the synovial fluid of patients with osteoarthritis of the knee joints who contracted COVID-19, the content of superoxide radical increased by 1.6 times and hydrogen peroxide by 1.8 times compared to the group of patients with osteoarthritis. It was found that in the synovial fluid of the joints of patients with osteoarthritis who fell ill with COVID-19, superoxide dismutase activity is reduced by 1.4 times and catalase activity by 1.6 times compared to the group of patients with osteoarthritis. The detected changes indicate a violation of the oxidative-antioxidant balance with excessive formation of reactive oxygen species and a decrease in the activity of antioxidant protection enzymes. This indicates the prevalence of free radical processes and the development of oxidative stress directly in the joint. The results obtained by us indicate that patients with osteoarthritis may have a more severe course of osteoarthritis and the development of complications after infection with COVID-19.

SARS-CoV-2,osteoarthritis,synovial fluid,free radical processes,antioxidant system.

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«Bulletin of problems biology and medicine» Issue 4 (167), 2022 year, 125-130 pages, index UDK 616.91-06:616.72-002

10.29254/2077-4214-2022-4-167-125-130