MORPHOFUNCTIONAL CHARACTERISTICS OF THE CRYPTS STRUCTURAL COMPONENTS OF THE CECUM PERIVULNAR AREA BY USING DESMOSIN SURGICAL THREAD

Kobeniak M. M., Pronina O. M.

MORPHOLOGY

Scentific article

The paper describes the morphological changes in the structural components of the caecal crypts in the periulnar region while suturing the wound defect with desmosin surgical thread. It was determined that rabbits’ cecum, like humans, consists of four membranes: mucous, submucosa, muscular and serous. The mucous membrane included cylindrical epitheliocytes arranged in one layer on its own plate. Under the lamina propria, the mucous membrane’s muscular lamina was visualized, including smooth myositis, which were located in two layers: the inner circular and the outer longitudinal. A large number of crypts and the absence of villi were determined in the composition of the mucous membrane. In the mucosa and submucosa, the elements of the hemomicrocirculatory channel were visualized: arterioles, capillaries, and venules, which in turn provided blood circulation and blood exchange of the wall of the cecum as a whole. The structure of the cecal crypts of animals of the control group included various cell associations: columnar cells with a brush border, goblet cells, single endocrinocytes, columnar epitheliocytes without a brush border, and Paneth cells. Lieberkhun’s glands histotopographically passed through the entire mucous membrane surface, their openings opened into the lumen of the cecum, and their lower parts reached the lamina propria. Thus, it was established that the implementation of alteration processes in the perivulnar region of rabbits’ cecum begins on the 3rd day of the experimental study and is determined in the deep layers of the mucous membrane with the involvement of all its structural components. Connection to the regenerative and compensatory processes of the surface layers of the mucous membrane f rabbits cecum in the area of the wound defect sutured with Desmosin surgical thread occurs from the 7th day of observation, in which the structural components of Lieberkün’s glands are involved. Due to destructive changes in columnar epitheliocytes with a brush border in the crypts of the perivulnar area, digestion and absorption processes are disrupted and possibly stopped from the 3rd to the 7th day of the experimental study. The recovery of the pool of these cellular elements begins from the 14th day of the experiment, and the control indicators reach up to the 30th day. The restoration of the cellular composition of Lieberkün’s glands in the perivulnar region occurred due to the poorly differentiated cells, which tended to increase their average number from 3 to 21 days of the experiment. Recovery of the average number of these cells compared with control indicators occurred on the 30th day of observation.

cecum,crypt,Lieberkün’s glands,Vicryl.

1. Pronina OM, Kobenyak M.M. Makroskopichna kharakterystyka stanu pislyaoperatsiynoho rubtsya tovstoho kyshechnyka pry eksperymentalʹnomu vykorystanni desmosynu. Materialy 2-yi Vseukrayinskoyi naukovo-praktychnoyi konferentsiyi z mizhnarodnoyu uchastyu Teoriya ta praktyka suchasnoyi morfolohiyi. 2018 Zhovten 10-12; Dnipro; 2018. s. 15-17. [in Ukrainian].
2. Wallace SJ, Mioton LM, Havey RM, Muriuki MG, Ko JH. Biomechanical Properties of a Novel Mesh Suture in a Cadaveric Flexor Tendon Repair Model. J Hand Surg Am. 2019;44(3):208-215. DOI: 10.1016/j.jhsa.2018.11. 016. 
3. Onesti MG, Neststi N, Carella S, Scuderi N. Effectiveness of antimicrobialcoated suturesfor the prevention of surgical site infection:a review of the literature. European Review for Medical and Pharmacological Sciences. 2018;22:5729-5739.
4. Pronina OM, Yeroshenko HA, Dalʹnichenko SI, Polovyk OYU. Ulʹtrastruktura tkanyn sechovoho mikhura sobak v ranni stroky pislya eksperymentalʹnoyi tsystotomy z vykorystannyam khirurhichnoyi nytky biofil ta yoho modyfikatsiyi etoniyem. Svit medytsyny ta biolohiyi. 2012;1(32):147-150. [in Ukrainian]. 
5. Bilash SM, Pronina OM, Sydorenko MI. Suchasnyy pohlyad na morfolohiyu tonkoho kyshechnyka pry riznykh patolohichnykh stanakh ta vybori optymalʹnoho shovnoho materialu pry operatyvnykh vtruchannyakh na nʹomu. Visnyk problem biolohiyi i medytsyny. 2019;1(1):20- 24. [in Ukrainian].
6. Berger G, Eviatar E, Kogan T, Landsberg R. The normal uncinate process: histology and clinical relevance. Eur Arch Otorhinolaryngol. 2013;270(3):959-964.
7. Verma P, Verma KG, Khosa R, Kumar S, Basavaraju S, Patwardhan N. Combined use of frontal sinus and nasal septum patterns as an aid in forensics: a digital radiographic study. N Am J Med Sci. 2015;7(2):47-52.
8. Pogorielov M, Kalinkevich O, Deineka V, Garbuzova V, Solodovnik A, Kalinkevich A, et al. Haemostatic chitosan coated gauze: in vitro interaction with human blood and in-vivo effectiveness. Biomaterials research. 2015;19(1):22. DOI: 10.1186/s40824-015-0044-0/.
9. Pronina OM, Kobenyak MM. Porivnyalʹna kharakterystyka morfolohichnykh zmin tovstoho kyshechnyka pry eksperymentalʹnomu vykorystanni kethutu z svynyachoyi syrovyny i kethutu modyfikovanoho L-arhininom dlya ushyvannya yoho ran. Materialy naukovo-praktychnoyi konferentsiyi z mizhnarodnoyu uchastyu Teoriya ta praktyka suchasnoyi morfolohiyi, prysvyachenoyi 100 richchyu Dnipropetrovskoyi shkoly morfolohiv; 2016 Zhovten 5-7; Dnipro; 2016. s. 127-129. [in Ukrainian].
10. Pronina OM, Kobenyak MM. Vplyv khirurhichnykh nytok modyfikovanykh L-arhininom na morfometrychni pokaznyky v tkanynakh operovanoho tovstoho kyshechnyka kroliv v ranni terminy. Zbirnyk materialiv naukovo–praktychnoyi konferentsiyi Prykladni aspekty morfolohiyi; 2016 Zhovten 20-21; Ternopil. Ternopil: DVNZ Ternopilskyy derzhavnyy medychnyy universytet im.I.YA.Horbachevskoho MOZ Ukrayiny; 2016. s. 127-129. [in Ukrainian]. 
11. Gauzit R, Pean Y, Barth X, Mistretta F, Lalaude O, Top Study Team. Epidemiology, management, and prognosis of secondary nonpostoperative peritonitis. French prospective observational multicenter study. Surg Infect. 2009;10(2):119-27.
12. Pronina OМ, Koptev MM, Bilash SМ, Yeroshenko GA. Response of hemomicrocirculatory bed of internal organs on various external factors exposure based on the morphological research data. Svit medytsyny ta biolohiyi. 2018;1(63):153-7. DOI: 10.26.724/2079-8334-2018-1- 63-153-157.

«Bulletin of problems biology and medicine» Issue 4 (167), 2022 year, 296-301 pages, index UDK 616.34–089.8:615.46

10.29254/2077-4214-2022-4-167-296-301