THE USE OF VECTOR – PARAMETRIC FLUORESCENCE POLARIZATION MICROSCOPY OF HISTOLOGICAL SECTIONS OF SPLEEN OF RATS WITH ABDOMINAL SEPSIS IN EXPERIMENT

Solovey Y. M., Polevoy V. P., Solovey M. M.

METHODS AND METHODOLOGIES

Scentific article

. In the experiment, abdominal sepsis of varying severity was modeled on 273 nonlinear white rats: mild, moderate and severe course. Intact animals served as a control group. Internal organs were taken and histological sections of the spleen were prepared at 12, 48 and 78 hours of the experiment. Investigated the structure of the polycrystalline component of biological preparations of histological sections of the spleen differential diagnosis of the severity of septic condition by digital Stokes polarimetric fluorescence microscopy Experimentally tested the optical arrangement of the Stokes polarimetric mapping system with vector-parametric analysis of fluorescence microscopic images of histological sections of the spleen of laboratory rats. An album of vector-parametric polarization maps and distributions of the number of characteristic values of the crystallization parameter at thepoints of digital fluorescence microscopic images of histological sections of the spleen from control group 1 and experimental groups 2 – 4 with different severity of septic pathology was obtained. The statistical reliability of differentiation of vector-parametric maps of fluorescent microscopic images of histological sections of the spleen from control group 1 and experimental groups 2 – 4 with different severity of septic pathology was determined. The most diagnostically sensitive statistical criteria for the differentiation of vector-parametric polarization maps of fluorescence microscopic images of histological sections of the spleen of rats from control group 1 and experimental groups 2 – 4 with different severity of septic pathology were found. The operational characteristics of the diagnostic power of the method of Stokes polarimetric microscopy of fluorescence microscopic images of histological sections of the spleen of rats of control and experimental groups were determined. The balanced accuracy of: differentiation of healthy and septic rats; intergroup differentiation of the severity of the septic process of sick rats; intragroup differentiation of septic rats was established.

abdominal sepsis,spleen,fluorescence microscopy,Stokes vector

1. Desiateryk VI, Polovyi VP. Vnutrishnoocherevynna infektsiia ta perytonit. Chernivtsi: Meduniversytet; 2019. 218 s. [in Ukrainian]. 
2. Polovyi VP, Boiko VV, Sydorchuk RI. Perytonit – odvichna problema nevidkladnoi khirurhii. Chernivtsi: Meduniversytet; 2012. 376 s. [in Ukrainian]. 
3. Boldingh QJ, de Vries FE, Boermeester MA. Abdominal sepsis. Curr Opin Crit Care. 2017 Apr;23(2):159-166.
4. Hecker A, Reichert M, Reuß CJ, Schmoch T, Riedel JG, Schneck E, et al. Intra-abdominal sepsis: new definitions and current clinical standards. Langenbecks Arch Surg. 2019 May;404(3):257-271.
5. Sartelli M, Chichom-Mefire A, Labricciosa FM, Hardcastle T, Abu-Zidan FM, Adesunkanmi AK, et al. The management of intra-abdominal infections from a global perspective: 2017 WSES guidelines for management of intra-abdominal infections. World J Emerg Surg. 2017;12:29.
6. Blot S, Antonelli M, Arvaniti K, Blot K, Creagh-Brown B, de Lange D, et al. Epidemiology of intra-abdominal infection and sepsis in critically ill patients: “AbSeS”, a multinational observational cohort study and ESICM Trials Group Project. Intensive Care Med. 2019 Dec;45(12):1703-1717.
7. Clements TW, Tolonen M, Ball CG, Kirkpatrick AW. Secondary Peritonitis and Intra-Abdominal Sepsis: An Increasingly Global Disease in Search of Better Systemic Therapies. Scand J Surg. 2021 Jun;110(2):139-149.
8. Railianu S, Solovey Yu, Polovyi V, Dubolazov A, Ushenko Yu, Soltys, I, et al. Vector-parametric structure of polarization images of networks of biological crystals for differential diagnosis of inflammatory processes. Proc. SPIE Applications of Digital Image Processing XLIII. 2020;115102M:1-9.
9. Solovei YuM, Polovyi VP, Solovei MM, Solovei VM, vynakhidnyky; Solovei YuM, Polovyi VP, Solovei MM, Solovei VM, patentovlasnyky. Sposib vektor-parametrychno fliurystsentno poliaryzatsiinoho vyznachennia stupenia vazhkosti abdominalnoho sepsysu. Patent Ukrainy na korysnu model № 150796. 2022 Kvit 16. [in Ukrainian].

«Bulletin of problems biology and medicine» Issue 4 (167), 2022 year, 269-280 pages, index UDK 617.55-002:616.94]-07:612.411.086.2

10.29254/2077-4214-2022-4-167-269-280