ОЦЕНКА ДИФФЕРЕНЦИАЛЬНОЙ ЭФФЕКТИВНОСТИ МЕТОДОВ ОКРАСКИ ООЦИСТ КРИПТОСПОРИДИЙ

Яковенко Д. В., Похил С. И., Тимченко Е. Н., Чигиринская Н. А., Костыря И. А.

МЕТОДЫ И МЕТОДИКИ

Научная статья.

На основании результатов испытания шести наиболее употребляемых в мировой медицинской практике методов окрашивания ооцист криптоспоридий установлено, что аурамин-родаминовий метод (ARM) характеризуется относительно более высокой дифференциальной эффективностью. При проведении люминисцентной микроскопии окрашенных ARM мазков суспензий ооцист на удовлетворительном уровне визуализируются все дифференциальные признаки ооцист, в том числе, присущая им внутренняя структурированность.

ооцисты криптоспоридий, методы окраски, дифференциальная эффективность

1. Putignani L, Menichella D. Global Distribution, Public Health and Clinical Impact of the Protozoan Pathogen Cryptosporidium. Interdiscip Perspect Infect Dis. 2010;2010:39. Available from: PMID: 20706669. DOI: 10.1155/2010/753512
2. Peletz R, Mahin T, Elliott M, Montgomery M, Clasen T. Preventing cryptosporidiosis: the need for safe drinking water. Bull World Health Organ. 2013 Apr 1;91(4):238-8A. PMID: 23599543. DOI: 10.2471/BLT.13.119990
3. Shrivastava AK, Kumar S, Smith WA, Sahu PS. Revisiting the global problem of cryptosporidiosis and recommendations. Trop Parasitol. 2017 Jan-Jun;7(1):8-17. DOI: 10.4103/2229-5070.202290
4. Cryptosporidiosis (Cryptosporidium spp.) 2012 Case Definition. CDC. National Notifiable Diseases Surveillance System [Internet]. Available from: https://wwwn.cdc.gov/nndss/ conditions/cryptosporidiosis/case-definition/2012/
5. Nakaz MOZ Ukrayiny vid 28.12.2015 r. № 905 «Pro zatverdzhennya kryteriyiv, za yakymy vyznachayut’sya vypadky infektsiynykh ta parazytarnykh zakhvoryuvan’, yaki pidlyahayut’ reyestratsiyi». Dostupno: http://old.moz.gov.ua/ua/portal/dn_20151228_0905.html [in Ukrainian].
6. Chalmers RM. Cryptosporidium: from laboratory diagnosis to surveillance and outbreaks. Parasite. 2008;15(3):372-8. PMID: 18814709. DOI: 10.1051/parasite/2008153372 Available from: http://dx.doi.org/10.1051/parasite/2008153372
7. Chalmers RM, Katzer F. Looking for Cryptosporidium: the application of advances in detection and diagnosis. Trends Parasitol. 2013 May;29(5):237-51. DOI: 10.1016/j.pt.2013.03.001
8. Ghazy AA, Abdel-Shafy S, Shaapan RM. Cryptosporidiosis in animals and man: 2. Diagnosis. Asian J Epidemiol. 2015;8(4):84-103. DOI: 10.3923/ aje.2015.84.103
9. Vohra P, Sharma M, Chaudary U. A comprehensive review of diagnostic techniques for detection of Cryptosporidium parvum in stool samples. J Pharm. 2012;2(5):15-26.
10. Basic laboratory methods in medical parasitology. Library catalog of publications WHO. World Health Organization. Geneva, 1994. 131 р. Available from: www.who.int/iris/bitstream/10665/141252/1/5225032508.pdf
11. Garcia LS, Campbell JL, Fritsche TR, Hummert B, Johnston SP, Rachfold FW, et al. Procedures for the Recovery and Identification of Parasites from Intestinal Tract; Approved Guideline-Second Edition. CLSI document M28-A2. 2005;25(16):111.
12. Cryptosporidiosis. Laboratory Diagnosis [Internet]. CDC. DPDx (updated 2016 March 1) Available from: https://www.cdc.gov/dpdx/ cryptosporidiosis/dx.html
13. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. Cryptosporidiosis. Chapter 2.9.4. World Orgаnisation for Animal Health (OIE). 2008;2:1192-215. Available from: www.oie.int/doc/ged/D7709.pdf
14. Elliot A, Morgan UM, Thompson RC. Improved staining method for detecting Cryptosporidium oocysts in stools using malachite green. J. Gen. Appl. Microbiol. 1999;45(3):139-42. Available from: https://doi.org/10.2323/jgam.45.139
15. Modified Kinyoun's Acid-Fast Stain (cold) Protocol [Internet]. Medical Chemical Corporation. 2017. 6 p. Available from: www.med-chem.com/ pages/.../modified_kinyoun _cold.pdf
16. Kageruruka P, J. Brandt RA, Taelman H, Jonas C. Modified Köster staining method for the diagnosis of Cryptosporidiosis. Ann Soc Belg Med Trop. 1984;64(2):171-5. PMID: 6207782
17. Modified auramine-rhodamine stain kit [Internet]. Dalynn Biologicals Inc. 2014. 2 p. Available from: http://www.dalynn.com/dyn/ck_assets/ files/tech/SA93.pdf
18. ОАО «LOMO». Mikroskop dlya klinicheskoy laboratornoy diagnostiki MIKMED-2. Rukovodstvo po ekspluatatsii YU-33.22.926-03 RE. OAO «LOMO». 2006. 45 s [in Russian].
19. Xiao L. Molecular epidemiology of cryptosporidiosis: an update. Exp Parasitol. 2010;124(1):80-9. PMID: 19358845. DOI: 10.1016/j. exppara.2009.03.018
20. Ryan U, Fayer R, Xiao L. Cryptosporidium species in humans and animals: current understanding and research needs. Parasitol. 2014;141:166785.
21. Lakin GF. Biometriya. Moskva: VS. 1990. 352 s. [in Russian].
22. Potters I, Esbroeck MV. Negative staining technique of heine for the detection of Cryptosporidium spp.: a fast and simple screening technique. J Parasitol. 2010;4:1-4. DOI: 10.2174/1874421401004010001
23. Rekha KMH, Puttalakshmamma GC, D’Souza EP. Comparison of different diagnostic techniques for the detection of cryptosporidiosis in bovines. Vet World. 2016;9(2):211-5. PMID: 27051211. DOI: 10.14202/vetworld.2016.211-215
24. Ignatius R, Klemm T, Zander S, Gahutu JB, Kimmig P, Mockenhaupt FP, et al. Highly specific detection of Cryptosporidium spp. oocysts in human stool samples by undemanding and inexpensive phase contrast microscopy. Parasitol Res. 2016;115(3):1229-34. PMID: 26646397. DOI: 10.1007/s00436-015-4859-3
25. McHardy IH, Wu M, Shimizu-Cohen R, Couturier MR, Humphries RM. Detection of intestinal protozoa in the clinical laboratory. J Clin Microbiol. 2014;52(3):712-20. PMID: 24197877. DOI: 10.1128/JCM.02877-13
26. Tortora GT, Malowitz R, Mendelsohn B, Spitzer ED. Rhodamine-auramine O versus Kinyoun-carbolfuchsin acid-fast stains for detection of Cryptosporidium oocysts. Clin Lab Sci. 1992;5(6):568-9. PMID: 10150976
27. Petry F. Structural Analysis of Cryptosporidium parvum. Microsc Microanal. 2004;10(5):586-601. PMID: 15525433. DOI: 10.1017/ S1431927604040929
28. Lager DJ, Landas SK. Correlative Light and Scanning Electron Microscopy of Intestinal Giardiasis, Cryptosporidiosis, and Spirochetosis. Ultrastruct Pathol. 1991;15(6):585-91. PMID: 1799024
29. Thompson RC, Olson ME, Zhu G, Enomoto S, Abrahamsen MS, Hijjawi NS. Cryptosporidium and Cryptosporidiosis. Adv Parasitol. 2005;59:77158. PMID: 16182865. DOI: 10.1016/S0065-308X(05)59002-X
30. Kang G, Mathan MM. A comparison of five staining methods for detection of Cryptosporidium oocysts in faecal specimens from the field. Indian. Indian J Med Res. 1996;103:264-6. PMID: 8707362

«Вестник проблем биологии и медицины» Выпуск 4 Том 1 (146), 2018 год, 198-203 страницы, код УДК 619:616.993.1

10.29254/2077-4214-2018-4-1-146-198-203