The State of Prooxidant-Antioxidant System in Chronic Experimental Gastroenterocolitis
About the author:
Tkachenko A. S., Gopkalov V. H.
Heading:
CLINICAL AND EXPERIMENTAL MEDICINE
Type of article:
Scentific article
Annotation:
An increase in the number of gastroenterological patients has been found in recent years with the en- hanced frequency rate of inflammatory bowel disease cases. It has been established that features of contemporary diet, including excessive consumption of food additives, might be considered as a possible cause of inflammatory bowel disease. Our attention is paid to food additive E407, also known as carrageenan. Carrageenans are sulfated polysaccharides extracted from red seaweeds and used as thickeners and stabilizers in food industry. It has been known that long-term intake of carrageenan causes the development of chronic gastroenterocolitis (GEC) in rats. This model of chronic carrageenan-induced GEC of moderate severity allows studying the features of carrageenan- induced inflammation of the gastrointestinal tract, such as the status of prooxidant-antioxidant system that is still not investigated. The aim of the investigation was to study the peculiarities of prooxidant-antioxidant system functioning in rats with chronic carrageenan-induced GEC. The female Wistar rats were used for the experiment. Chronic carrageenan-induced gastroenterocolitis was reproduced by the free access of animals to 1 % solution of carrageenan in drinking water. Laboratory animals were divided into 3 groups. Group № 1 consisted of experimental animals, who consumed food additive carrageenan during 2 weeks. Group № 2 consisted of experimental animals, who consumed food additive carrageenan dur- ing one month and group № 3 consisted of intact animals. The development of GEC was proved morphologically and biochemically. Manipulations with animals were carried out in accordance with the provisions of the European Convention for the Protection of Vertebrate Animals used for Experimental and other Scientific Purposes (Stras- bourg, 1986). The concentration of TBA-active products – malonic dialdehyde (MDA) and dienic conjugates (DC), total antioxidant activity (TAA), products of protein oxidative modification (POM), activity of superoxide dismutase (SOD) and catalase in the blood serum were determined by spectrophotometric methods. It was found that rats the level of TBA-active compounds and concentration of carbonylated proteins in blood serum of rats were significantly increased after 2 weeks of carrageenan intake compared to control group. After one month of carrageenan consumptions their levels were lower compared to the first group, but noticeably higher compared to laboratory animals of the control group. Such changes, indicated the activation of lipid peroxidation and development of carbonyl stress in chronic carrageenan-induced GEC, were more profound after 2 weeks of carrageenan intake. The level of TAA in blood serum of rats was decreased only after one month of carrageenan intake compared to control group. The activity of both SOD and catalase were increased in animals of the first and the second groups. The increased activity of SOD and catalase in serum of rats from the first group combined with unchanged serum TAA showed the development of compensatory responses to activation of free radical processes, which, however, were not strongly developed. In experimental animals of the second group a significant suppression of serum anti- oxidant activity was observed that manifested by slightly increased activity of SOD, catalase and decreased serum TAA. It indicated the development of oxidative stress in animals with chronic carrageenan-induced GEC.
Tags:
gastroenterocolitis, carrageenan, lipid peroxidation, oxidative stress, carbonyl stress, total antioxi- dant activity.
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Publication of the article:
«Bulletin of problems biology and medicine» Issue 1 (106), 2014 year, 194-198 pages, index UDK 577. 12:616. 33/. 34-002. 2:57. 084