FEATURES OF MORPHOLOGICAL CHANGES OF FILAMENTOUS PAPILLAE OF THE TONGUE AT EXPERIMENTAL DIABETES MELLITUS

Atamanchuk O. V.

MORPHOLOGY

Scentific article

The mucous membrane of the tongue is considered one of the most important structures of the organism that performs multifaceted functions throughout life. Clinical changes of the mucous membrane of the tongue are determined by morphofunctional features and due to its localization in the initial digestive tract systems. Due to the general neuroreflex regulation is special effects on the tissues of the tongue create chronic diseases, including diabetes mellitus. The neurotrophic component in diabetes plays an important role in pathogenesis of destructive and inflammatory lesions of the tongue, but the mechanism of these lesions and morphological manifestations are insufficiently studied. Therefore, the aim of our study was to study morpho-functional changes in the filamentous papillae of the tongue of adult rats at experimental streptozotocin diabetes mellitus. Twenty adult white male rats of the line were used for the study Wistar (body weight 180-200 g), which were equally divided into 2 groups: experimental, control. Experimental group (10 rats) streptozotocin “SIGMA” (USA) was administered once intraperitoneally, which was diluted in 0.1 M citrate buffer with a pH of 4.5 at a rate of 6 mg per 100 g body weight. Control group (10 rats) in an equivalent dose once 0.1 M citrate buffer pH 4.5 was administered intraperitoneally. Capture material was carried out after 4 and 8 weeks from the start of modeling streptozotocin diabetes mellitus after previous euthanasia under thiopental anesthesia. Histological method of research was used (hematoxylin and eosin staining) and scanning electron microscopy. Microphotographs were used for morphometric studies. Morphometry was performed using NIH USA “Image J” and “Bio” programs Vision 4.01 ”in manual mode with magnification. Computer data processing was performed using a statistical package STATISTICA (StatSoft, Inc. (2010), STATISTICA (data analysis software system), version 10. Statistical changes were considered significant when achieved the level of statistical significance was p<0.05. In 4 weeks from the beginning of experimental modeling streptozotocin diabetes mellitus, compared with the control group, there is malnutrition of the papillae, resulting in smoothing their microrelief, the diameter and height of filamentous papillae decreases, the distance increases between them. Keratohyalin masses accumulate on the tops of individual papillae bizarre shape. In 8 weeks from the beginning of modeling of experimental diabetes mellitus diabetic changes in the mucous membrane of the tongue are particularly pronounced. On the surface of the mucous membrane of the tongue is a large number of lamellar structures and keratin conglomerates. These structures are especially clear can be traced on the tops of filamentous and rolled papillae. On some areas the entire surface of the papillae is covered with small globular structures that makes them similar to mulberry fruit. On preparations of a mucous membrane tongue we often observed whole complexes of adhered microorganisms. It should be noted that 8 weeks after the start of the experiment there is a colorful histological picture, which is characterized a combination of atrophic and hyperplastic processes in the mucous membrane of the tongue.Conclusions. Streptozotocin diabetes leads to destructive changes in the mucous membrane of the tongue, accompanied by phenomena acanthosis and hyperkeratosis and adhesion of pathogenic microflora on its surface. There is malnutrition of filamentous papillae, resulting in smoothing their microrelief, decreases in diameter and height, increases the distance between them which is confirmed by a decrease in their density by 1,05-2 times.

tongue, mucous membrane of the tongue, filamentous papillae, streptozotocin diabetes mellitus.

1. Semenova AK. Morfofunktsionalna kharakterystyka remodeliuvannia slyzovoi obolonky spynky yazyka shchuriv pislia vplyvu metakrylatu. Visnyk problem biolohiyi i medytsyny. 2018;146(1):226-28. [in Ukrainian].
2. Ieroshenko HA, Semenova AK, Hasiuk NV. Poshyrenist ta struktura zakhvoriuvan yazyka u patsiientiv molodoho viku. World of medicine and biology. 2013;40(3):14-6. [in Ukrainian].
3. Chiang CP, Chang JY, Wang YP, Wu YH, Wu YC, Sun A. Atrophic glossitis: Etiology, serum autoantibodies, anemia, hematinic deficiencies, hyperhomocysteinemia, and management. J Formos Med Assoc. 2020;119(4):774-780. DOI: 10.1016/j.jfma.2019.04.015.
4. Sultan R, Pokotilo PB, Gnidik YuV. Perebudova gemomikrotsirkulyatornogo rusla yazika schura v dinamitsi perebigu eksperimentalnogo tsukrovogo diabetu. Visnyk problem biolohiyi i medytsyny. 2016;2(1):195-199. [in Ukrainian].
5. Yeroshenko HA, Semenova AK, Nebesna ZM. Histofiziolohiya slyzovoyi obolonky spynky yazyka shchuriv pislya diyi metakrylatu. Visnyk problem biolohiyi i medytsyny. 2018;147(2):277-81. [in Ukrainian].
6. Somova KT. Yazyik i zdorove rebyonka. Pediatrics. 2011;2:98-100. [in Ukrainian].
7. Zhurakivska OYa, Koshkin OYe, Tkachuk YL, Rudyak OM, Knyazevych-Chorna TV. Age characteristics of morphogenesis of diabetic myopathies. Problemy endokrynnoyi patolohiyi. 2020;4:115-123. DOI: 10.21856/j-PEP.2020.4.15. [in Ukrainian].
8. Dando R, Pereira E, Kurian M. A permeability barrier surrounds taste buds in lingual epithelia and blood vessels tongue. Am. J. Physiol. Cell Physiol. 2015;308(1):21-32.
9. Klemm PM, Schembri A. Bacterial adhesins: function and structure. Int. J. Med. Microbiol. 2010;290:27-35.
10. Zadik Y, Burnstein S, Derazne E. Colonization of Candida: prevalence among tongue-pierced and non-pierced immunocompetent adults. Oral Diseases. 2010;16(2):172-175.
11. Okubo Y, Tsukadaira A, Takashi S. Chemotaxis of human CD4+ eosinophils. Int. Arch. Allergy Immunol. 2011;125(1):19-21.
12. Berg AK. Нaracterization of organotypic keratinocyte cultures on deepithelialized bovine tongue mucosa. Histol. Histopathol. 2012;1:221- 230.
13. Stähler F, Brennick MJ, Delikatny J. Tongue Fat Infiltration in Obese Versus Lean Zucker Rats. Sleep. 2014;37(6):1095-1102.
14. Ruffoli R, Giambelluca MA, Scavuzzo MC. Athrofia glossoalgia: a morphofunctional investigation in children. Oral Diseases. 2015;21(3):170- 174.
15. Iordanishvili AK, Filippova EV, Libih DA. Kliniko-funkcional’noe sostoyanie slizistoj obolochki polosti rta i yazyka u lyudej starshih vozrastnyh grupp. Institut stomatologii. 2012;4:80-81. [in Russian].

«Bulletin of problems biology and medicine» Issue 1 (163), 2022 year, 254-258 pages, index UDK 611.313:611.1:611.018.83:616.379 – 008.64

10.29254/2077-4214-2022-1-163-254-258