IMMUNOLOGICAL REACTIVITY OF THE ORGANISM IN GENERALIZED PERIODONTITIS ON CHRONIC ATROPHIC GASTRITIS BACKGROUND

Bedeniuk O. S., Korda M. M.

CLINICAL AND EXPERIMENTAL MEDICINE

Scentific article

Inflammatory periodontal diseases are one of the important problems of modern dentistry, which is caused by a wide spread among the population and a high incidence of relapses. The aim of the study was to investigate the changes in humoral immune system and cytokine profile in lipopolysaccharide inflammation of periodontal tissue on the chronic gastritis background. The development of atrophic gastritis was confirmed by histological studies. In the experiment, 40 outbred male rats weighing 150-180 g were used, which were kept on a standard vivarium diet. Periodontitis was initiated in white rats by the injection of E. Coli lipopolysaccharide (40 μl, 1 mg/ml) into gingival tissues. For the modeling of chronic gastritis 2% sodium salicylate was administered intragastrically for 6 weeks. Results were expressed as an average + SEM of 10 experiments. Changes in p<0.05 were considered statistically significant. The statistical analysis was performed using standard statistical programs and Student's t-test. Lipopolysaccharide periodontal inflammation was accompanied by the increase in serum of immunoglobulins A, M and G content, circulating immune complexes, as well as pro-inflammatory cytokines TNF-α, IL-1β and by the decrease of anti-inflammatory cytokines IL-4 and IL-10 levels. In animals with periodontitis that developed on the chronic gastritis background, changes in humoral immunity parameters and imbalance between pro- and anti-inflammatory cytokines were expressed more markedly than in rats with periodontitis without gastritis. In the pathogenesis of lipopolysaccharide inflammation of the periodontal tissues an important role belongs to the violation of the humoral level of the immune system and the cytokine profile of the body. Violation of the humoral level of the immune system, together with the imbalance of the cytokine profile, is an important link in the pathobiochemical mechanisms of the development of periodontitis in the context of chronic atrophic gastritis. It has been concluded that chronic gastritis significantly increases the negative effect of lipopolysaccharide inflammation of periodontal tissues on the immune system.

lipopolysaccharide, periodontitis, chronic gastritis, immunoglobulins, cytokines

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«Bulletin of problems biology and medicine» Issue 1 Part 2 (143), 2018 year, 85-88 pages, index UDK 616.314.17-008.1-06:616.33-002.2-06:612.017.1

10.29254/2077-4214-2018-1-2-143-85-88