THE STATE OF NITROGEN (II) OXIDE SYSTEM IN RATS WITH MODELED «PASSIVE TOBACCO SMOKING» COMBINED WITH PROLONGED ADMINISTRATION OF MONOSODIUM GLUTAMATE IN THE SEX AND AGE ASPECTS

Rutska A. V., Krynytska I. Y.

CLINICAL AND EXPERIMENTAL MEDICINE

Scentific article

Research purpose. To investigate the functional state of the nitrogen (II) oxide system in rats in case of «passive tobacco smoking» combined with prolonged administration of monosodium glutamate in the sex and age aspects. Object and research methods. Experiments were performed on 128 white mature and immature rats of both sexes, which were kept on a standard vivarium diet. Each group of animals was divided into four subgroups: I – control (n = 8); ІІ – rats with modeled «passive tobacco smoking» (n = 8); ІІІ – rats, which were injected with monosodium glutamate (n = 8); IV – rats with modeled «passive tobacco smoking» combined with the monosodium glutamate injection (n = 8). The total activity of NO-synthase (NOS) in lung tissue was determined colorimetrically by the number of formed nitrates and nitrites in the incubation medium. The number of formed nitrates and nitrites (NO x ) in blood serum and lung tissue was determined by the Griess method. Research results and their discussion. In case of «passive tobacco smoking», we have established a marked increase in the content of NO x in both serum and in the lung tissue homogenate of mature animals of both sexes. The total activity of NOS has increased by 39.1% (p<0.01) in males and by 50.6% (p<0.001) in females. After prolonged administration of monosodium glutamate, we have established a more pronounced increase in the NO x content of both serum and lung tissue in male rats (2.3 and 2.7 times respectively). Female rats also showed NOS activation and increased of NO x content vs control group, but the changes were less pronounced compared with indices of “passive tobacco smoking” animals. In animals with modeled «passive tobacco smoking» combined with the monosodium glutamate injection, a significant increase in the content of NO x in both serum and in the lung tissue homogenate was established. The total activity of NOS synthase has increased by 24.6% (p <0.02) in males and by 69.1% (p<0.001) in females. In case of «passive tobacco smoking», we have established a significant decrease in the content of NO x in both serum and in the lung tissue homogenate of immature animals of both sexes vs control group. The total activity of NOS has decreased by 22.9% (p<0.05) in males and by 33.3% (p<0.01) in females. After prolonged administration of monosodium glutamate to immature rats, we have established a pronounced increase in NO x content in both serum and in the homogenate of lung tissues in males (2.1 and 2.4 times respectively) and females (2.0 and 2.6 times, respectively) compared to the control group. The total activity of NO synthase in the lung tissue homogenate increased by 45.9% (p <0.002) in males and by 39.1% (p <0.01) in females. In immature rats with modeled «passive tobacco smoking» combined with the monosodium glutamate injection, no significant changes in the nitrogen (II) oxide system in both serum and in the lung tissue homogenate vs the control group were established. Conclusions. An intensification of nitroxydergic processes in both the lung tissue homogenate and blood serum of the both sexes mature rats with modeled «passive tobacco smoking» has been established, which may indicate a compensatory enhancement of nitrogen (II) oxide synthesis. In the sexual comparison of changes in NO-system, their reliable prevalence in female-rats was revealed. In case of «passive tobacco smoking» combined with the introduction of monosodium glutamate, an intensification of nitroxydergic processes was also observed, but the changes were not more pronounced than in case of «passive tobacco smoking». In immature rats of both sexes, in case of «passive tobacco smoking», reduction of nitroxydergic processes was observed both in the lung tissue homogenate and serum, which may indicate the depletion of adaptive mechanisms of regulation of the nitrogen (II) oxide formation and endothelial dysfunction. In case of «passive tobacco smoking» combined with the monosodium glutamate injection an intensification of nitroxydergic processes has been established.

tobacco smokе, sodium glutamate, nitrogen (II) oxide

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«Bulletin of problems biology and medicine» Issue 1 Part 2 (143), 2018 year, 189-194 pages, index UDK 612.015-06:613.84:612.013:616-099:547.466.64]-092.9

10.29254/2077-4214-2018-1-2-143-189-194