ANALYSIS OF THE IMMUNOCOMPETENT CELL COMPOSITION OF TERMINAL BRONCHILES DURING CHRONIC OBSTRUCTIVE PULMONARY DISEASES
About the author:
Kuyun L. O.
Heading:
MORPHOLOGY
Type of article:
Scentific article
Annotation:
Objective: analyse the immunocompentent cell composition of terminal bronchioles in patients with chronic obstructive pulmonary diseases (COPD). Methods and materials. Histological samples originated from 10 patients who had been operated on in Kiev municipal hospital №17 on account of bullous emphysema. The research included ten patients with COPD, whereas the control group consisted of 6 patients who did not have COPD. Lung tissue samples were taken from both groups and submitted for sector-based cross section analysis. Lung tissue was placed into 10% neutral-buffered formalin for 18-24 hours. After that, tissue pieces were extracted. 3 slices 3-5 mm long were obtained by cross section of the lung tissue. The samples were dehydrated using ethanol solutions of different concentrations and dipped in paraffin wax. Serial cross-section samples 4 mkm in width were produced using a rotatary microtome (Leica RM 2125 RT, Germany). The samples were placed on slides and colored using hematoxylin and eosinum (Merck, Germany) and on the SuperFrost Plus adhesive slides for further immunohistological research. Immunohistochemical (IHC) studies were performed by using primary monoclonal antibodies of mouse to Ki-67 (proliferation index), CD3, CD4, CD8, CD20, CD45, CD45R0 and CD68 (DAKO, Denmark) and EnVision + System-HRP imaging systems (DAB). The data obtained in the experiment was statistically processed using “Minitab 16” statistical software. Results and their discussion. The results of the study have shown that in COPD there was a significant increased (p <0.001) of infiltration of the terminal bronchioles compared to control by common T-lymphocytes (CD3 +), T-helpers (CD4 +) and cytotoxic T-lymphocytes (CD8 +) content. At the local level the immune response during COPD does not depend on migration of macrophages (CD68 +), B-lymphocytes (CD20 +), CD45 + cells, CD45RO + cells and Ki67 + cells into the inflammation area. This was found to be true for both the COPD and the control groups. At the systemic level, peripheral blood of COPD patients during exacerbation contained increased levels of T-lymphocytes (CD8+). At the same time, the level of cytotoxic T-lymphocytes (CD8+) type I increased, whereas level of cytotoxic T-lymphocytes (CD8+) type II decreased during the stable stage of the disease. A different author has noted that CD4+ и CD8+ levels in the peripheral blood decreased, which was justified by their migration into the areas of inflammation in the bronchi. Another research conducted by Eapen M.S. et al. (2017) has shown results somewhat similar to ours. Notably, the levels of T-lymphocytes (CD8+) in COPD patients were statistically higher than in the control group (P < 0.01). However, concentrations of macrophages and CD4+ T-lymphocytes did not differ statistically. Significance of the research. The importance of the application of this data based both on quantitative and qualitative characteristics of the local response of CD4+ and CD8+ T - lymphocyte subpopulations is undeniable. These subpopulations determine the course that the disease will take in COPD patients and aid in developing the most efficient and specific immune therapy. The author is convinced that individual approach to treatment of COPD patients is vital.
Tags:
COPD, cellular infiltration of bronchioles, immunohistochemistry, local immunity
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Publication of the article:
«Bulletin of problems biology and medicine» Issue 1 Part 2 (143), 2018 year, 307-311 pages, index UDK 616.24-007.271-097+616.235-097