THE DEGREE OF ACCUMULATION OF CADMIUM AND ZINC IN THE LIVER OF MALE RATS AND THEIR INFLUENCE ON TRANSAMINAZ ACTIVITY

Shamelashvili K. L., Shatorna V. F., Toрka E. G, Kosse V. A., Lyulko I. V., Alekseenko Z. K., Filippov Y. O.

BIOLOGY

Scentific article

Cadmium poisoning in humans occurs through inhalation of cigarette smoke and ingestion of contaminated food and water. The toxic effect of cadmium affects the liver, kidneys, lungs, etc. Zinc-containing compounds reduce the absorption and accumulation of cadmium and mitigate some of its toxic effects. Purpose of the work. Determine the degree of accumulation of salts of cadmium and zinc succinate in the liver of Wistar rats. Study of the effect of cadmium and zinc succinate salts on the activity of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in the blood plasma of rats. Experimental studies were carried out on male Wistar rats. To simulate the influence and toxic effect of exposure to cadmium chloride, we injected a solution of cadmium chloride (at a dose of 2.0 mg/kg) daily per os throughout 13 and 19 days. In the second experimental group, a combined administration of cadmium chloride (at a dose of 2.0 mg/kg) and zinc succinate (at a dose of 5.0 mg/kg) was carried out. The slaughter was carried out on the 13th and 19th days of the introduction of the compounds under study. Quantitative measurement of the content of metals in the samples was carried out on an EMAS-200 CCD atomic emission spectrometer. The enzymatic activity of AST and ALT was determined according to the method of determination of these enzymes by Reitman-Frankel. The assessment of the reliability of statistical studies was carried out using the Student’s t-test. Zinc succinate compounds are able to reduce the content of cadmium in the liver of male rats when administered together for 19 days by 10%. While cadmium does not significantly affect the accumulation of zinc in the liver. According to the data obtained, the accumulation of cadmium in the liver leads to an increase in the activity of enzymes such as AST and ALT, which are responsible for the regulation of metabolite flows between organs. ALT pumps up glucose, and AST compensates for the increase in ammonia. At the same time, the combined administration of cadmium chloride and zinc succinate reduces the toxic effect of cadmium to a control level.

cadmium chloride, zinc succinate, , liver, ALT, AST.

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«Bulletin of problems biology and medicine» Issue 3 (161), 2021 year, 106-110 pages, index UDK 599.323.4:591.436:591.3:546.48:616-092.9

10.29254/2077-4214-2021-3-161-106-110