BIOLOGICAL EFFECTS OF 15-CROWN-5 WHEN ACTIONG ON RATS CELLULAR MEMBRANES STATE AT SUBACUTE EXPERIMENT

Kratenko R. I.

BIOLOGY

Scentific article

The paper represents the experimental results of 15-crown-5 biological action on phospholipids composition of erythrocytes and hepatocytes membranes, as well as, on the state of membrane-bound neurotransmitter receptors of brain synaptosomes. Objective. Investigation of 15-crown-5 influence upon cellular biological membrane state of rat organism at sub-acute experiment. Object and methods. The experiment was performed with the usage of three-months-old Vistar line white male rats. The experimental animals were administered with 15-crown-5 (water solution) within one month (daily) per orally in 1/100 LD50 (13.5 mkg/kg). The control group of animals was given water. Lipid extraction was performed by the Cates method. Evaporation of micro-thin-layer chromatography was used as a method for separation of individual phospholipids factions. Determination of membrane-bound receptors complexes activity was investigated in rat brain synaptosomes. The experiment used 3Н-WB4101, 3Н-dihydroalprenolole, 3Н-serotonin and 3Н-spiperone, as selective ligands for α1-, β1- adreno-, 5-НТ1-, 5-НТ2-serotonin receptors, correspondently. The characteristics of the receptors functional state implied the calculation of dissociation constant (Kd), and binding sites maximal quantity (Bmax). Results. The action of 15-crown-5 resulted in alterations of membrane phospholipids faction composition. The compound increased percentage contents of phosphatidyl choline and lisophosphatidyl choline in erythrocytes, decreasing sphyngomyelin and phosphatidyl inositole percentage. In the liver, the chemical increased phosphatidyl choline, lisophosphatidyl ethanole amine and lisophosphatidyl choline percentage. The increase in phospholipids lysoforms percentage verifies lipid peroxidation of experimental animals organism membranes at the influence of crown-ethers biotransphormation products. 15-crown-5 unidirectionally influenced the receptory link of cellular signal discrimination, decreasing dissociation constants, and increasing or decreasing binding sites maximal quantity of α-, β1-, 5-НТ1 and 5-НТ2-receptors of rat neocortex synaptosomes. Unidirectional changes of receptory apparatus prove non-specific modulatory influence of 15-crown-5, first of all, on phospholipid microsurrounding, as a part of receptory complexes.

15-crown-5, phospholipids, adrenoreceptors, serotonin receptors

• Balli M.B. Tekuchest’ membrany v biologii: koncepcii membrannyh struktur / M.B. Balli, B. Besterling, Dzh.D. Brejlsford. – Kiev: Naukova dumka, 1989. – 312 s.
• Baraboj V.A. Okislitel’no-antioksidantnyj gomeostaz v norme i patologii / V.A. Baraboj, D.A. Sutkovoj. – K.: Naukova dumka, 1997. – 420 s.
• Djatlovickaja Je.V. Lipidy kak biojeffektory / Je.V. Djatlovickaja, V.V. Bezuglov // Biohimija. – 1998. – T. 63. – Vyp. 1. – S. 3-5.
• Zacepina D.N. Membrannye potencialy i adaptacionnye sposobnosti limfocitov i jeritrocitov v razlichnyh sostojanijah zhiznedejatel’nosti mlekopitajushhih / D.N. Zacepina, G.V. Vengrus, N.N. Gorjunov // Biofizika. – 1993. – T. 38. – № 6. – S. 1098- 1103.
• Kejts M. Tehnika lipidologii / M. Kejts. – M.: Mir, 1975. – 322 s.
• Kratenko R.І. Bіologіchnі efekti 15-kraun-5 pri dіу na aktivnіst’ sistemi mіkrosomal’nogo okislennja ta vіl’no-radikal’nі procesi u pіd gostromu eksperimentі / R.І. Kratenko // Vіsnik problem bіologіі і medicini. – 2017. – Vip. 3, t. 1. – S. 82-88.
• Lang S.M. Laboratornaja krysa / S.M. Lang, R.P. Uilson // Laboratornye zhivotnye. – 1993. – № 2. – S. 101-110.
• Mak-Mjurej U. Obmen veshhestv u cheloveka / U. Mak-Mjurej. – M.: Mir, 1980. – 340 s.
• Findlej Dzh.B. Biologicheskie membrany / Dzh.B. Findlej, U. Jevans. – M.: Mir, 1990. – 424 s.
• Brockhuse R.M. Phospholipids structure of erythrocytes and hepatocytes / R.M. Brockhuse // Clin. Biochem. – 1974. – № 3. – P. 157-158.
• Bylund D.B. Beta-adrenergic receptor binding in membrane preparations from mammalian brain / D.B. Bylund, S.H. Snyder // Mol. Pharmac. – 1979. – Vol. 12. – P. 68-580.
• Hajos F. An improved method for preparation of synaptosomal fractions in high purity / F. Hajos // Brain Res. – 1975. – Vol. 93. – P. 485-489.
• Hill A.V. The combinations of hemoglobin with oxygen and with carbon monoxide I / A.V. Hill // Biochem. J. – 1913. – Vol. 7, № 5. – P. 471-480.
• Kratenko R.I. Crown-ethers toxicity parameters and their cumulative properties in short-term experiments / R.I. Kratenko // Біологія та валеологія. – 2016. – № 18. – Р. 38-43.
• Kratenko R.I. Crown-ethers – xenobiotics which possess membranotropic activity / R.I. Kratenko // Біологія та валеологія. – 2014. – № 16. – Р. 21-28.
• Lowry O.H. Protein measurement with the Folin phenol reagent / O.H. Lowry, N.J. Rosenbrough, A.L. Farr, R.J. Randall // J. Biol. Chem. – 1951. – Vol. 193. – P. 265-275.
• Peroutka S.J. Multiple serotonin receptors: differential binding of 3H-5-hydroxytryptamine, 3H-5-lysergic acid diethylamide and 3H-spiroperidol / S.J. Peroutka, S.H. Snyder // Mol. Pharmacol. – 1979. – Vol. 16. – P. 687-699.
• Rosenthal H.E. A graphic method for the determination and presentation of binding parameters in a complex system / H.E. Rosenthal // Analytical Biochem. – 1967. – Vol. 20. – P. 525-532.
• Scatchard G. The attractions of proteins for small molecules and ions / G. Scatchard // Ann. N.Y. Acad. Sci. – 1949. – V. 51. – P. 660-672.
• U’Prichard D. Binding characteristics of a radiolabeled agonist and antagonist of central nervous system alpha-noradrenergic receptors / D. U’Prichard, D. Greenberg, S.H. Snyder // Molec. Pharmac. – 1977. – Vol. 13. – P. 454-473.
• Vashovsky V.E. UPTL of phospholipids micstures containing phosphotidyl glycerol / V.E. Vashovsky, T.A. Terekkive // J. High Res. Chromatogr. – 1979. – № 11. – P. 671-672.

«Bulletin of problems biology and medicine» Issue 4 Part 3 (141), 2017 year, 79-83 pages, index UDK 613-615.092.044:616.099.036

10.29254/2077–4214–2017–4–3–141–79-83