EFFECT OF PLACENTA FREEZING ON ANTIOXIDANT ACTIVITY OF ITS EXTRACTS AGAINST ERYTHROCYTES IN OXIDATIVE STRESS

Narozhnyi S. V., Nardid O. A., Rozanova Ye. D., Pokryshko S. V.

CLINICAL AND EXPERIMENTAL MEDICINE

Scentific article

Antioxidant effect of extracts obtained from fresh and frozen-thawed human placenta against erythrocytes in oxidative stress caused by hydrogen peroxide was investigated in the study. It was determined, that extracts obtained from fresh placenta had antioxidant properties preserved after freezing. Objects of study. Human placenta obtained from healthy parturients with their informed consent, stored for no more than 24 hours at 4°C, was used in research. Water-salt extracts of human placenta were obtained by tissue homogenization and their further exposing to phosphate-buffered saline (pH 7.4). Gel chromatography was used for fraction obtaining. The erythrocyte mass obtained from the blood of male donors (A (II) Rh +) was used for oxidative stress modelling. Methods of research. Evaluation of the antioxidant activity of human placenta extracts and fractions was performed applying spectrophotometric method with discoloration of ABTS + cation radical. Protein concentration was recorded spectrophotometrically, by absorption at wavelength of 260 nm and 280 nm. The antioxidant effect of extracts and their fractions was investigated on the model of erythrocytes oxidative stress caused by hydrogen peroxide. Osmotic stability was evaluated by modified method of Gorshkov et al. Results and discussion. It has been determined, that preincubation of cells with extracts and some of their fractions increased the osmotic resistance of erythrocytes and reduced the level of hemolysis caused by the action of hydrogen peroxide on their membrane. Moreover, extracts obtained from placenta frozen to -196˚С with high speed, as well as extracts obtained from placenta, frozen to -20˚С with slow cooling rate retain their antioxidant effect. Most probably, the antioxidant effect of extracts is cased by the interaction of proteins and peptides with erythrocyte membrane. The research data regarding kinetics of ABTS + radical restoration by extracts and their individual fractions suggest, that their composition contains a significant amount of SH-groups, which prevent lipid oxidation in erythrocyte membrane.

human placental extracts, oxidative stress, hydrogen peroxide, antioxidants

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«Bulletin of problems biology and medicine» Issue 4 Part 3 (141), 2017 year, 168-172 pages, index UDK 618.46:57.086.13:577.121.7

10.29254/2077–4214–2017–4–3–141–168-172