GENE LSP1 POLYMORPHISM IN WOMEN WITH BREAST CANCER IN UKRAINE

Polinyk S. I., Rybchenko L. A., Zakhartseva L. M., Klymenko S. V.

CLINICAL AND EXPERIMENTAL MEDICINE

Scentific article

Breast cancer is caused by the interaction of various genetic factors and the environment. Genetic polymorphisms (SNPs) in some genes may alter the expression of mRNA and protein, and therefore, affect the susceptibility to cancer. The aim of the work was to investigate the frequency of rs3817198 polymorphism of the LSP1 gene in women of all ages from Ukraine with breast cancer patients. The main group of patients was formed from 158 women aged 35-60 years old with a diagnosis of breast cancer, which was confirmed histologically. The search for mutations was done using the PCR method and the analysis of the temperature melting point Tm of the amplicons obtained. The PCR reaction was performed using TIB MOLBIOL (Germany) reagents. The displacement of the melting point indicated changes in the nucleic acid sequence of the amplicon. The Tm analysis was performed using Light Cycler Software (build 4.1.1.21.) (Roche, Switzerland). Statistical calculations were performed using the StatPlus Pro program package. The TT genotype in the group of women with breast cancer with a radiation history and radiation history was more frequent (68.2 and 58.8%) than in the control group (48.0%), although the experimental groups showed less heterosisit after the genotype of ST (18.2 and 29.4% at 40.0% in the control group). A higher allele T frequency was found in the group of women with breast cancer with a radiation history (77.3%) and a lower frequency in the group of women with breast cancer without a radiation history (73.5%) compared with the control group (68.0%). The established distribution of genotypes and alleles for the polymorphic version of rs3817198 of the LSP1 gene in the first and second groups was not significantly different from the theoretically anticipated on the Hardy- Weinberg equilibrium. The statistical analysis of the odds ratio (OR) revealed an increased risk of developing breast cancer in the presence of the genotype of the polymorphic rs3817198 locus of the LSP1 gene in women aged ≤ 40-50 with a history of radiation (OR = 1.33), confidence intervals (IC 95%: 0.50-3.52). The results obtained by us confirm the association of genetic polymorphism of rs3817198 of the LSP1 gene in women ≤ 40-50 in patients with breast cancer with a radiation anamnesis. According to the results of the molecular genetic analysis of the genetic polymorphism rs3817198 of the LSP1 gene, there are no statistically significant differences in the frequencies of genotypes and alleles in the group of women with breast cancer patients with a radiation history, nor in women with breast cancer without a radiation history in the age of 51-60 years.

LSP1 gene, ionizing radiation, breast cancer

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«Bulletin of problems biology and medicine» Issue 4 Part 3 (141), 2017 year, 203-208 pages, index UDK 618.19-006:616-001. 28:575 (083.13)

10.29254/2077–4214–2017–4–3–141–203-208