BIOLOGICAL PROPERTIES OF STRAINS OF OPPORTUNISTIC MICROORGANISMS, ISOLATED DURING DYSBIOSIS OF GASTROINTESTINAL TRACT

Kazmirchuk A. A., Holodok L. P., Voronkova O. S.

MICROBIOLOGY

Scentific article

The study of the role of intestinal microflora in recent years has convincingly shown that it is the most important component of the protective intestinal barrier, which controls the interaction of the host organism and the environment. The gastrointestinal tract (GIT) represents one of the most complex ecosystems in the body of human, which has a very delicate balance between the cells of the digestive system and the microbial flora. The normal intestinal microflora is important for maintenance at the optimal level of metabolic processes occurring in the macroorganism, as well as for the creation of a high colonization resistance of the host organism in relation to pathogenic microbes. Causes that lead to a violation of the composition of the normal intestinal microflora are quite numerous and varied. The development of microecological disorders contribute to allergies, diseases of the digestive system, endocrine, immune disorders, stress, irrational nutrition and therapy, etc. The use of antibiotics significantly changes the “microbial landscape” of the gastrointestinal tract, often causing clinically significant consequences (pain syndrome, diarrhea). Under the term “intestinal dysbiosis” understand the qualitative and quantitative changes characteristic of the human microflora, which entails a pronounced clinical response of the macroorganism or is a consequence of any pathological processes in the body. The aim of the research was to investigate the human intestinal microflora during dysbiotic disorders and to identify the ability of isolated microorganisms to biofilm formation. The object of the study was the violation of human intestinal microbiocenosis and the ability of isolated strains to biofilm formation. The research was done on the basis of the Laboratory of Microbiology and Immunology of the State Research Institute of Gastroenterology of the National Academy of Medical Sciences of Ukraine in the city of Dnipro. Investigated strains were isolated from the material (feces) from person with suspected dysbiosis. A total of 44 samples of material were investigated. There were isolated 28 (64%) isolates of Staphylococcus aureus; 7 (16%) isolates of Esherichia coli; 5 (11%) isolates of Klebsiella spp.; 4 (9%) isolates of Proteus spp. The largest number of strains is grafted to S. aureus, therefore, for further investigation, 28 clinical isolates were used. The ability of strains of S. aureus to biofilm formation was investigated. The 86% of all isolates of staphylococci were film-forming, 14% were planktonic forms. At the next stage, a study was carried out to determine the sensitivity of isolated S. aureus isolates to antibiotic drugs by the disc-diffusion method. According to the results of the study, the sensitivity of isolated S. aureus strains they were susceptibile to vancomycin – 92.85%, cefuroxime – 78.57%, rifampicin – 71.4%, azithromycin – 28.57% and tetracycline – 0%. Given the belief that only bacteriological studies with suspicion of dysbiosis may not be sufficient, additional markers should be sought, including among the biological properties of microorganisms that may be associated with an increased risk of dysbiosis. First of all, it is a question of studying the adhesive properties and the ability of microorganisms to biofilm formation, which may be associated with an increase in their pathogenetic properties

dysbiosis, conditionally pathogenic microorganisms, biofilm, sensitivity to antibiotics

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«Bulletin of problems biology and medicine» Issue 4 Part 3 (141), 2017 year, 269-272 pages, index UDK 579.61

10.29254/2077–4214–2017–4–3–141–269-272