SPECTRUM OF PATHOGENS OF THE DISORDERS OF THE WOUND SURFACES AND THEIR SENSITIVITY TO ANTIBIOTICS

Shelest Yu. V., Sklyar Т. V.

MICROBIOLOGY

Scentific article

A significant number of surgical diseases and postoperative complications in children caused by purulent bacterial flora, is difficult and with a constant threat of sepsis, with high mortality and frequency of invalidating a sick child. The increased level of resistance of pathogens of wound infections to antimicrobial drugs should be taken into account when planning schemes for the treatment by antimicrobial drugs. In general, the improvement of the methods of prevention, early diagnosis and prediction of the current surgical bacterial infection in children, increasing the effectiveness of treatment of patients with purulent-septic pathology in our time is one of the most urgent problems of pediatric surgery in pediatrics. The aim of the research was to investigate the range of pathogens of lesions of wound surfaces in children and to determine their sensitivity to antibiotics. The object of the study was a material taken from wound surfaces of children (n = 45). Identification of microorganisms was carried out by generally accepted methods. In 35 out of 45 examined children, microorganisms were identified as: S. aureus – 7 (20.0%) strains, S. epidermidis – 10 (28.6%) strains, E. coli – 7 (20.0%) strains, Klebsiella spp. – 4 (11.4%) strains, Proteus spp. – 5 (14.3%) strains, P. aeruginosa – 2 (5.7%) strains, the presence of which was confirmed by bacteriological method. Sensitivity to antibiotics of isolated strains of bacteria was determined using a disk-diffusion method with standard disks of industrial production. All isolated staphylococci strains were sensitive to cephalosporins and fluoroquinolones, to which there were no stable isolates at all. The most effective against all isolated strains was ceftriaxone, to which more than 85% of the strains had complete sensitivity, and lincomycin and ampicillin were the least effective ones, to which no susceptible strains were detected at all. From the presented data, it can also be noted that strains of S. aureus differed slightly higher than the S. epidermidis strains. Representatives of the Enterobacteriaceae family, as well as staphylococci, are characterized by a high level of resistance to antibiotics, which requires constant monitoring of the proliferation of resistant strains and indicates the need to study the spectrum of sensitivity to antibiotics of clinical isolates for the purpose of the optimal therapeutic scheme. It was found that the least effective against E. coli was gentamicin, for Klebsiela spp. and Proteus spp. – tetracycline, to which the bacteria did not show sensitivity. Also tetracycline and furazolidone were also ineffective. High activity of E. coli was done with meropenem (85.7%), ceftriaxone (71.4%) and ciprofloxacin (42.8%). For isolates of Klebsiela spp. the drugs of choice are ciprofloxacin and meropenem, to which 75% of strains were susceptible and 50% sensitive to ceftriaxone. For Proteus spp. when determining antibiotic susceptibility, the following results were obtained: 80% sensitized to ceftriaxone, 60% to ciprofloxacin and meropenem; 100% of strains are tetracycline resistant. 100% of the pseudomonadal strains had sensitivity to meropenem and ciprofloxacin, and 50% to gentamicin. At the same time, 100% of strains were resistant to ceftriaxone, ampicillin and tetracycline, which makes it impossible for the latter to treat lesions of wound surfaces. That’s why, it is important to investigate the spectrum of sensitivity of each specific isolate to antibiotics, which will allow the development of a scheme of rational antibiotic therapy, and may also be the basis for the development of prevention measures for the spread of resistant strains.

wound infection, staphylococci, enterobacteria, pseudomonas, resistance to antibiotics

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«Bulletin of problems biology and medicine» Issue 4 Part 3 (141), 2017 year, 281-284 pages, index UDK 579.61

10.29254/2077–4214–2017–4–3–141–281-284