FEATURES OF THE EARLY STAGE OF MORPHOGENESIS OF WOUND HEALING IN CASE OF DIABETES MELLITUS DEPENDING ON METHOD OF WOUND CLOSURE

Loza E., Marushchak M.

MORPHOLOGY

Scentific article

Diabetes mellitus has been and remains a major problem for now. Any injury or surgical intervention is accompanied by the development of scar tissue. It is proved that the nature and type of scar depends on the processes occurring in the wound in the early postoperative period. The type of the used suture material has a huge influence on such processes. Purpose: to study the morphological features of the early period of skin wound healing in conditions of diabetes, depending on the used suture material. Object and methods. The study was conducted on 20 adult male rats, weighing 240-280 g, with diabetes (injections of streptozotocin (Sigma, USA) (intraperitoneally – 65 mg/kg) with a previous (15 min) injections of nicotinamide (intrathecal 230 mg/kg), 4-week maintenance of animals on a high-calorie diet). For all animals, under anesthesia, full-length rectilinear incisions of 2 cm in the anterior-abdomen were performed. For the closureof the postoperative wound, to the animals of the 1st experimental group (10 rats) used “Vikryl 5/0”, for the 2nd experimental group (10 rats) – skin glue Dermabond. Results. In the course of morphometric studies, cell density (in the 1st group) was determined to be 49.4 ± 0.43 at 10,000 microns, the greater the number of cells of the macrophage monocyte row, lymphocytes, plasma cells, which made up (76.5 ± 0.35)% of the total cells. Elements of the fibroblastic raw (23.5 ± 0.63)% and were represented by small specialized fibroblasts, with elongated form, basophilic cytoplasm and oval or round nucleus. The cellular cell density (in the 2nd group) was – 38.1 ± 0.27 in 10,000 μm2, which is significantly lower than in the previous group. The percentage of cells in the macrophage-monocyte row was (63.1 ± 0.33)% among all cells of granulation tissue, fibroblast number – (36.9 ± 0.43)%. Conclusion. According to experimental, histological and morphometric studies, it has been proved that the use of skin glue has been shown to accelerate the cleansing of the wound from tissue detritus, the epithelization and maturation of the granulation tissue is faster compared with the use of nodal sutures. In this case, the condition of the microvascular channel is more favorable for healing of the wound.

wound healing, wound morphogenesis, scar formation, diabetes mellitus, skin glue, histological examination, microscopic examination

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«Bulletin of problems biology and medicine» Issue 4 Part 3 (141), 2017 year, 311-314 pages, index UDK 616.379-008.64-003.93-084

10.29254/2077–4214–2017–4–3–141–311-314