ALIMENTARY VARIANTS OF THE MODEL OF CHRONIC PERIODONTITIS IN FEMALE RATS

Pogrebnyak G. V.

DENTISTRY

Scentific article

The work is devoted to the substantiation of the possibility of creating alternative variants of the model of chronic periodontitis in female rats. Purpose. Study of the possibilities of modeling chronic periodontitis in male rats alimentary way with different variants of combination of fats and carbohydrates. Object and methods. The studies were carried out during 7 months in 40 female rats of the Vistar line at the age of 4 months, weighing 159.95 ± 0.24 g. Animals were divided into 4 groups of 10 animals in each: Group 1 – Animal holding in full feed; Group 2 – additional administration of excessive amounts (up to 25%) of easily digestible carbohydrates with a total content of more than 40%. 3-rd and 4-th groups – additional administration of up to 35% of fat of vegetable and animal origin, respectively. The total content of crude fat in designated rations did not exceed 40% and was assessed as high. The withdrawal of animals from the experiment upon its completion was carried out in compliance with the conditions of eftanasias. In periodontal tissues, the content of triglycerides (TG), resorption of alveolar bone was determined. The results obtained were statistically processed using Student’s t criterion. Research results and their discussion. The level of TG in the alveolar bone, gingival tissue in animals of the 1-st group was considered as a conditional physiological norm (2.93 ± 0.81 mmol/g, 4.43 ± 0.96 mmol/g). Resorption was low intensity – 1 point. The level of TG in animals in the 2-nd group was 5.71 ± 0.12 mmol/g in the alveolar bone, 10.30 ± 0.94 mmol/g in the gingival tissue (P2.1≤0.01-0.05) Alveolar bone resorption is 2 to 3 points. The highest TG in the alveolar bone, compared to the control value, was observed in animals of the 3-rd group (8.79 ± 0.16 mmol/g, P3.1≤0.01-0.05). A similar tendency was observed in them and in the gums tissue (13.41 ± 3.23, P3.1≤0.05). Alveolar bone resorption is 3 to 4 points. It was established that the tendency of TG increase in periodontal tissues in animals of the 3-rd group was more pronounced than in the 2-nd group. Here in the alveolar bone increased the level of TG content was 1.5 times, and in the gums tissue – 1.3 times (P3,2≤0,01-0,05). In animals of the 4-th group, no significant difference was found between the levels of TG in the bone tissue of the alveolar appendix of the mandible and gingival tissue (P4.1≥0.05). The resorption of the bone tissue of the alveolar appendix of the mandible remained at the control level. Conclusions. Simulation of chronic periodontitis in female rats is possible under the conditions of an alimentary overload in a balanced diet, separately, easily digestible carbohydrates and vegetable fat. According to objective data, the introduction of a complete diet of females of rats with excessive amounts of easily digestible carbohydrates – up to 25% with a final content of more than 40% – is characterized by the presence of signs of insulin resistance in the investigated periodontal tissues and is accompanied by an intensive resorption process. Adding to a complete diet of female rats an excess amount of 35% of vegetable fat with a total fat content of up to 40% indicates a higher activity of experimental signs of chronic periodontitis (P3,2≤0,01-0,05). Excessive amount of animal fat (up to 35%) in ration of feeding female rats as an alternative version of the periodontitis model (P4,1≥0,05) proved to be insolvent.

models of chronic parodontitis, females of rats, carbohydrates, fats, alveolar appendix, gingival tissue, triglycerides, resorption

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«Bulletin of problems biology and medicine» Issue 4 Part 3 (141), 2017 year, 367-370 pages, index UDK 616.314.17-008.1-002.2:599.23.001.57.

10.29254/2077–4214–2017–4–3–141–367-370