SEXUAL BEHAVIOR OF MALE RATS WITH GONADOPATHY AFTER CORRECTION BY VITAMIN D 3 AND TRIBESTANE

SmolienkoN.P., Korenyeva Ye. M., MarakhovskiyI.O., ChystyakovaE.Ye., VelychkoN.F., BelkinaI.O., BondarenkoV.О.

CLINICAL AND EXPERIMENTAL MEDICINE

Scentific article

The solution to the current problem of sexual dysfunction in men is the study and implementation of new drug compositions The aim of this study was to determine the effectiveness of vitamin D3 co-administration with Tribestan in correcting sexual behavior disorders in male rats caused by experimental gonadopathy. The study was performed on sexually active seven-month-old males of the Wistar population weighing 250–300 g. Simulations of rat gonadopathy were performed by injection of Serotonin hydrochloride once daily subcutaneously for 14 days at a dose of 3.3 mg / kg. Tribestan was administered per os at a dose of 68 mg/kg body weight three days before serotonin hydrochloride administration, serotonin administration (14 days), and three days after the last serotonin injection. Vitamin D3 was given in a volume of 0.5 ml at a dose of 4000 IU (per os). Sexual behavior was studied at the end of serotonin injections when mating with an ovariectomized receptive female for 15 minutes at dusk in quantitative and temporal indices of intercourse, intromia, and ejaculation. It has been shown that in male rats experimental serotonin damage to the testes resulted in suppression of sexual behavior with the development of erectile dysfunction. Correction of sexual behavior of males with serotonin testicular lesions with vitamin D3 or Tribestan affected the peripheral mechanism of regulation, which was reflected in an increase in the proportion of rats capable of mating and an increase in the number of intrusions during the test. Correction of simulated serotonin gonadopathy in males by combined use of vitamin D 3 with Tribestan leads to restoration of mating ability, improvement of mating initiation and ejaculatory threshold. Influence is reflected in the elements of sexual behavior that are regulated at both the central and peripheral levels.

vitamin D3 , gonadopathy, sexual health, sexual behavior, Tribestan.

1. Skliarov PM, Fedorenko SY, Naumenko SV, Onischenko OV, Holda KО. Retinol deficiency in animals: etiopathogenesis and consequences. Regulatory Mechanisms in Biosystems. 2020;11(2):162–169. DOI: 10.15421/022024.
2. Enis RC, Burak O. Premature Ejaculation and Endocrine Disorders: A Literature Review. World J Mens Health. 2022;40(1):38–51. DOI: 10.5534/wjmh.200184.
3. Patrick RP, Ames BN. Vitamin D hormone regulates serotonin synthesis. Part 1: relevance for autism. FASEB J. 2014;28:2398–2413. DOI: 10.1096/fj.13–246546.
4. Brechka NM. Androhennyy status samtsiv shchuriv pislya zastosuvannya preparativ pryrodnoho pokhodzhennya v umovakh kriotravmy peredmikhurovoyi zalozy. Ukrayinsʹkyy zhurnal medytsyny, biolohiyi ta sportu. 2020;4(4):237–243. DOI: 10.26693/ jmbs04.04.237. [in Ukrainian].
5. Santos Jr CA, Reis LO, Destro-Saade R, Luiza-Reis A, Fregonesi A. Tribulus terrestris versus placebo in the treatment of erectile dysfunction: A prospective, randomized, double blind study Author links open overlay panel. Actas Urológicas Españolas. 2014;38(4):244–248. DOI: 10.1016/j.acuro.2013.09.014.
6. Mukhram MA, Rafiq M, Kumar N, Jadhav AN, Anturlikar SD, Sundaram R, et al. Sexual Behaviour in Male Healthy Rats: A Comparison Between Indian Tribulus terrestris Linn. Extracts with Protodioscin Standardized Bulgarian Extract. The Natural Products Journal. 2019;9(1):32–38. DOI: 10.2174/2210315508666180810144454.
7. Smolienko NP, Chystyakova EYe, Korenyeva YeM, Belkina IO, Velichko NF, Karpenko NO, et al. Stan kopulyatyvnoyi funktsiyi u shchuriv z khronichnym prostatytom pry korektsiyi kombinatsiyeyu nanochastynok ridkozemelʹnykh elementiv zyi sriblom. Liky – lyudyni. 2020;1:468–473. [in Ukrainian].
8. Tricklebank MD, Daly E, editors. The serotonin system: History, neuropharmacology, and pathology. Elsevier Academic Press; 2019. Chapter 7, Serotonin and sexual behavior; р. 117–132. DOI: 10.1016/B 978–0–12–813323–1.00007–4.
9. Pfaff D, Saad F. Sexual motivation: problem solved and new problems introduced. Hormone molecular biology and clinical investigation. 2020;41(2):20190055. DOI: 10.1515/hmbci‑2019–0055.
10. Horpynchenko II, Sytenko AM. Erektylʹna dysfunktsiya: etiolohiya, patohenez, diahnostyka ta metody likuvannya. Zdorovʹe muzhchyny. 2016;2(57):1–8. [in Ukrainian].
11. Northrop LE, Polston EK, Erskine MS. Noradrenergic nuclei that receive sensory input during mating and project to the ventromedial hypothalamus play a role in mating-induced pseudopregnancy in the female rat. J Neuroendocrinol. 2010;22(10):1061–1071. DOI: 10.1111/j.1365–2826.2010. 02049.x.
12. Abdel-Hamid IA, Ali OI. Delayed Ejaculation: Pathophysiology, Diagnosis, and Treatment. World J Mens Health. 2018;36(1):22–40. DOI: 10.5534/ wjmh.17051.
13. Di Sante S, Mollaioli D, Gravina GL, Ciocca G, Limoncin E, Carosa E, et al. Epidemiology of delayed ejaculation. Transl Androl Urol. 2016;5:541–548. DOI: 10.21037/tau.2016.05.10.

«Bulletin of problems biology and medicine» Issue 2 Part 1 (164), 2022 year, 240-248 pages, index UDK 612.616+591.463.12: 612.613+591.512.14

10.29254/2077-4214-2022-2-1-164-240-248