ANTIBACTERIAL ACTIVITY OF EXTRACTS OF VACCINIUM CORYMBOSUM L. SHOOTS

Yavorska H. V., Vorobets N. M., Yavorska N. Y., Fafula R. V.

BIOLOGY

Scentific article

Shoots of Vaccinium corymbosum contain rich bioactive phytochemicals, which makes them particularly valuable for pharmaceutical and medical applications. Since the content of bioactive compounds depends on the variety and the phase of development at which plant raw materials are selected, the Bluejay variety of highbush blueberry with early fruit ripening was selected for research, and the raw materials (shoots) were choosed at different stages of vegetation. This new approach was developed to determine the antibacterial activity of V. corymbosum shoot extracts. Shoots of V. corymbosum cv. Blujay, collected in the phases of flowering (I), fruiting (II), after fruiting (III) and the stage of winter dormancy (IV), dried, ground to a powdery state, and aqueous and aqueous ethanol (AE) extracts of various concentrations were prepared from them by maceration method. This crude extract was subjected to antibacterial activity against museum strains of gram-positive and gram-negative bacteria. Ethanol of various concentrations was used as solvent control, as well as antiseptic drugs Decasan, Rotocan, Tinctura Eucalypti, and Chlorophyllipt, Cipronex (ciprofloxacin) were used as standard positive controls. The agar diffusion method as adapted earlier using in modification by the wells and cylinders was used. The different antibacterial activity of the extracts has been proved: Extracts of shoots collected at the I stage of vegetation had low antibacterial activity against gram-positive and gram-negative bacteria, and lower compared to commercial preparations. The aqueous extract of shoots collected at the II stage of vegetation showed the highest antibacterial activity against: B. subtilis, M. luteus, P. fluorescens, P. vulgaris and S. albus, but not for E. coli. P. vulgaris and B. subtilis were highly sensitive to extracts of shoots with 70% and 80% AE (III stage); the extract with 50% AE had high antibacterial activity against P. vulgaris and M. luteus; antibacterial activity of extracts with 50-, 70-, 80% AE against all tested bacterial strains was higher than commercial drugs (except Cipronex). Shoot extract with 80% AE (IV stage of vegetation) showed high antibacterial activity against P. fluorescens and P. vulgaris, and strains of gram-positive bacteria showed moderate sensitivity to it. Extracts of high-growing blueberry shoots of the Bluejay variety, made with 60-80% aqueous ethanol, had high antibacterial activity against Escherichia coli, Bacillus subtilis, Staphylococcus albus, Pseudomonas fluorescens, Proteus vulgaris and Micrococcus luteus, so they can be considered promising for further research in the manufacture of new drugs.

Vaccinium corymbosum variety Bluejay,antibacterial activity

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«Bulletin of problems biology and medicine» Issue 3 (166), 2022 year, 120-128 pages, index UDK 582.688.31:615.32:615.281

10.29254/2077-4214-2022-3-166-120-128