Sensitivity to Antibiotics and Medicinal Products Bacteriophages the Filmforming and Non-Filmform- ing Strains of Staphylococcus Aureus

Lazarev E. V., Vorobey E. S., Voronkova O. S.

MICROBIOLOGY

Scentific article

As a result of the study of S. aureus 13 strains isolated from the reproductive tract of women, their ability to form biofilm, susceptibility to antibiotics and commercial preparations bacteriophages drugs were defined. Under the study of filmformation of experimental cultures for 8 strains (61.5 %) ability to form biofilm was showed. Under biofilms microscopy revealed distinct groups of staphylococcicells. Studied strains were most sensitive to cefazolin, imipenem, doxycycline, netylmicyn, clindamycin, ofloxacin and linezolid. Sensitivity to them varied from 84.6 % to 100.0 %. Low ranges of sensitivity were observed to benzylpeni­ cillin and erythromycin (23. 1 %). In our studies discovered that tooke place a differences in sensitivity to antibiotics between filmforming and non­filmforming strains. Sensitivity of eperimental strains to cefuroxime hadsignificantly difference. Among the filmforming strain sensitivity was less than 25 %, while among non­filmforming strains was higher up to 80 %. Sen­ sitivity to clindamycin had less difference: 75 % of the filmforming strains and 100 % non­filmforming strains were sensitive. In the case of linezolid opposite trend was shown. The sensitivity of filmforming strains was higher than non­filmforming strains (100 % and 80 % respectively). Study of the 13 strains of S. aureus sensitivity to piobacteriophage polyvalent, intesti­bacteriophages liquid and staphylococcal bacteriophage liquid showed that highest sensitivity was to staphylococcal bacteriophage liquid (46.2 %), the lowest – topiobacteriophage polyvalent (23.1 %). Sensitivity to bacteriophage drugs varied considerably in the filmforming and non­filmformingstrains. Sensitivity to staphylococcal bacteriophage of the filmforming strains was 37. 5 %, fornon­filmforming strains– 60 %. A similar pattern was observed for intesti­bacteriophage and piobacteriophage: sensitivity of non­filmforming strains was higher (60 % and 40 % respectively) comparatively to filmforming (25 % and 12.5 % respectively). Study of sensitivity to bacteriophages of S. aureus filmforming and non­filmforming strainsshowed that the abil­ ity to filmformation bind with less susceptibility to all studied phages drugs. Also fornon­filmforming strains ob­ served high sensitivity not only to staphylococcal bacteriophage liquid, but also to intesti – bacteriophage liquid. Our results indicate that the film­forming strains of bacteria more resistant to action of antibiotic and bacte­ riophages drugs make troublesin fighting with them. So promising is the continuation of more detailed studies of the impact on planctonic cultures and biofilms are formed by these strains of different substances, that will help to develop strategies to use them for treatment of the human diseases, caused by filmforming strains of bacteria.

staphylococci, filmformation, bacteriophages, antibiotics

• габриэлян н. и. исследование антибиотико­ и фагочувствительности нозокомиальных штаммов микробов, выделен­ных от пациентов трансплантологической клиники / н. и. габриэлян, е. М. горская, т. с. спирина [и др.] // Вестник трансплантологии и искусственных органов. – 2011. – т. ХІІІ, № 3. – с. 26­32.
• Мидленко В. и. чувствительность к препаратам бактериофагов возбудителей осложнений у больных после травм и оперативных вмешательств на опорно­двигательном аппарате / В. и. Мидленко, г. а. Шевалаев, и. М. ефремов // Фундаментальные исследования. – 2013. – № 9. – с. 871­874.
• немцева н. В. регуляция симбиотических взаимодействий в альго­бактериальных ассоциациях / н. В. немцева, е. а. селиванова, а. о. плотников [и др.] // Журнал микробиологии, эпидемиологии и иммунобиологии. – 2009. – № 4. – с. 62­66.
• определитель бактерий берджи в 2­х тт: пер. с англ. / под ред. дж. Хоулта, н. крига, п. снита, дж. стейли, с. уил­ льямса. – М.: Мир, 1997. – т. 2. – 368 с.
• практическое руководство по антиинфекционной химиотерапии / [под ред. л. с. страчунского, Ю. б. белоусова, с. н. козлова]. – смоленск : нииаХ сгМа, 2002. – 586 с.
• про затвердження методичних вказівок «Визначення чутливості мікроорганізмів до антибактеріальних препаратів»: наказ Міністерства охорони здоров’я україни № 167 від 05. 04. 2007 р. – к.: Моз україни, 2007. – 63 с.
• руководство по медицинской микробиологии. общая и санитарная микробиология. – кн. 1 / колл. авторов / под ред. а. с. лабинской, е. г. Волиной. – М.: биноМ, 2008. – 1080 с.
• чеботарь и. В. антибиотикорезистентность биопленочных бактерий / и. В. чеботарь, а. н. Маянский, е. д. кончакова [и др.] // клиническая микробиология и антимикробная химиотерапия. – 2012. – том 14, № 1. – с. 51­58.
• Costerton J. W. Bacterial biofilms: a common cause of persistent infections / J. W. Costerton, P. S. Stewart, E. P. Greenberg // Science. – 1999. – Vol. 284, № 5418. – P. 1318­1322.
• Costerton J. W. Microbial biofilms / J. W. Costerton, Z. Lewandowski, D. E. Caldwell [et al.] // Annual Review of Microbiology. – 1995. – Vol. 49. – р. 711­745.
• Donlan R. M. Biofilms: survival mechanisms of clinically relevant microorganisms / R. M. Donlan, J. W. Costerton // Clinical Microbiology Reviews. – 2002. – Vol. 15, № 2. – P. 167­193.
• Fedtke I. Bacterial evasion of innate host defenses – the Staphylococcus aureus lesson / I. Fedtke, F. Gotz, A. Peschel // International Journal of Medical Microbiology. – 2004. – Vol. 294, № 1­2. – P. 189­194.
• Kutateladze M. Bacteriophages as potential new therapeutics to replace or antibiotics / M. Kutateladze, R. Adamia // Trends in Biotechnology. – 2010. – Vol. 28, № 12. – P. 591­595.
• Mah T. F. Mechanisms of biofilm resistance to antimicrobial agents / T. F. Mah, G. A. O’Toole // Trends in Microbiology. – 2001. – Vol. 9. – P. 34­39.
• Monk A. B. Bacteriophage applications: where are we now? / A. B. Monk, C. D. Rees, P. Barrov [et al.] // Letters in Applied Microbiology. – 2010. – Vol. 51. – P. 363­369.
• Watnick P. Biofilm, city of microbes / P. Watnick, R. Kolter // Journal of Bacteriology. – 2000. – Vol. 182, № 10. – P. 2675­2679.

«Bulletin of problems biology and medicine» Issue 3 part 2 (111), 2014 year, 275-278 pages, index UDK 579. 61:616­078