Tkachenko A. S.

The Functional State of the Vascular Endothelium of the Small Intestine in Chronic Experimental Gastroenterocolitis

About the author:

Tkachenko A. S.



Type of article:

Scentific article


In recent years, there is a tendency to an increase of inflammatory bowel disease morbidity, including Crohn’s disease and ulcerative colitis. It has been established that features of a modern diet might be considered as a possible source of the development of these diseases. In particular, the food additive carrageenan (E407), which is used in the food industry as a thickener and a gelling agent, may be a potential etiologic agent of inflammatory bowel disease. Despite the developed model of chronic moderate carrageenan-induced gastroenterocolitis without necrotizing process using low doses of carrageenan and investigation of some features of carrageenan-induced inflammation in the intestine, the mechanisms of this disease and especially of the small intestine damage remain unclear. In particular, the state of the vascular endothelium of the small intestine in carrageenan-induced intestinal inflammation is not studied. The aim of the investigation was to study the levels of endothelin-1 and S-nitrosothiols in the homogenate of the small intestine of rats with chronic carrageenan-induced gastroenterocolitis. The female Wistar rats were used for the experiment. Chronic carrageenan-induced gastroenterocolitis was reproduced by the free access of animals to 1 % solution of carrageenan in drinking water. Laboratory animals were divided into 3 groups. Group № 1 consisted of experimental animals, who consumed food additive carrageenan during 2 weeks. Group № 2 consisted of experimental animals, who consumed food additive carrageenan during one month and group № 3 consisted of intact animals. The development of gastroenterocolitius was proved morphologically and biochemically. Manipulations with animals were carried out in accordance with the provisions of the European Convention for the Protection of Vertebrate Animals used for Experimental and other Scientific Purposes (Strasbourg, 1986). The concentration of endothelin-1 in the intestinal homogenate was determined by ELISA using the reagent kit of the company Amersham (United Kingdom). The level of S-nitrosothiols in the small intestine homogenate was measured spectrofluorometrically. Results. Determination of the level of endothelin-1 in the small intestine homogenate of experimental animals from the first group showed a significant 1.8 times increase of its level, and 2.9 times increase in animals of the second group compared to control animals. The statistically significant slight elevation of S-nitrosothiols (19 %) in animals of the first group was found compared to rats of the control group. The progression of the disease led to decrease of S-nitrosothiols level – rats of the second group had a 42 % decrease of S-nitrosothiols level compared to the control group. Conclusions. Obtained data suggest the presence of endothelial dysfunction in carrageenan-induced inflammation of the intestine and it plays an important role in the pathogenesis of the disease.


gastroenterocolitis, carrageenan, rats, endothelin-1, S-nitrosothiols


  • Ватутин Н. Т. Эндотелины и сердечно-сосудистая патология / Н. Т. Ватутин, Н. В. Калинкина, А. Л. Демидова // Укр. кардіол. журн. – 2006. – № 1. – С. 101-106.
  • Жуков В. И. Система перекисного окисления липидов и активность апоптоза при экспериментальном хрониче- ском гастроэнтероколите / В. И. Жуков, А. С. Ткаченко // Науч. Вед. Бел. ун. Медицина. Фармация. – № 18 (161), Вып. 23. – С. 138-141.
  • Загорский С. Э. Хронические воспалительные заболевания у детей и подростков (современный подход) / С. Э. За- горский, Л. М. Беляева // Минск : БелМАПО. – 2007. – С. 4-28.
  • Патент на винахід № 97322 от 25.01.12 «Спосіб моделювання хронічного гастроентероколіту».
  • Ткаченко А. С. Уровень VEGF и ФНО-α при хроническом каррагенан-индуцированном гастроэнтероколите/ А. С. Тка- ченко // Вісн. пробл. біол. і мед. –2013. – Том 2 (103), Вип. 3. – С. 231-234.
  • Ekbom A. The epidemiology of IBD / A. Ekbom // Inflamm. Bowel. Dis. – 2004. – Vol. 10 (Suppl. 1). – P. 32-43.
  • Ferlay J. Cancer Incidence, Mortality and Prevalence Worldwide. / J. Ferlay, F. Bray, P. Pisani [et al.] // Globocan 2000, Version 1.0., IARC CancerBase No 5 Lyon, IARCPress – 2001.
  • Irvine E. J. A critical review of epidemiological studies in inflammatory bowel disease. / E. J. Irvine, F. Farrokhyar, E. T. Swarbrick // Scand. J. Gastroenterol. – 2001. – Vol. 36 (1). – P. 2-15.
  • Marzining M. Improved methods end products of nitric oxide in bioligical fluids: nitrites, nitrates ans S-nitrosothiols / M. Mar- zining, A. K. Nussler, J. Stadler // Nitric oxide. – 1997. – Vol. 1. – P. 177-189.
  • Ries L. A. G. SEER Cancer Statistics Review 1973–1996. Bethesda / L. A. G. Ries, C. L. Kosary, B. F. Hankey // National Cancer Institute. – 1999. – 60 p.
  • Ruetten H. Endothelin-1 stimulates the biosynthesis of tumour necrosis factor in macrophages: ET-receptors, signal trans- duction and inhibition by dexamethasone. / H. Ruetten, C. Thiemermann // J. Physiol. Pharmacol. – 1997. – №. 48. – P. 675- 688.

Publication of the article:

«Bulletin of problems biology and medicine» Issue 3 part 3 (112), 2014 year, 204-207 pages, index UDK 616. 33:616. 348]-002. -018. 74-092. 9