Influence of Immunomodulators and Antimicrobial Agents on Immune Cytokine Balance in Experimen- tal Purulent-Septic Process Caused by S. aureus

Mishina M. M.

MICROBIOLOGY

Scentific article

Introduction. Pyoinflammatory complications are still considered to be one of the most topical gen- eral clinical and surgical issues which is characterized by increase of patients in the number, high mortality rate and significant economic expenses. Sepsis constitutes currently a serious threat due to frequency as well as extremely severe course caused by pathogenicity factors of S. aureus. The goal of the research is determination of immune cytokine state in experimental purulent-septic process caused by S. aureus in case of use of Polyoxidonium, Roncoleukin and Gatifloxacin. The object and methods of the research. Experimental research which has been carried out on 30 mice of in- bred lines C57Bl/6JSto in accordance with the principles of European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes. The immune cells were differentiated by means of monoclonal antibodies to different types of the cells of Serotec Company. The results of the research and its consideration. In consequence of the research apparent deficit of absolute number of leukocytes, lymphocytes, cells with CD3+, CD4+ phenotypes and increase of lymphocytes with suppres- sive (CD8+), killer (CD16+) activity; as well as reduced indicator value of immunoregulation index with simultaneous increase of CD95+ cells indicating activation of programmed death of lymphocytes have been established. Sub- sequently, enhancement of apoptosis results in further deep changes and progression of T-lymphocutes deficit and imbalance of all parts of the immune system in the infected animals. At the same time decrease of expression of CD25+ marker on the surface of T-cells, which is α-chain of the receptor for ІL-2, was detected in the infected animals. Therefore processes of activation of apoptosis in apparent T-cell deficit is an important pathogenic as- pect in purulent-septic process caused by S. aureus. High activity of inflammatory response is proved by increase of production of ІL-1β and TNFα. Increase of IL-4 level in the blood serum, which is considered to be an indirect sign of active involvement of humoral immune response into pathogenesis of septic process caused by S. aureus, has been observed. Imbalance of cytokine status tends to maintain acute immune-dependent inflammation and concomitant disorders of processes of apoptosis and to lead to development of interleukin-dependent secondary immunodeficient state. Pathological states, which result from or are accompanied by secondary immune insufficiency, need adequate immunocorrection. In treatment of the infected animals by means of combination of medications the indices of cy- tokine status are normalized. It shows that the medications lead to evident therapeutic effect and completely restore immune processes in the body. As the key chain of immune defense is the phagocytic one, which is responsible for providing of reactions at early stages of purulent-septic processes, correction of this immunity chain is required. Through analysis of the indices of non-specific resistance, the tendency to increase of phagocytic, absorptive and migration function of neutrophils in the patients who undergo combined antimicrobial treatment by means of Poly- oxidonium, Roncoleukin and Gatifloxacin and vitamins B6 has been established. Consequently the results of the research show that this therapy provides positive effect for the immune system which is proved by decrease of IgМ and IgG concentration as well as CIC level, phagocytic activity and migration ability of neutrophils. Future research perspective in this direction is determination of metabolic state viz. antioxidant system and peroxidation of lipids in purulent-septic process caused by S. aureus with correction and elaboration of schemes of combination therapy.

purulent – septic process, S. aureus, immune cages

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«Bulletin of problems biology and medicine» Issue 3 part 3 (112), 2014 year, 241-244 pages, index UDK 579. 862. 1:615. 37:615. 33:612. 017:616-002. 3-092. 9