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    Bulletin of problems biology and medicine / Issue 4 part 1 (113), 2014 / Natural Origin Immunomodulators Influence on Protein Fractions, Cortisol and Glutathione Content in Broiler Chickens Blood under Stress

    Grabovskyi S. S. , Grabovska O. S.

    Natural Origin Immunomodulators Influence on Protein Fractions, Cortisol and Glutathione Content in Broiler Chickens Blood under Stress

    About the author:

    Grabovskyi S. S. , Grabovska O. S.

    Heading:

    BIOLOGY

    Type of article:

    Scentific article

    Annotation:

    The results of determination of protein fractions, cortisol and glutathione content in blood of broiler chickens, which further added to the feed of natural origin biologically active substances are presented in the article. As an antistressors and immunomodulators in pre-slaughter period are using of spleen extract biologically ac- tive substances were obtained with and without ultrasound application. The purpose of research – determination of changes of protein fractions, cortisol and glutathione content in broiler chickens blood before slaughter and their correction of natural origin biologically active substances (spleen extract). Object and research methods. The experiment was conducted on 15 broiler chickens with standard diet. Three groups of broiler chickens one month of age (5 chickens each) was formed for research. The spleen extract was obtained with (I research group) and without ultrasound application (II research group), were using as an biologically active substances to the feed broiler chickens in pre-slaughter period (five days before slaughter). The extracts were applied to feed by aerosol method (70 ° alcohol solution of spleen extract volume of 1.4 ml per chicken). The control group chickens (group III) received to the feed in the same way of 70 ° alcohol solution in the same volume. The feed eating by broiler chickens was exercised daily. The broiler chickens ate food completely. The broiler chickens slaughter was carried out in the morning. The blood plasma protein fractions separation was carried out by horizontal electrophoresis in polyacrylamide gel (PAAG). Cortisol level were determined in broiler chickens blood plasma. Glutathione concentration were determined in broiler chickens blood. Mathematical treatment of the research results worked statistically using the software package Statistica 6.0 and Microsoft Excel for Windows XP. Probability differences was assessed by Student t-test and results considered likely at P 0.05. Results and discussion. We measured the ratio of blood plasma protein fractions of broiler chickens, which in addition to the feed fed of natural origin biologically active substances. As a result of research was found that aerosol introduction of the spleen extract to the broiler chickens feed increases the γ-globulin concentration in broiler chickens blood plasma of the two experimental groups compared to the control. Cortisol level in broiler chickens (which further added to the feed of spleen extract obtained with ultrasound ap- plication (I research group) blood plasma was reliable lower (Р < 0. 05) compared to control, that may to indicate decreasing of stress before slaughter. The free cortisol level decreasing in broiler chickens blood plasma, perhaps, is associated with increasing of globulin fraction content. The glutathione level was found reliable higher (Р < 0. 05) in the broiler chickens blood of the control group. On the poultry feeding final stage is necessary to consider a pre-slaughter stress and to apply of natural origin biologically active substances. The results which obtained can to use in researches of cell immunity indices and stress hormones, such as cortisol, on farm animals for organism resistance increasing, correction and avoid their pre-slaughter stress and improve product quality.

    Tags:

    broiler chickens, pre-slaughter stress, spleen extract, ultrasound, protein fractions, cortisol, glutathione

    Bibliography:

    • Азарова Т. О. Динамика стресс-ассоциированных гормонов и показателей антиоксидантной защиты у молодняка кросса «Шейвер белый» / Т. О. Азарова, Л. Л. Осипова, С. Ю. Зайцев [и др.] // Птица и птицепродукты. – 2013. – № 1. – С. 25-29.
    • Байдевлятов А. Б. Методические рекомендации по профилактике стрессов в птицеводстве / А. Б. Байдевлятов, В. П. Николаенко, Л. А. Ольховик. – Харьков, 1979. – 13 с.
    • Влізло В. В. Лабораторні методи досліджень у біології, тваринництві та ветеринарній медицині : довідник / В. В. Влізло, Р. С. Федорук, I. Б. Ратич [та ін.]; За ред. В. В. Влізла. – Львів : Сполом, 2012. – 764 с.
    • Грабовський С. С. Вміст окремих класів ліпідів у крові курчат-бройлерів при передзабійному стресі / С. С. Грабовський // Біологія тварин. – 2013. – Т. 15. – № 4. – С. 24-31.
    • Заводник И. Б. Изменения во времени параметров респираторной активности и системы антиоксидантной защиты митохондрий печени крыс при острой интоксикации и диабете. Кислород и свободные радикалы : материалы Ре- спубликанской научно-практической конференции / И. Б. Заводник, Е. А. Лапшина, В. Т. Черевик [и др.] // Под ред. В. В. Зинчука. – Гродно : ГрГМУ, 2014. – 228 с.
    • Клімова О. Зміна співвідношення білкових фракцій сироватки крові у експериментальних тварин різного віку після імунізації цитотоксичною сироваткою хворих з аутоімунним захворюванням – міастенією / О. Клімова, О. Звягінцева, А. Малишев // Вісн. Львів. ун-ту. Сер. біол. – 2011. – Вип. 55. – С. 27-33.
    • Alberghina D. Changes in serum total proteins, protein fractions and albumin-globulin ratio during neonatal period in goat kids and their mothers after parturition / D. Alberghina, C. Giannetto, I. Vazzana [et al.] // Annals of Animal Science. – 2011. – Vol. 11(2). – P. 251-260.
    • Aoyama K. Regulation of neuronal glutathione synthesis / K. Aoyama, W. Masahiko, N. Toshio // Journal of pharmacological sciences. – 2008. – Vol. 108. 3. – P. 227-238.
    • Breuner C. W. Plasma binding globulins andacute stress response / C. W. Breuner, S. E. Lynn, G. E. Julian [et al.] // Hormones and Metabolic Research.– 2006. – Vol. 38. – P. 260-268.
    • Chowhan R. K. Polyamines in modulating protein aggregation / R. K. Chowhan, L. R. Singh // Journal of Proteins & Proteomics. – 2013. – Vol. 3(2). – P. 160-162.
    • Das S. K. Effects of long term ethanol consumption on cell death in liver / S. K. Das, S. Mukherjee, D. M. Vasudevan // Clin. Biochem. – 2011. – Vol. 26, № 13. – P. 84-87.
    • Dringen R., Hirrlinger J. Glutathione pathways in the brain / R. Dringen, J. Hirrlinger // Biol. Chem. – 2003. – Vol. 384. – P. 505-516.
    • Fokidis H. B. Corticosterone and corticosteroidbinding globulin in birds: relation to urbanization in a desert city / H. B. Fokidis, M. Orchinik, P. Deviche // General and Comparative Endocrinology. – 2009. – Vol. 160. – Р. 259-270.
    • Kaneko J. J. Serum proteins and the dysprotidemias. In : Clinical biochemistry of domestic animals. 5th Ed. / J. J. Kaneko. - London, UK : Academic Press, 1997. – Р. 117-138.
    • Keelan J. Quantitative imaging of glutathione in hippocampal neurons and glia in culture using monochlorobimane / J. Keelan, N. J. Allen, D. Antcliffe [et al.] // J. Neurosci. Res. – 2001. – Vol. 66(5). – P. 873-884.
    • Lynn S. E. The effects of short-term fastingon activity, corticosterone, and corticosterone binding globulin in amigratory song- bird, Gambel’s white-crowned sparrow (Zonotrichia leucophrys gambelii) / S. E. Lynn, C. W. Breuner, J. C. Wingfield [et al.] // Hormones and Behavior. – 2003. – Vol. 43. – Р. 150-157.
    • Maher P. The effects of stress and aging on glutathione metabolism / P. Maher // Ageing research reviews. – 2005. – Vol. 4.2. – Р. 288.
    • Malisch J. L. How acute is the acute stress response? Baseline corticosterone and corticosteroid-binding globulin levels change 24h after an acute stressor in Japanese quail / J. L. Malisch, D. G. Satterlee, J. F. Cockrem [et al.] // General and comparative endocrinology. – 2010. – Vol. 165(2). – Р. 345-350.
    • Marti O. Inhibition of corticosteroid-binding globulin caused by a severe stressor is apparently mediated by the adrenal but not by glucocorticoid receptors / O. Marti, M. Martin, A. Gavalda // Endocrine. – 1997. – Vol. 6. – Р. 159-164.
    • Matti V. Interaction of polyamines with proteins of human plasma: a preferential aggregation of fibrinogen and fibronectin (cold insoluble globulin) / V. Matti, S. Eija, R. Pekka // Biochimie. – 1980. – Vol. 62. – Р. 99-104.
    • Minni A. M. Critical role of plasma corticosteroid-binding-globulin during stress to promote glucocorticoid delivery to the brain: impact on memory retrieval / A. M. Minni, R. Dorey, C. Piйrard [et al.] // Endocrinology. – 2012. – Vol. 153(10). – Р. 4766-4774.
    • Navarro J. Blood glutathione as an index of radiation-induced oxidative stress in mice and humans / J. Navarro, E. Obrador, J. A. Pellicer [et al.] // Free Radical Biology and Medicine. – 1997. – Vol. 22(7). – Р. 1203-1209.
    • Official Journal of the European Union L276/33. DIRECTIVE 2010/63/EU OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 22 September 2010 on the protection of animals used for scientific purposes. 86/609/EC. 20. 10. 2010.
    • Poosuwan K. Long-term feeding effects of dietary protein levels on egg production, immunocompetence and plasma amino acids of laying hens in subtropical condition / K. Poosuwan, C. Bunchasak, C. Kaewtapee // Journal of animal physiology and animal nutrition. – 2010. – Vol. 94. 2. – Р. 186-195.
    • Qian X. A rapid release of corticosteroid-binding globulin from the liver restrains the glucocorticoid hormone response to acute stress / X. Qian, S. K. Droste, M. Gutiиrrez-Mecinas [et al.] // Endocrinology. – 2011. – Vol. 152(10). – Р. 3738-3748.
    • Reeder D. M. Stress in free-ranging mammals:integrating physiology, ecology, and natural history / D. M. Reeder, K. M. Kram- er // J. Mammal. – 2005. – Vol. 86. Р. 225-235.
    • Ritchie R. Wellness assessment: Targeted testing for specific problems which are not the measurement of health / R. Ritchie // Serum Proteins in Clinical Medicine. Clinical Section. – Foundation for Blood Research. Publishers: Scarborough. – 1999. - Vol. IІ. – P. 120. 00-1-120. 00-9.
    • Rosa A. R. Altered plasma glutathione levels in bipolar disorder indicates higher oxidative stress; a possible risk factor for illness onset despite normal brain-derived neurotrophic factor (BDNF) levels / A. R. Rosa, N. Singh, E. Whitaker [et al.] // Psychological medicine. – 2014. – Р. 1-10. – Код доступу http://www. psych. ox. ac. uk/publications/447561.
    • Rossi R. Oxidized forms of glutathione in peripheral blood as biomarkers of oxidative stress / R. Rossi, I. Dalle-Donne, A. Mil- zani, D. Giustarini // Clinical chemistry. – 2006. – Vol. 52(7). – Р. 1406-1414.
    • Sanandam M. R. Impact of endotoxin on physiological responses of rat exposed to textile environment / M. R. Sanandam // International Journal of Pharmaceutical Applications. – 2011. – Vol. 2. 3. – Р. 155-158.
    • Sapolsky R. M. How do glucocorticoids influence stress responses? integrating permissive, suppressive, stimulatory, and preparative actions / R. M. Sapolsky, L. M. Romero, A. U. Munck. – Endocr. Rev. – 2000. – Vol. 21. – Р. 55-89.
    • Thrall M. A. Laboratory evaluation of plasma and serum proteins. In : Veterinary hematology nd clinical chemistry. (Ed.) / M. A. Thrall. -  Philadelphia, USA : Lippincott Williams and Wilkins, 2004. – Р. 401-415.
    • Tinnikov A. A. Responses of serum corticosterone and corticosteroid-binding globulin to acute and prolonged stress in the rat / A. A. Tinnikov // Endocrine. – 1999. – Vol. 11. – Р. 145-150.

    Publication of the article:

    «Bulletin of problems biology and medicine» Issue 4 part 1 (113), 2014 year, 57-61 pages, index UDK 636. 52/. 58:519. 11:612. 176