Peculiarities of Immunomorphological State in Purulent-Septic Process Caused by S. aureus and under the Influence of Combination Therapy (experimental research)

Mishina M. M.

CLINICAL AND EXPERIMENTAL MEDICINE

Scentific article

Introduction. At the present day full attention is given to pathogenesis of disorders of immune re- sponse and its correction in pyoinflammatory processes caused by S. aureus. The secondary immune deficiency in this pathology is grounded on several mechanisms: death of population cells, imbalance of cell populations, func- tional cell blocking of the receptors due to binding of receptor apparatus by microbial toxins. The goal of this research is studying of the peculiarities of cell immunity in the tissues before and after combina- tion therapy which includes immunomodulators, cytokine correctors and antimicrobial agents. The object and methods of the research. Experimental research conducted on 80 mice of inbred lines C57Bl/6JSto. The experiments were carried out according to the national General Ethical Principles for Experi- ments on Animals (Ukraine, 2001) which are agreed upon by the principles of European Convention for the Protec- tion of Vertebrate Animals used for Experimental and Other Scientific Purposes (Strasbourg, 18. 03. 1986). The immunomorphological research was conducted on the paraffin sections with thickness of 5-6 μm by the indirect method of Kuns according to Brosman technology. Immune cells were differentiated by means of monoclonal an- tibodies to different types of cells of Serotec Company. The number of producer cells of cytokines was calculated within sight of ×400. The results of the research and its consideration. In immunomorphological research it has been established that the viscera of the infected laboratory animals is characterized by presence of producer cells of cytokines. Moreover its number is increased in comparison with the group of intact animals. Manifestation rate of macrophage reaction, reaction of plasmatization as well as content ratio of producer cells of cytokines is probably higher of those ones in intact animals. Intravascular fixation of neutrophilic granulocytes to the surface is observed. The number of plasmoblasts and producer cells of interleukins IL-1β, IL-6, TNF-α is increased. The walls of the vessels are with mediated fibrinoid necrosis; proliferation of the endothelium is apparent. The microscopic study of the tissues of internal organs has shown hyperemia of the blood capillaries and edema of weak fuchsinophil stroma. In treatment of the animals by antimicrobial agent Gatifloxacin and immunomodulator Polyoxidonium with cy- tokine corrector Roncoleukin, significant changes in the microscopic picture of the internal organs in comparison with the groups of animals, which underwent treatment only by antimicrobial agent with immunomodulator or an- timicrobial agent and cytokine corrector, are detected. Edema of moderately fuchsinophil stroma is reduced; its inflammatory infiltration is either absent, or is observed in the form of fine-focal perivascular lymphoid cellular infil- trates with low reactions to RNP and DNP in the cytoplasm and nucleus. Relevant volume of inflammatory infiltration is reliably decreased in comparison with the group of infected animals. In its composition all clones of the immune cells are found. Among the elements of inflammatory infiltration relevant volume of macrophages, plasmoblasts and producer cells of anti-inflammatory cytokines is reduced. The tissues of internal organs are not characterized by destructive changes and the dystrophic ones are not significant. The walls of vessels are fuchsinophil, without destructive proliferative changes; the basal endothelial membrane is moderately PAS-positive and has type IV col- lagen. Endotheliocytes show moderate luminous intensity. Conclusion. Consequently, due to use of combination therapy which includes antimicrobial agent, immuno- modulator and cytokine corrector, the organs of the immune system are characterized by recovery of structure and reduction of morphological manifestation of antigen exposure.

purulent-septic process, S. aureus, immunomorphological state

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