COMPARATIVE CHARACTERISTICS OF THE ANTIMICROBIAL ACTIVITY OF MATERIALS FOR TEMPORARY ROOT CANAL OBTURATION

Ryabokon E., Zhdanova N.

MICROBIOLOGY

Scentific article

The article presents a comparative description of the antibacterial activity of various pastes for temporary root canal obturation. One of the main goals of endodontic treatment is the complete removal of microorganisms from the root canal system. This is achieved by machining, the use of antibacterial irrigant, adequate filling of the free space and the possible use of antimicrobial dressings between visits. Numerous studies have shown that the application of a temporary obturation of root canals of the tooth to improve the efficiency of endodontic treatment. A wide range of products for the temporary sealing complicates the choice of optimal material for dentists. One of the important aspects of clinical efficacy materials is their antimicrobial activity. In this regard, a comparative characteristics of the antibacterial effect of different materials for temporary obturation of root canals of the tooth is an urgent task of modern restorative dentistry. The purpose of research — a comparative characterization of the antibacterial activity of drugs for temporary root canal obturation in vitro. To determine the antimicrobial activity we used medications for temporary root canal obturation with the mechanism of action is connected with the hydrolysis reaction and highly alkaline Ph. Reference strains of Candida albicans ATCC 885-653, Staphylococcus epidermidis ATCC 14990, Escherichia coli ATCC 25992, Pseudomonas aeruginosa ATCC 27853, Enterococcus faecalis ATCC 6783 were used as test cultures. The study was conducted using the method of "wells" (agar diffusion method). This method is based on the ability of the drug and its active ingredient to diffuse into the agar, at which seeding is performed the test culture study. Each material was assigned a serial number: 1 — «Metapex», 2 —«Calasept», 3 — «Apeksdent without iodoform», 4 — «Apeksdent with iodoform», 5 — «Yodotemp 25», 6 — «Endoyod». Crops incubated in an incubator at 37°C. The observations and the calculations performed for 3 days in the zones around the wells growth delay (in mm). If there is a delay zone 11 mm attributed to inactive drug categories, from 11 to 16 mm — moderately active and more than 16 mm — the active agent. Drugs of domestic production have strong antibacterial properties to Candida albicans ATCC 885-653, Staphylococcus epidermidis ATCC 14990, Escherichia coli ATCC 25992, Pseudomonas aeruginosa ATCC 27853, Enterococcus faecalis ATCC 6783. They can be recommended for the treatment of infected canals. From the group of materials based on calcium hydroxide antibacterial activity to Candida albicans ATCC 885-653, Escherichia coli ATCC 25992, and Enterococcus faecalis ATCC 6783 showed drug «Calasept». It was also found that calcium hydroxide based materials have high diffusion ability. Composite materials based on calcium hydroxide and iodoform pronounced antibacterial activity to reference strains studied showed no, except for steps 6783 Enterococcus faecalis ATCC. All drugs studied possess high antibacterial activity against Enterococcus faecalis museum strain ATCC 6783 as after incubation in thermostat remained in a petri dish only single colonies of microorganisms. From the group of materials based on calcium hydroxide antibacterial activity to Candida albicans ATCC 885-653, Escherichia coli ATCC 25992, and Enterococcus faecalis ATCC 6783 showed drug «Calasept». It was also found that calcium hydroxide based formulations have a high diffusion ability. It was found that the highest antibacterial activity possess drugs «Iodotemp 25» and «Endoyod», showing growth inhibition zones corresponding to the current active drug, all the studied strains. This allows us to recommend drugs for the treatment of severely infected canals. All of the studied drugs have a high antibacterial activity with respect to the museum strain Enterococcus faecalis ATCC 6783, leaving only a few colonies.

temporary obturation, antibacterial activity, root canals

• 1. Арутюнов С. Д. Подбор и применение новых антисептических препаратов для эндодонтического лечения хронического периодонтита / С. Д. Арутюнов, В. Н. Царев, А. С. Носик, Э. Г. Маргарян // Российский стоматологический журнал. — 2007. — № 3. — С. 4.
• 2. Беер Р. Иллюстрированный справочник по эндодонтологии / Р. Беер, М. А. Бауман, А. М. Киельбаса; пер. с нем.; под ред. Е. А. Волкова. — М.: Медпресс-информ, 2006. — 240 с.
• 3. Дмитриева Л. А. Новые тенденции в лечении верхушечного периодонтита / Л. А. Дмитриева, Т. В. Селезнев // Эндодонтия today. — 2004. — №1-2. — С. 30-31.
• 4. Иванченко О. Н. Клиническое исследование эффективности лечения хронического апикального периодонтита / О. Н. Иванченко, C. B. Зубов, Е. В. Иванова, В. И. Спицина // Российский стоматологический журнал. — 2008. — № 5. — C. 33-36.
• 5. Минцер О. П. Методы обработки медицинской информации / О. П. Минцер, Б. Н. Угаров, В. В. Власов. — К.: Вища школа, 1982. — 158 с.
• 6. Siqueira J. F. Jr. Bacterial reduction of infected root canals process of 2.5% NaOCl as irrigation and calcium hydroxide / camphor paramonochlorophenol paste, as inner-ear headband / J. F. Jr Siqueira, K. M. Magalhães, I. N. Rocas // J. Endod. — 2007. — № 33. — P. 667-672.

«Bulletin of problems biology and medicine» Issue 1 part 1 (126), 2016 year, 260-263 pages, index UDK 616.314.163-022.36-089.843-74:615.015.8