EXPERIMENTAL SUBSTANTIATION OF USE OF KETAMINE FOR PREVENTION HYPERALGESIA

Dmytriiev D. V.

MORPHOLOGY

Scentific article

The aim of the study was to morphologically justify the use of ketamine for the prevention of hyperalgesia. Methods. The study used 50 nonlinear rats weighing 200-250 grams in a model of hyperalgesia used karagenin edema (0.1 mL of 1% of the district). Karaheninovyy swelling reproduced sublantarnym introduction of 0.05 ml of 1% solution karagenin (Sigma, USA) in the right hind limb of the animal. Monitoring the development of prostaglandins edema in animals of both sexes were carried out at 3 o'clock (the height of) after flohohenu. The animals were divided into 4 groups. And — karagenin edema untreated (control) animals in group II/wat injected respectively fentanyl (5 CIM/kg), Group IV animals — ketamine (10-25 mg/kg). Removed material recorded 10% aqueous solution of neutral formalin for 48 hours, then it running water, subsequently conducted its dewatering system polyhydric alcohols and fill in paraffin by standard procedure. Prepared 7-8 microns thick sections stained with hematoxylin and eosin (basic method of staining of histological preparations) for the evaluation of pathological changes and reactions in the tissues of them aseptic inflammation disorders in response to the edema drug. Also histological preparations made from pieces of tissue, taken at 3-4 hour and 7-14 -day of the experiment than the traditional method of staining with hematoxylin and eosin, silver by Fouta method for determination of the tissues in the resolution of small nerve endings inflammation and pathological their changes. Microscopic histological preparations was performed using a light microscope OLIMPUS BX 41 using increments of 40, 100, 200 and 400 times. At microscopy was performed and assessment of tissue in inflammation, and the presence of pathological reparative changes their character. Results. The use of high doses of fentanyl for pain relief in animals can lead to hyperalgesia, accompanied by morphological changes in the skin after administration karaheninu. At 3:00 after driving fentanyl observed following morphological changes — the zone of damage was widespread, with no clear boundaries, with the development in the center of necrosis (thickness necrotic tissue was 5 mm) with severe perifocal reactive changes as significant inflammation (zone reactive changes thickness 7 mm), there have been serious violations of microcirculation. Conclusions. The use of ketamine for pain relief in these tissues were determined morphological changes after administration of fentanyl, but they were less expressive.

hyperalgesia, carrageenan, edema, analgesia

• 1. Кучин Ю. Л. Опіоїд-індукована гіпералгезія під час анальгоседації у пацієнтів з черепно-мозковою травмою / Ю. Л. Кучин, Ф. С. Глумчер, К. Ю. Бєлка // Біль, знеболювання та інтенсивна терапія. — 2012. — № 1 (Д). — С. 254-257.
• 2. Кучин Ю. Л. Стрес-індукована гіпералгезія у пацієнтів з множинною травмою / Ю. Л. Кучин // Біль, знеболювання та інтенсивна терапія. — 2013. — № 2 (Д). — С. 262-266.
• 3. Лесной И. И. Предупреждение развития хронического послеоперационного синдрома у хирургических больных / И. И. Лесной, В. И. Черний, К. Ю. Белка // Український журнал екстремальної медицини ім. Г. О. Можаєва. — 2012. — Т. 13, № 3. — С. 26-32.
• 4. Стефанов О. В. Доклінічні дослідження лікарських засобів / О. В. Стефанов. — Київ, 2001. — С. 77-95.
• 5. Angst M. S. Opioid-induced hyperalgesia / M. S. Angst, J. D. Clark // Anesthesiology. — 2006. — Vol. 104. — P. 570-587.
• 6. Lavand'homme P. K. Intraoperative epidural analgesia combined with ketamine provides effective preventive analgesia in patients undergoing major digestive surgery / P. Lavand'homme, M. D. Kock, H. Waterloos // Anesthesiology. — 2005. — Vol. 103 (4). — P. 813-820.
• 7. Wilder-Smith O. H. Postoperative hyperalgesia: its clinical importance and relevance / O. H. Wilder-Smith, L. Arendt-Nielsen // Anesthesiology. — 2006. — Vol. 104 (3). — P. 601-607.

«Bulletin of problems biology and medicine» Issue 1 part 1 (126), 2016 year, 277-280 pages, index UDK 615.035.4