DETERMINATION OF EUGENOL ESSENTIAL OIL MINIMUM INHIBITORY CONCENTRATION FOR FUNGI CULTURE ASPERGILLUS FUMIGATUS

Polianska V. P., Kinash O. V., Kovalenko N. P., Sargosh O. D.

MICROBIOLOGY

Scentific article

Recently, most researchers focus attention on possibilities of development and application of ecologically clean technologies and biological methods of pathogenic microorganisms control. The use of phytopreparations and essential oils is prospective direction in this respect. Essential oils, that are the natural concentrates of phytoncids, represent a multi-component organic aldehydes compounds (citronellal, citral), phenols (thymol, eugenol, carvacrol, anethole, eucalyptole), monoterpenic alcohols, ketones and other hydrocarbons. Most of the chemical compounds comprising essential oils, condition the antimicrobial, antibacterial, antiviral, anti-inflammatory, immune modulating properties. However, antifungal effect of eugenol on filamentous fungi is underinvestigated. Among filamentous fungi, the representatives of genus Aspergillus (A. fumigatus, A. flavus, A. niger) focus particular attention. The aim of the study was to determine the minimum fungistatic and fungicidal concentration of eugenol essential oil for the fungi culture of species A. fumigatus. According to obtained results, growth inhibition of fungi species Aspergillus fumigatus was observed in nutrient medium with concentration of eugenol essential oil, ranging 267,4 mg/ml – 8,35 mg/ml after incubation for 24 hours and 72 hours and minimum inhibitory eugenol concentration for Aspergillus fumigatus was 8,35 mg/ml. It is known, that eugenol is represented by lipophilic molecules that can penetrate between acyl chains of fatty acids, thereby affecting fluidity and permeability of membranes. This influences on the regulation and function of membrane-associated enzymes, that catalyze synthesis of the main polysaccharidic components of cell wall, including beta-glycan, chitin, mannan, which leads to cell growth disorder and membrane morphogenesis. When analyzing findings, it was stated that the minimum fungicidal concentration of eugenol essential oil in 24 hours and 72 hours of incubation is 8,35 mg/ml. Thus, fungistatic effect of eugenol on fungi species Aspergillus fumigatus according to these dilutions was not observed. Conclusions 1. Eugenol essential oil has strong fungicidal activity towards the fungi cultures of species Aspergillus fumigatus. 2. Minimum inhibitory concentration of the eugenol essential oil in 24 and 72 hours of incubation amounted to 8,35 mg/ml. 3. The minimum fungicidal concentration of eugenol essential oil amounted to 8,35 mg/ml. 4. Elongation of eugenol oil effect exposure on the cultures of fungi species Aspergillus fumigatus did not cause the growth of its fungicidal activity. 5. The bacteriostatic activity of eugenol essential oil towards the fungi culture species Aspergillus fumigatus was not revealed.

essential oil, eugenol, fungi culture Аspergillus fumigatus, minimum inhibitory concentration, minimum fungistatic concentration, minimum fungicidal concentration

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