HERPES VIRUS INFECTION IN CONDITIONS OF STROKE AND PHARMACOLOGICAL CORRECTION IN EXPERIMENT

Gumenyuk A. V., Rybalko S. L., Savosko S. I., Porva Yu. I., Chaikovsky Yu. B.

CLINICAL AND EXPERIMENTAL MEDICINE

Scentific article

Herpes is one of the most common infections in the population. Today problem is especially focused on persistent infections at nervous system injuries and immunodeficiency. However, in modern literature there are no data regarding the role of herpes infections as secondary complications of stroke, nor analyzed possibilities of pharmacological correction of persistent infection. The aim of the study was to establish the role of HSV-I type in severity of neurodystrophic process in model of local stroke and the possibility of its correction. We injected viral material (0,03 ml, 1-10 LD50 mouse lethal doses) in mouse brain for simulation of meningoencephalitis. The inflammation was registered in brain envelopes and tissues. The symptoms of infection were recorded on 5-6-th day after infection and reached the maximum to 13-14 days, and then the sings of infection decreased, the animals recovered. Since that time, we considered that HSV-1 was converted in latent form. This model is 100% reproducibility, useful for assessing severity of symptoms, and does not require additional methods of control. Hemorrhagic stroke and application of antiviral medications (acyclovir, altabor, neoflazid) were modeled in animals at 30-40 days after complete recovery. Experimental groups were: intact animals, animals with stroke, animals with HSV-1, animals with HSV-1 and stroke. Assessment of brain damage and influence of medications were performed by histological methods, PCR, ELISA and cell culture using (Vero cell line). Increased level of neurodystrophic violations in the hippocampus of infected animals after stroke was confirmed by histological method, and the level of HSV-1 reproduction was indicated by molecular biological methods in samples of blood serum and homogenates of brain tissue. The histological markers of herpes infection were: microglial density, focal infiltration of macrophages/microgliocites mainly along microvessels and ependymal cells in the brain ventricles; neuronal and glial cell pathology: pyknosis, apoptosis, multinucleolar neurons. Morphological manifestations of acute disruption of cerebral hemodynamics were swelling of brain tissue, hydropic cellular degeneration, angionecrosis and perivascular edema. Different manifestations of histological changes allowed to differentiate ischemic and infectious lesions, establishing the role of HSV-1 in severity of neurodystrophic process. Degree of cells injury was less pronounced in experimental groups with application of antiviral medications in stroke and latent HSV-1 infection. Reduce of apoptotic neuronal density due to acyclovir and altabor application was indicated in hippocampalCA1 area, neuronal hydropic dystrophy in acyclovir and neoflazid injection. Experimental data indicated the potential role of antiviral drugs in order to prevent the activation of persistent infection after stroke.

stroke, herpes simplex virus, antiviral medications

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«Bulletin of problems biology and medicine» Issue 2 part 1 (128), 2016 year, 41-45 pages, index UDK 616.831-005.1-022.6-06