KINETICS OF DEVELOPMENT OF MITOCHONDRIA AND DYNAMICS OF THE NUMBER OF MITOCHONDRIA IN CARDIOMYOCYTES OF THE COMPLEX (LV+ISH) IN EARLY POSTNATAL RAT ONTOGENESIS WISTAR

Zahoruiko G. E., Martsinovsky V. P., Zahoruiko Yu. V., Filatova V. L., Shmulich О. V.

BIOLOGY

Scentific article

It has been established that by 15 days after birth, the rat in the myocardium of the complex left ventricle and interventricular septum (LV + ISH) complete the proliferation and polyploidy of CMC. The number of CMC workers in the myocardium (LV + ISH) stabilizes at the level of 1,52 ...1,56 x 107 . In the myocardial parenchyma of newborn rat three populations of muscle cells are unequal in number and function: 1nuclear d-CMC, 1nuclear s-CMC and 2nuclear-CMCs. At t ≤ 15 days, the number of populations of 1nd- and 1ns-CMC decreases to “0” as result of the transition 1nd-CMC →1ns-CMC → 2n-CMC. In the process of postnatal development of rat pups, in the myocardial parenchyma (LV + ISH) a continuous increase in the sizes of 1nd-, 1ns-, 2n-CMCs and an increase in the volume of mitochondrioma in 1nd-, 1ns-, 2n-CMC is determined. The postnatal development of mitochondrioma in CMC is carried out through the implementation of the biological law “division ↔ merger”. In the time interval (n/p – 15), the division of MX and the increase in the number of these organelles in CMC are determined. The frequency of MX divisions in 1nd-CMC is 111 mx/day; in 1ns-CMC – 173 mx/day, in the 2n- CMC – 285 mx/day. The frequency of MX divisions in 2n-CMC equal to 267 mx/day, is determined in the time interval (30 – 45) days of postnatal development of rats. In the time interval (15 – 25) days in the 2n-CMC, an intensive fusion of the MX occurs with a frequency of 420 mx/day. The fusion of MX leads to an increase in the volume of organelles and a 2-fold increase in the content of MX DNA molecules in MX. The doubled amount of MX-DNA in MX promotes: intensification of biosynthesis and accumulation of MX-proteins in the MX matrix; an increase in the size of the surface area of cristae which acquire a convoluted and spiral shape; an increase in the volume of MA, the development of physiological hypertrophy of the MX in CMC. During postnatal ontogenesis in populations of CMC of the myocardium complex (LV + ISH), MX sizes increase from a minimum of 0.08 μm3 in 1nd-CMC of newborn rat to a maximum of 0.9 μm3 in 2n-CMC of 30- day- old rat.

cardiomyogenesis, populations of cardiomyocytes, mitochondrial division and fusion, mitochondrial apparatus.

1. Kennedy EP, Lehninger AL. Oxidation of fatty acids and tricarboxylic acid cycle intermediates by isolated rat liver mitochondria. J. Biol. Chem. 1949 Jun;179(2):957-72.
2. Lehninger AL. The Mitochondrion. New York, 1964. 160 p.
3. Rydın D, Yılkı D. Bıogenez mıtohondrıı. M.: Mır; 1970. 156 s. [in Russian].
4. Naryjnaıa NV, Maslov LN, Lıshmanov IyB. Pora, ızmenıaıýaıa pronıtsaemost mıtohondrıı – regylıator ystoıchıvostı serdtsa k deıstvııy reperfyzıı. Russian J. of Physiol. 2018;104(3):272-90. [in Russian].
5. Ong S-B, Hausenloy DJ. Mitochondrial morphology and cardiovascular disease. J. Cardiovasc. Res. 2010;88:16-29.
6. Hacker G. The morphology of apoptosis. Cell Tissue Res. 2000;90(13):5-17.
7. Myradıan HK. Apoptoz i starenie. Probl. stareniia ı dolgoletiia. 1999;8(1):85-102. [in Russian].
8. Patryshev MV, Mazýnın IO, Vınogradova EN. Slııanıe ı delenıe mıtohondrıı. Obzor. Bıohımııa. 2015;80(11):1745-54. [in Russian].
9. Hollander JM, Thapa D, Shepherd DL. Physiological and structural differences in spatially distinct subpopulations of cardiac mitochondria: influence of cardiac pathologies. Amer. J. Physiol. Heart Circ. Physiol. 2014;307:1-14.
10. Shponka IS. Gıstogenetıcheskıe protsessy v razvıvaıjemsıa mıokarde mlekopıtaıyıh. Dnepropetrovsk: POROGI; 1996. 228 s. [in Russian].
11. Zahoruyko GE, Zahoruyko YuV. Vozrastnye izmeneniya razmerov i chisla kardiomiocitov, ix yader v processe prenatalnogo i rannego postnatalnogo razvitiya serdca krys. Visnik probl. biol. i med. 2017;3(141):304-11. [in Russian].
12. Shmıdt-Nıelsen. Razmery jıvotnyh: pochemy oni tak vajny? M.: Mır; 1987. 259 s. [in Russian].
13. Avtandilov GG. Osnovy kolichestvennoy patologicheskoy anatomii. M.: «Meditsina»; 2002. 240 s. [in Russian].
14. Zahoruyko YuV, Zahoruyko GE, Martsınovkii VP, Filatova VL. Zakonomernostı kardıomıogeneza y krys Wistar: rost symmarnoı chıslennosti kardiomiotsitov i obrazovanie popyliatsii dvyiadernyh miotsitov v parenhime miokarda kompleksa (LJ +MJP). Visnik probl. biol. i med. 2019;2(149):70-5. [in Russian].

«Bulletin of problems biology and medicine» Issue 1 (155), 2020 year, 67-72 pages, index UDK 612.172 : 611.127-018

10.29254/2077-4214-2020-1-155-67-72