MONITORING OF ROTAVIRUS INFECTION IN THE POPULATION OF PAVLOGRAD

Govoruha O. Yu., Turitska T. G., Sidorenko G. G.

HYGIENE AND ECOLOGY

Scentific article

An epidemiological evaluation of the spread of rotavirus among children in Pavlograd was conducted. The studies were performed on the basis of Pavlograd City Hospital No. 4 of DOR. The study included 56 children with acute gastroenteritis between the ages of 2 and 11 who attended preschools and schools. The material for virological examination were the faeces of patients. For the detection of rotaviruses in faeces, we used the one-step immunochromatographic test “RotStick” (Novamed Ltd, Israel) and the “DRG Rotavirus Antigen (EIA-3509)” test system (DRG International Inc., USA). The results of the analysis were recorded and interpreted in accordance with the recommendations and formulas given in the guidance materials for the test kit. The dynamics of the titer of antibodies in the dynamics were evaluated by the result of the analysis in optical density units. The measurements were performed on a Spark microplate reader (Tecan, Switzerland). Multiplicity of examination of children ranged from 1 to 8 times depending on the length of their stay in the hospital. The incidence of rotavirus infection in children was found to be 46,4%, well above the 2019 average of 16,8%. In the current studies and in the retrospective analysis, the most vulnerable group of children were children between the ages of 2 and 5 years, where the frequency of rotavirus detection was 67,9% and in 2019 – 51,0%. Diseases were mainly recorded in winter: the incidence of rotavirus infection was 53,1% in the group of children.

rotavirus infection, gastroenteritis, enzyme immunoassay, frequency, seasonality

1. Tkhakushinova NKh. Rotavirusnaya infektsiya u detey. Epidemiologiya i infektsionnyye bolezni. 2012;2:56-9. [in Russian].
2. Crawford SE, Ramani S, Tate JE, Parashar UD, Svensson L, Hagbom M, et al. Rotavirus infection. Nat Rev Dis Primers. 2017;3:17083. DOI: 10.1038/nrdp.2017.83
3. Dennehy PH. Rotavirus Infection: A Disease of the Past? Infect Dis Clin North Am. 2015;29(4):617-35. DOI: 10.1016/j.idc.2015.07.002
4. Mayanskiy NA, Mayanskiy AN, Kulichenko TV. Rotavirusnaya infektsiya: epidemiologiya, patologiya, vaktsinoprofilaktika. Vestnik RAMN. 2015;1:47-55. [in Russian].
5. Kang HN, Park HK, Lee HJ, Moon JH, Oh JW, Kim CR. Rotavirus infection as a frequent cause of neonatal fever. Pediatr Int. 2018;60(4):366-71. DOI: 10.1111/ped.13504
6. Dzjublyk IV, Shun’ko JeJe, Kostenko OO, Kovaljuk OV, Obertyns’ka OV, Tunda IP, Bobyr VV. Rotavirusna infekcija: osoblyvosti laboratornoi’ diagnostyky, klinichnogo perebigu ta profilaktyky u novonarodzhenyh. Ukrai’ns’kyj medychnyj chasopys. 2007;3(59):5-6. [in Ukrainian].
7. Bulut Y, Yenişehirli G, Durmaz R. Molecular Epidemiology of Rotavirus Strains in Under Five Children. Indian J Pediatr. 2018;85(5):364-8. DOI: 10.1007/s12098-017-2540-9
8. Zvizdić S, Telalbasić S, Beslagić E, Cavaljuga S, Maglajlić J, Zvizdić A, Hamzić S. Clinical characteristics of rotaviruses disease. Bosn J Basic Med Sci. 2004;4(2):22-4.
9. Dzjublyk IV, Kovaljuk OV, Obertyns’ka OV, Kostenko OO. Zastosuvannja shvydkyh imunohromatografichnyh testiv v diagnostyci rotavirusnoi’ infekcii’ u ditej v specializovanyh dytjachyh likuval’nyh zakladah. Informacijnyj lyst. 2005;247. Kyi’v. [in Ukrainian].
10. Dobrjans’kyj DO. Strategija profilaktyky nozokomial’nyh infekcij u viddilennjah intensyvnoi’ terapii’ novonarodzhenyh. Mater. nauk. konf. «Perynatal’ni infekcii’ – suchasnyj pogljad na problemu». Kyi’v; 1999. s. 90-2. [in Ukrainian].
11. Soriano-Gabarro M, Mrukowicz-Vesikari J, Burden T. Оf rotavirus disease in European Union countries. J. Pediatr. Infect. Disease. 2006;25(1):7- 11.
12. Vesikari T, Rautanen T, Von Bondsdorff CH. Rotavirus gastroenteritis in Finland: burden of disease and epidemiological features. Acta Paediatr Suppl. 2009;98:24-30.
13. Chernyshova LI. Problema rotavirusnoi’ diarei’ u ditej. Sovremennaja pedyatryja. 2011;1(35):1-4. [in Ukrainian].
14. Velázquez FR. Rotavirus infections in infants as protection against subsequent infections. N. Engl. J. Med. 2007;12:208-13.
15. Shherbakov IT. Rotavirusnaja infekucija u detej. Arhiv patologii. 2000;3:12-25. [in Russian].
16. Dzjublyk IV. Rotavirusna infekcija u ditej: osoblyvosti etiologii’, patoimunogenezu ta epidemiologii’. K.: Vashe zdorov’ja; 2004. 41 s. [in Ukrainian].
17. Kljuchareva AA, Raevnev AE, Maljavko DV. Rotavirusnaja infekcija u detej. M.: Medicina; 2002. 237 s. [in Russian].
18. Parashar UD, Gibson CJ, Bresee JS, Glass RI. Rotavirus and severе childhood diarrhea. Emer. Infect. Diseases. 2006;12(2):304-6.
19. Vesikari T. The worldwide burden from rotavirus disease. European Rotavirus Journal. 2005;1:4-7.
20. Patel MM, Pitzer VE, Alonso WJ, Vera D, Lopman B, Tate J, et al. Global seasonality of rotavirus disease. Pediatr Infect Dis J. 2013;32(4):134-47. DOI: 10.1097/INF.0b013e31827d3b68
21. Parashar UD. Rotaviruses. Emerg. Infect. Dis. 2014;34:561-70.
22. Slobodkin VI, Dzjublyk IV, Trohymenko OP. Profilaktyka rotavirusnyh infekcij, pov’jazanyh z harchovym chynnykom peredachi zbudnykiv. K.: Vashe zdorov’ja; 2005. s. 15-8. [in Ukrainian].
23. Parashar UD, Nelson EA, Kang G. Diagnosis, management, and prevention of rotavirus gastroenteritis in children. BMJ. 2013;347:f7204. DOI: 10.1136/bmj.f7204

«Bulletin of problems biology and medicine» Issue 1 (155), 2020 year, 81-85 pages, index UDK 616.98:578.823.91]-053

10.29254/2077-4214-2020-1-155-81-85