THE EFFECT OF VARIOUS CONCENTRATIONS OF PECTIN ON ADHESIVE PROPERTIES OF CLINICAL ISOLATES OBTAINED FROM PATIENTS WITH CYSTIC FIBROSIS

Ishchenko O. V., Plotnikova M. M., Stoletova Yu. Yu., Sharun О. V., Stepanskyi D. O.

CLINICAL AND EXPERIMENTAL MEDICINE

Scentific article

Cystic fibrosis is a genetic disorder with an autosomal recessive type of inheritance. Chronic bronchopulmonary infection is the main cause of morbidity and mortality among such patients. Chronic infection is associated with the formation of a biofilm by microorganisms, especially Pseudomonas aeruginosa and Staphylococcus aureus. Biofilm-formation starts with adhesion of microorganisms to biotic or abiotic surfaces. The research objective was to study the effect of various concentrations of pectin on adhesive properties of clinical isolates of P. aeruginosa and S. aureus obtained from patients with Cystic Fibrosis. Methods. We used bacteriological, biochemical, microscopic methods. We performed the study of adhesive properties by the method of Brilis V.I. and co-authors with using of commercially available ram erythrocytes. The following indices were determined: the average adhesion index when counting at least 25 erythrocytes, including no more than five of them in one field of view; the participation rate of erythrocytes; microbial adhesive index, taking into account only the erythrocytes involved in the adhesive process. Results. Clinical isolates from patients with Cystic Fibrosis had high and medium ability to adhere. After incubation in 1% pectin solution, we observed a tendency to decrease in adhesiveness. The change in the average adhesion index under the influence of solutions with two different pectin’s concentrations was evaluated After incubation in 5% pectin solution we noted a significant decrease in the adhesiveness index of S. aureus and P. aeruginosa. A strong correlation was found between 5% pectin solution and a decrease in the average adhesion index. The correlation coefficient was -0,82 (p = 0,042) for S. aureus and -0,70 (p = 0,036) for P. aeruginosa. Conclusions. The use of a 5% pectin solution reduces the ability to adhere to clinical isolates isolated from patients with cystic fibrosis. We consider it is advisable to continue these experiments and to evaluate the effect of different concentrations of pectin on biofilm formation in a stationary model in vitro. In addition, it would be interesting to investigate the sensitivity to antibiotics of these microorganisms under the action of pectin.

Cystic Fibrosis, pectin, adhesiveness, P. aeruginosa, S. aureus.

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«Bulletin of problems biology and medicine» Issue 1 (155), 2020 year, 137-140 pages, index UDK 616-095: 579.61: 579.861.2: 57.017.3: 57.047

10.29254/2077-4214-2020-1-155-137-140