DETERMINATION OF THE MODIFYING INFLUENCE OF METAL CITRATES ON THE EMBRYOTIC TOXICITY OF CADMY IN RATES
About the author:
Shatorna V. F., Garets V. I., Baibakov V. M., Kononova I. I., Slesarenko O. G., Shamelashvili K. L.
Type of article:
The change in the environment of the industrialized countries prompts an intensive study of the impact of environmental factors on biological objects. The growth of urbanization inevitably leads to the complication of environmental conditions in cities, in the areas occupied by industrial enterprises, transport highways, as well as in the adjacent territories. In many countries, the priority group of eco-toxicants are heavy metals and, in particular, cadmium that reacts with superphosphate and is easily digested by plants in fairly large quantities.Thus, the problematic direction of morphological experimental research is the search for new possible bioanatogonists of toxicity of cadmium compounds among microelements that are capable of influencing dyslementosis, supporting the homeostasis of the organism, including during pregnancy. The experiment used cadmium chloride end citrate cerium. The purpose of the study was to experimentally determine the modifying effect of citrate cerium on the embryotoxicity of cadmium chloride when administered intragastric to rats. Object and methods. To simulate the effects and toxic effects of exposure to cadmium, throughout the pregnancy, Wistar’s female rats received cadmium chloride (1.0 mg/kg) each rat daily through the probe. In the second experimental group, the combined administration of cadmium chloride (1.0 mg/kg) and citrate cerium (1,3 μg/kg). The probable negative effect of the test substance on embryonic development was judged by the ability to increase the level of embryonic mortality (embryoal effect) and cause external and structural defects in the development of internal organs (teratogenic effect). The overall development of embryos was evaluated by the number of embryos, the number of yellow fetuses in the ovaries of females, the body mass of the embryo, and its compliance with the developmental stage according to generally accepted criteria for embryonic development of rats. Results and discussion. Comparison of the results of the embryotropic action of low doses of cadmium chloride with the parameters of the control group revealed its embryotoxicity, with practically the same number of yellow bodies of pregnancy (10.25 ± 0.27) in the group of exposure to cadmium chloride there is a significant (p <0.05) decrease in the number of live embryos by 11.7% on the 13th day and by 17.3% on the 20th day of embryogenesis. When combined with the introduction of cadmium chloride and cerium citrates, the decrease in the number of embryos in comparison with the control values was significantly lower: Such data indicate a decrease in the level of embryotoxicity of cadmium chloride by citrate cerium when combined with the introduction into the experiment in rats. The calculation of the indicators of general embryonic mortality revealed that the highest level of this indicator was determined on the 20th day of embryogenesis in the group of isolated effects of cadmium chloride and was 0,25 ± 0,02, which was 6.25 times greater than control. This situation was due to an increase in both preimplantation and post implantation mortality in the experimental group. Conclusions. The rates of embryonic development were better when combined with cadmium chloride with citrate cerium than the cadmium intoxication group. It indicates the modifying effect of the citrate cerium on the embryotoxicity of cadmium chloride.
cadmium chloride, citrate cerium, embryogenesis, embryotoxicity, embryonic mortality, rats.
- Fomenko OZ, Shaulskaya OE, Kot YG, Ushakova GA, Shevtsova AI. Vliyaniye raznykh doz kadmiya na aktivnost’ matriksnykh metalloproteinaz v serdtse, mozge i syvorotke krovi krys. Zhurnal Grodnenskogo gosudarstvennogo meditsinskogo universiteta. 2016;3:103-7. [in Russian].
- Batariova A, Spevackova V, Benes B, Cejchanovaa M, Smıda J, Cerna M. Blood and urine levels of Pb, Cd and Hg in the general population of the Czech Republic and proposed reference values. Int. J. Hyg. Environ. Health [Internet]. 2006 [cited 2018 July 17];209(4):359-66.
- Piasek M, Laskey JW, Kostial K, Blanusa M. Assessment of steroid disruption using cultures of whole ovary and/or placenta in rat and in human placental tissue. Arch Occup Environ Health. 2002;75:36-44. DOI: 10.1007/s00420-002-0351-3
- Piasek M, Laskey JW. Effects of in vitro cadmium exposure on ovarian steroidogenesis in rats. J Appl Toxicol. 1999 May-Jun;19(3):211-7.
- Salomeyna NV, Mashak SV. Strukturnye osnovy materynsko-plodovykh otnoshenyy pry khymycheskom vozdeystvyy v embryogeneze. Medytsyna y obrazovanye v Sybyry. 2012;1:123-36. [in Russian].
- Senkevych OA, Syrotyna ZV, Kovalskyy YuG, Berdnykov NV. Toksychnye mykroelementy plodovo-materynskogo kompleksa v uslovyyakh antropogennoy nagruzky. Dalnevostochnyy med. zhurn. 2008;2:61-4. [in Russian].
- Spivak YA, Nosenko ND, Zholobak NM, Shcherbakov AB, Reznikov AG, Ivanova OS, i dr. Nanokristallicheskiy dioksid tseriya povyshayet funktsional’nuyu aktivnost’ reproduktivnoy sistemy stareyushchikh samtsov krys. Nanosistemy: fizika, khimiya, matematika. 2013;4(1):72-7. [in Russian].
- Shatorna VF, Haretsʹ VI, Kononova II. Porivnyannya efektiv vplyvu soley svyntsyu ta kadmiyu na embriohenez u shchuriv. Ukrayinsʹkyy zhurnal medytsyny, biolohiyi ta sportu. 2018;3.6(15):310-4. [in Ukrainian].
- Nefʹodova OO, Halʹperin OI, Shatorna VF. Vplyv tsytrativ tseriyu ta hermaniyu na khid embriohenezu shchuriv na tli kadmiyevoyi intoksykatsiyi. Visnyk problem biolohiyi i medytsyny. 2019;1.1(148):61-6. [in Ukrainian].
- Dinerman AA. Rol’ zagryazniteley okruzhayushchey sredy v narushenii embrional’nogo razvitiya. M.: Meditsina; 1980. 191 s. [in Russian].
Publication of the article:
«Bulletin of problems biology and medicine» Issue 1 (155), 2020 year, 316-320 pages, index UDK 611.12-034:591.33-092.9