INFLUENCE OF DIFFERENT POLYMER CONCENTRATIONS ON THE STRUCTURE OF MICROFIBERS IN THE FIBROUS MATRIX

Pantus A. V., Rozhko M. M., Kovalchuk N. E., Yarmoshuk I. R.

METHODS AND METHODOLOGIES

Scentific article

Abstract. Today, the problem of restoration or replacement of tissues and organs using bio-scaffolds remains an urgent issue. When creating a tissue-engineered implant, it is important to provide matrix materials with a complex three-dimensional fibrous scaffold structure (non-woven scaffold) with a high ratio of surface area to total volume, imitating the intercellular tissue matrix. The currently existing promising method for synthesizing a fibrous matrix is electrospinning, which is energy-intensive and, accordingly, expensive. Currently, the development of a cheaper and cleaner method for the synthesis of fibrous matrices remains relevant. The aim of our study was to assess the nature of the structure of synthesized micro-flocks at various polymer concentrations. To carry out the research, we used a fibrous matrix obtained by our developed rotational synthesis from granules of 100% pure polycaprolactone (PCL) and polyllactide (PLLA). The resulting fibrous matrix was analyzed under a Leica DME light microscope. So, according to the results of the analysis of the thickness and microstructure of microfibers of thermoplastic biopolymers PCL and PLLA, it can be concluded that at very low initial concentrations of polymers in the sucrose melt, microfibers with the smallest transverse diameter prevailed in the fibrous matrices, but in the form of thin welded and twisted fragmented threads with available globules on their surface. This effect can be explained by the fact that during the synthesis of microfibers at low polymer concentrations and uneven stretching leads to twisting, adhesion and soldering to each other. With an increase in the polymer concentration, its volume in the sucrose melt, on the contrary, made it possible to fully stretch the fiber, which led to an increase in the number of microfibers more organized in the form of thin and thick microfibers in the matrix.

: biopolymer, matrix, microfiber, sucrose.

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«Bulletin of problems biology and medicine» Issue 1 (159), 2021 year, 174-176 pages, index UDK 616-089.843+615.461+576.31

10.29254/2077-4214-2021-1-159-174-176