INFLUENCE OF ISOCHINOLINE ALKALOID EXTRACT OF POPPY PLANTS ON BIOLOGICAL PROPERTIES OF MUSEUM STRAINS OF MICROORGANISMS

Derevianko T. V., Ananieva M. M., Faustova M. O., Loban’ G. A.

MICROBIOLOGY

Scentific article

Abstract. The results of the study of antimicrobial activity of the phytopreparation Sangviritrin, which is an alcoholic extract from plants of the poppy family – Macleaya cordata and Macleaya microcarpa, were analyzed for reference strains of microorganisms Staphylococcus aureus ATCC 25923, Escherichia coli ATC, Candida albicans ATCC 10231. The antimicrobial action of the drug is due to isoquinoline alkaloids – sanguinarine and chelerythrin (in a ratio of 3:7). Determination of antibacterial and antifungal activity of the drug was performed by qualitative method of diffusion into agar (Kirby-Bauer) and quantitative macromethod of double serial dilutions in a liquid nutrient medium according to the order of the Ministry of Public Health of Ukraine №167 from 05.04.2007 «About the statement of methodical instructions «Determination of sensitivity of microorganisms to antibacterial drugs». To study the effectiveness of antimicrobial action of the drug, we studied its effect on the change of pathogenicity factors of the museum strain S. aureus by assessing plasma coagulation, lecithinase and hemolytic activity of microorganisms in the presence of plant extract by conventional methods. The aim was to study the effect of isoquinoline alkaloid extract of plants of the poppy family on the biological properties of museum strains of microorganisms. The different activity of the phytopreparation against museum strains of gram-positive and gram-negative microorganisms has been proved. The culture of Staphylococcus aureus was the most sensitive to the active substances of sanguinarine and chelerythrin. The drug showed significant fungicidal activity against the studied reference strain of C. albicans. Culture of Escherichia coli showed high resistance to isoquinoline alkaloids of the phytopreparation. The effect of the active substances of Sangviritrin on the change of virulence factors of Staphylococcus aureus, which loses plasma-coagulating properties, lecithinase and hemolytic activity under direct and long-term action, has been studied. The influence of biologically active substances of the phytopreparation on the change of tinctorial properties of gram-positive microorganisms (S. aureus and C. albicans) has been established.

sanguiritrin, phytopreparation, antibacterial action, antifungal action, sensitivity, resistance, pathogenicity factors.

«Bulletin of problems biology and medicine» Issue 1 (159), 2021 year, 181-184 pages, index UDK 578/579.063.8./083:615.451.1:582.675.5

10.29254/2077-4214-2021-1-159-181-184