NANOTUBS INCREASE OXIDATIVE AND NITROOXIDATIVE STRESS INDUCEDBY TETRACHLOROMETHANE

Летняк Н. Я., Корда М. М.

CLINICAL AND EXPERIMENTAL MEDICINE

Scentific article

Carbon nanoparticles, due to the wide use in many countries in different areas of industry, life and medicine, can be considered as a new global anthropogenic factor, which is characterized as a potential danger for human health. The capability of nanotubes to transport medicines and chemicals inside a cell predicts the possibility of the increase of classical substances toxicity in case of their intake into the body together with nanotubes. Objective.The aim of the research was to study the effect of carbon nanotubes on the capability of the chemical toxicant tetrachloromethane (TCM) to induce nitrooxidative stress and enhance the oxidative modification of proteins in serum and liver of rats. Methods.The experiments were performed on outbred male rats, which were administered intraperitoneally with 0.5 ml of suspension of single-walled, multi-walled or multi-walled functionalized by COOH nanotubes (60 mg/kg) separately or together with TCM (2 ml/kg). The animals were taken out of the experiment in 3, 6 and 48 hours after the administration of the nanotubes and TCM. In serum and liver the total activity of NO synthase, level of NOx and oxidative-modified proteins were measured. Results.It has been shown that only multi-walled carbon nanotubes changed significantly the studied parameters. The administration of tetrachloromethane to rats caused significant changes of all indices. Maximal changes of all parameters were registered in the group of animals that were co-administered with carbon nanotubes and tetrachloromethane. In this case, a number of the studied parameters in blood and liver significantly changed compared to the similar indicators in the group of animals, which were administered with the chemical toxicant only. Conclusion.Carbon nanotubes increase the capability of the chemical toxicant tetrachlormethane to cause nitrooxidative stress and increase the oxidative modification of proteins in liver and serum.

carbon nanotubes, tetrachloromethane, nitrooxidative and oxidative stress

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«Bulletin of problems biology and medicine» Issue 1 Part 1 (142), 2018 year, 140-144 pages, index UDK 612.015.11-02:616-099:546.26– 022.532] – 092.9

10.29254/2077-4214-2018-1-1-142-140-144