CARBOHYDRATE SPECIFICITY OF CELLULAR ELEMENTS OF THE RED BONE ERYTHROBLAST ISLET IN RATS WHEN MODELING EXPERIMENTAL INFLAMMATION

Bilash S. M., Boruta N. V., Starchenko I. I., Eroshenko G. A., Lysachenko O. D.

MORPHOLOGY

Scentific article

The purpose of the workwas to determine the receptors –glycopolymers of plant origin in the cellular elements of the erythroblast islet of a red bone marrow in rats when modeling an aseptic inflammation.Object and methods of the research. An experimental study was performed on 50 mongrel white rats. An acute aseptic inflammation was simulated in 45 animals by intraperitoneal injection of 5 mg of λ-carrageenan (“Sigma”, USA) in 1 ml of isotonic sodium chloride solution per 1 animal. The animals were withdrawn from the experiment on the 1st, 2nd, 3rd, 5th, 7th, 10th, 14th, 21st and 30th day by an overdose of thiopental anesthesia. The study of red bone marrow was carried out in accordance with the established terms of the experiment. A set of biological material for conducting research was conducted under conditions of a small operating vivarium of the HSEEU “Ukrainian Medical Stomatological Academy” in accordance with the “Rules for the Use of Laboratory Experimental Animals” (2006, Annex 4) and the Helsinki Declaration on the Humane Approach to Animals. For the study, preparations of red bone marrow in rats were used. After gathering, the material was fixed in a 10% solution of neutral formalin with subsequent decalcification in a solution of ethylenediaminetetraacetic acid, with keeping to ph 7.4. For gaining observation preparations, sections with thickness up to 5 micrometer were stained with hematoxylin and eosin. Subsequently, the preparations were treated using standard sets of NPK “Lektinoest” (Lviv) in the development of lectins 1:50 by the method. Results of the research and their discussion.In the study of histological preparations of the red bone marrow, it was found that the stroma of the hematopoietic organ was represented by trabecula of bone and reticulum tissue in which a large number of blood vessels were located, mainly sinusoidal capillaries without a basement membrane, but with pores in the endothelium. Parenchyma of the red bone marrow was represented by islets in which the programed differentiation of hemopoietic cells were detected at different stages of it. The erythroblastic islet of the red bone marrow is a structuralfunctional unit of the organ, and was presented by the cells of the erythroblast series, namely: proerythroblast, basophilic, polychromatophilic, and orthochromic erythroblasts, which were located around macrophages. The basis of the method of carbohydrate specificity study is the use of lectins, which allows to elaborate morphofunctional changes in the structural elements in rats under conditions of experimental inflammation, due to the binding of lectins to glucoconjugates that are on the cell surface. It is proved that carbohydrate residues on cellular elements of the erythroblast islet periodically change the intensity of accumulation of receptors to one or another lectin, determine the different functions of the same lectin or system of lectins.

experimental inflammation, erythroblast islet, carbohydrate residues, lectins

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«Bulletin of problems biology and medicine» Issue 1 Part 1 (142), 2018 year, 261-264 pages, index UDK 611.018.46:(612.08:599.323.4)

10.29254/2077-4214-2018-1-1-142-261-264