THE INFLUENCE OF DYNAMIC ELECTRONEUROSTIMULATION ON MORPHOMETRIC INDICES OF MUCOSA OF THE MAXILLARY SINUS OF EXPERIMENTAL ANIMAL WITH MODEL OF CHRONIC SINUSITIS

Сурин А. В., Походенько-Чудакова И. О., Герасимович А. И., Адольф Е. В.

MORPHOLOGY

Scentific article

Morphometry meets all the principles of evidence-based medicine and objectively confirms the orientation of the pathological process dynamics when those or other methods of treatment are applied. The purpose of the studywas to determine the influence of dynamic electroneurostimulation (DENS) on morphometric parameters of mucosa of the maxillary sinus of experimental animal model of chronic sinusitis. Object and methods. The study involved 22 male rabbit with the model of chronic sinusitis of the maxillary sinus and divided into two series. In series A (12) we studied the formation of chronic sinusitis of the maxillary sinus in a period of 3 months after the introduction into the sinus of the filling material for filling root canals of teeth. In series B (10) we studied the influence of DENS on the course of chronic sinusitis. The study included a descriptive morphology and morphometry with the calculation in the digitized fields of view of inflammatory cell infiltration (leucocytes, eosinophilic granulocytes, lymphocytes, plasma cells, macrophages, giant multinucleated cells) and the number of vessels and fibroblasts, reflecting the processes of reparative regeneration. Results. Morphological pattern and morphometric parameters of the series A show that the introduction into the maxillary sinus of the filling material stimulates the conditions for the inflammatory process with chronic undulating relapsing. In series B it was noted that after a course of DENS in most of examinations we found chronic productive inflammation going into a phase of decay with a predominance of processes of the organization and regeneration. There were no visible exudative reaction including microabscesses, there were only minor small focus admixture of neutrophils and eosinophilic granulocytes in a predominant mononuclear infiltrate. In the his to pathological picture the regenerative processes with a relatively rare appearance of fibroblasts, dominated signs of fibroplasia, some atrophy of the epithelium. Determined pronounced neoangiogenesis, represented by clusters of full-blooded venules, proliferating endotheliumand granulation tissue varying degrees of maturity, emboli, and aggregates of foreign material in the lymph and blood vessels. It was noted the formation of polyps with a predominance of macrophage giant cell granulomas over. Only in one field of view were identified coarse inclusions filling material, “immured” in a dense coarse-fibered connective tissue, indicating a more intense elimination of foreign material. Conclusion. Identified in the experiment the decrease in the activity of chronic inflammatory process with a clear predominance of productive tissue reactions and a significant decrease in exudation, decrease in the degree of its severity and duration, the predominance of the processes of the organization and reparation allows to achieve stable remission and therefore to conclude that the positive therapeutic effect of DENS on the course of chronic sinusitis of the maxillary sinus. Prospects for further research. Experimental results prove the feasibility of the application of DENS in complex postoperative treatment in the clinics.

experimental sinusitis, maxillary sinus, morphometry, reflexology, dynamic electroneurostimulation

1. Avtandilov GG. Medicinskaja morfometrija: rukovodstvo. M.: Meditsina; 1990. 384 s. [in Russian].
2. Amangaliev AB, Vansvanov MM, Zhakenova SS, Amangalieva GE, Ilyasov AM. Jendoskopicheskoe lechenie verhnecheljustnyh sinusitov. Bull. AGIUV. 2013;4:80-3. [in Russian].
3. Bubnova MG, Butina EK, Vygodin VA, Koltunov IE, Kukushkin SK, Kutishenko NP, i dr. Osnovy dokazatel’noj mediciny. Uchebnoe posobie dlja sistemy poslevuzovskogo i dopolnitel’nogo professional’nogo obrazovanija vrachej. M.: Silitseya-Polygraph; 2010. 136 s. [in Russian].
4. Volkov VP. Integral’naja statisticheskaja ocenka kolichestvennyh pokazatelej v morfologicheskih issledovanijah: korrigirovannyj algoritm [Internet]. Universum. Med. & Pharm.: Sci. e-J. 2016;28(6). Available from: http://7universum.com/ru/med/archive/item/3286 [in Russian].
5. Gadzhiev RS, Akhmadova MA, Gerasimenko MYu. Fljuktoforez meksidola u bol’nyh odontogennym verhnecheljustnym sinusitom. Fizioterapiya, bal’neologiya i reabilitatsiya. 2013;6:47-8.[in Russian].
6. Denisov SD, Morozkina TS. Trebovanija k nauchnomu jeksperimentu s ispol’zovaniem zhivotnyh. Zdravookhraneniye. 2001;4:40-2. [in Russian].
7. Edranov SS, Coj VG. Jeksperimental’naja travma kak model’ dlja issledovanija reparativnyh mehanizmov slizistoj obolochki verhnecheljustnogo sinusa. Tikhookeanskiy med. zhurnal. 2014;3:9-13. [in Russian].
8. Korzh AA, Belous AM, Pankov EJa. Reparativnaja regeneracija kosti. M.: Meditsina; 1972. 232 s. [in Russian].
9. Korotkich NG, Toboev GV. Morfometricheskoe issledovanie vospalitel’nogo infil’trata pri ostryh odontogennyh gnojno-vospalitel’nyh zabolevanijah lica. Vestnik Smolenskoy med. akademii. 2010;2:78-9. [in Russian].
10. Koshel’ IV. Rol’ sosudistyh narushenij v morfogeneze verhnecheljustnogo sinusita odontogennoj jetiologii. Med. vestnik Severnogo Kavkaza. 2017;12(1):91-4. DOI: 10.14300/mnnc.2017.12025 [in Russian].
11. Pohodenko-Chudakova IO, Kazakova YM. Refleksoterapija v kompleksnom lechenii odontogennyh abscessov cheljustno-licevoj oblasti. Minsk: BGU; 2011. 133 s. [in Russian].
12. Pohodenko-Chudakova IO, Surin AV, Gerasimovich AI. Sposob medelirovanija jeksperimental’nogo hronicheskogo sinusita verhnecheljustnoj pazuhi u krolika. Aficyjny biulieteń. Vynachodstva, karysnyja madeli, pramyslovyja ŭzory. 2017;5(118):131. [in Russian].
13. Rebrova OJu. Statisticheskij analiz medicinskih dannyh. Primenenie paketa prikladnyh programm STATISTICA. M.: Media sfera; 2002. 312 s. [in Russian].
14. Rjavkin SJu, Vlasov AA, Nikolaeva NB, Safronov AA, Umnikova MV. Prakticheskoe rukovodstvo po dinamicheskoj jelektronejrostimuljacii. Ekaterinburg: Tokmak-Press; 2011. 232 s. [in Russian].
15. Sipkin AM, Gerasimenko YuM, Nikitin AA, Hrykova AG, Fomina EV. Lazeroforez «Koleteks-AGGDM» v posleoperacionnoj reabilitacii bol’nyh verhnecheljustnym sinusitom. Fizioterapiya, bal’neologiya i reabilitatsiya. 2013;3:6-10.[in Russian].
16. Shchedrenok VV, Moguchaya OV, Zakhmatov IG, Potemkina EG, Simonova IA, Anikeev NV, i dr. Znachenie izmerenija anatomicheskogo intrakranial’nogo rezerva v nejrohirurgii [Internet]. Neyrokhirurgiya. 2014;15. Available from: http://www.medline.ru/public/pdf/15_039.pdf [in Russian].
17. Badarne O, Koudstaal MJ, van Elswijk JF, Wolvius EB. Odontogenic maxillary sinusitis based on overextension of root canal filling material. Ned. Tijdschr. Tandheelkd. 2012;119(10:480-3.
18. Burnham R, Bridle C. Aspergillosis of the maxillary sinus secondary to a foreign body (amalgam) in the maxillary antrum. Br. J. Oral Maxillofac. Surg. 2009;47(4):313-5. DOI: 10.1016/j.bjoms.2009.01.015
19. Dżaman K, Zagor M, Sarnowska E, Krzeski A, Kantor I. The correlation of TAS2R38 gene variants with higher risk for chronic rhinosinusitis in Polish patients. Otolaryngol. Pol. 2016;70(5):13-8. DOI: 10.5604/00306657.1209438
20. González-Díaz SN, Arias-Cruz A, Rivero-Arias DM, Partida-Ortega AB, Elizondo-Villarreal B, Ibarra-Chávez JA, et al. Cross-reactivity in allergic fungal sinusitis. Case report. Rev. Alerg. Mex.2016;63(3):316-9. DOI: 10.29262/ram.v63i3.166
21. Komar J, Morozov V. Die abhängigkeit der morphometrischen parameter des herzens der leichtathleten von der zielrichtung des trainingsprozesses (The dependence of the morphometric parameters of the heart of the athletes from the direction of the target of the training process). East Europ. Sci. J. 2017;20(4, part 1):85-8.
22. Lam EP, Kariyawasam HH, Rana BM, Durham SR, Mc Kenzie AN, Powell N, et al. IL-25/IL-33-responsive TH2 cells characterize nasal polyps with a default TH17 signature in nasal mucosa. J. Allergy Clin. Immunol. 2016;137(5):1514-24. DOI: 10.1016/j.jaci.2015.10.019
23. Mendiola M, Tharakan A, Chen M, Asempa T, Lane AP, Ramanathan MJr. Characterization of a novel high-dose ovalbumin-induced murine model of allergic sinonasal inflammation. Int. Forum Allergy Rhinol. 2016;9(6):964-72. DOI: 10.1002/alr.21768
24. Procacci P, Zangani A, Rossetto A, Rizzini A, Zanette G, Albanese M. Odontogenic orbital abscess: a case report and review of literature. Oral Maxillofac. Surg. 2017;21(2):271-9. DOI: 10.1007/s10006-017-0618-1
25. Red’ko DD, Shljaga ID. Fungal sinusitis. (Literature review). Problems of Health and Ecology. 2012;32(2):34-40.
26. Roux Ch. The living skeleton. Wolters Kluwer Health; 2007. 113 р.

«Bulletin of problems biology and medicine» Issue 1 Part 1 (142), 2018 year, 305-310 pages, index UDK 616. 216. 1 – 002. 2: 615. 8 – 092. 9

10.29254/2077-4214-2018-1-1-142-305-310