Kryzhna S. I., Kievskaya Yu. O., Tyupka T. I., Kozar V. V.

STUDY OF THE OXIDATIVE STRESS STATE DURING EXPERIMENTAL RHINITIES OF DIFFERENT GENESIS


About the author:

Kryzhna S. I., Kievskaya Yu. O., Tyupka T. I., Kozar V. V.

Heading:

CLINICAL AND EXPERIMENTAL MEDICINE

Type of article:

Scentific article

Annotation:

It has been proved that inflammatory cells release a number of reactive oxygen species at the site of inflammation, which leads to an increase in oxidative stress. On the other hand, a number of reactive oxygen / nitrogen species (as etiologic factors) can initiate an intracellular cascade of signaling that enhances the expression of an inflammatory gene. Thus, inflammation and oxidative stress are closely related pathophysiological phenomena. The aim of the work was to determine the degree of violation of lipid peroxidation and antioxidant protection at the local and system levels and, most importantly, on models of different types of rhinitis in terms of the coefficient of oxidative stress in conditions of experimental rhinitis. Practical significance of a pathophysiological researches for medical practice consists in theoretical synthesis and a new solution of the actual scientific and practical problem of modern biological and medical pathological physiology – the role of antioxidants in the pathogenesis of rhinitis of various genesis, which allows pathogenetically substantiate the principles of correction of this pathological process. The first model of acute inflammation of the nasal cavity was chemical rhinitis caused by caustic soda by introducing a damp swab saturated with 40% sodium hydroxide solution, in each nostril of the nose for 1-2 seconds. The experimental model of bacterial rhinitis was reproduced by intranasal single administration of the daily culture of the Staphylococcus aureus ATCC 25923 mutation (in each nasal passage) of 0.2 mm3. The rats were divided into 2 groups: the first group was intact control, the second – chemical or bacterial rhinitis. The observation time was 14 days. The lipid peroxidation / antioxidant protection parameters were studied in the intranasal washings and blood serum that were tested on the 3 rd and 14 th day of the experiment. One of the stages of the pathophysiological study of the state of the prooxidant-antioxidant state in terms of the oxidative stress of the nasal mucosa at the local and general levels in rats with chemical and bacterial rhinitis was carried out. Simulation of rhinitis in terms of the coefficient of oxidative stress reliably led to a high degree of disruption of the POL and AOD systems for various types of rhinitis (chemical and bacterial) on the third day at the local and systemic levels of pathology. It was proved that by the end of the observation period, the power of compensatory mechanisms was insufficient, which was manifested by a high oxidative stress coefficient for both types of rhinitis at the local and systemic levels. With chemical rhinitis at the system level, such shifts were more pronounced – 1.49 times on the third day and 1.27 times on the fourteenth day of the experiment. With bacterial – 2.0 times on the third day and 2.59 times on the fourteenth day of the experiment. The degree of violation of the POL and AOD was more pronounced with bacterial rhinitis at the system level compared with chemical rhinitis. The results obtained allow us to continue studies of the course of experimental rhinitis, the mechanisms of development of protection and tissue repair.

Tags:

rhinitis, oxidative stress ratio

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Publication of the article:

«Bulletin of problems biology and medicine» Issue 1 Part 1 (148), 2019 year, 133-137 pages, index UDK 616.211-002:678.048]-092.9

DOI: