SENSITIVITY TO ANTIBIOTICS OF CLINICAL ISOLATES OF STAPHYLOCOCCI

Nadverniuk R. A., Voronkova O. S., Frankenberg A. A., Shevchenko T. M.

MICROBIOLOGY

Scentific article

Staphylococcal infection, according to the World Health Organization, continues to spread everywhere with the growing dynamics. Diseases are found in all age groups of the population, starting with childhood. The uniqueness of these lesions is that they are able to affect all organs and systems in the form of localized or generalized infection. Priority in the problem of staphylococcal infections have the question of the spread of resistance to antibiotics among clinical isolates, which mediates the emergence of problems in the use of therapeutic measures. Staphylococci today are recognized as one of the most significant groups of microorganisms, which have a significant carriage of resistance determinants. The timely diagnosis of staphylococcal disease and the individual determination of the susceptibility of strains to antibiotics is the basis for rational therapeutic and prophylactic measures. The aim of the research was to determine the species spectrum and antibiotic susceptibility of isolated staphylococcal strains. The research was carried out on the basis of the bacteriological laboratory of the Tsarichanka Town Clinical Hospital. The object of the study was the frequency of detection and biological properties of clinical isolates of staphylococci. The subject of the study was the isolation of gram-positive cocci, a distinction from individuals with different types of lesions. Standard methods were used to isolate, identify and study of antibiotic susceptibility of isolates. In the study of 23 samples of biological material suspected on staphylococcal carriage, it was determined that it was present in 16 (69.6%) specimens. Species identification showed that in 11 cases (68.8%) S. aureus was present. Representatives of the S. epidermidis species were found in 4 (25.0%) cases and in 1 (6.2%) cases isolated strains was identified as Staphylococcus spp. The obtained range coincides with the known data that the leading role in human pathology belongs to the S. aureus, which can easily colonize the body and cause pathogenetic manifestations. For all isolated strains, the manifestation of plasmocoagulase, lipase, lecithinase and hemolytic activity was investigated. Thus, it was found that among of all isolated strains of staphylococci only 68.8% produced plasmacoagulase, 87.5% – were able to produce lipase and lecithinase, 93,8% – hemolysins. The most active antibiotic from the group of drugs acting on the synthesis of the cell wall, against all the examined strains of S. aureus was imipenem (carbapenem), to which all 11 (100%) strains were susceptible. Unlike amoxicillin, it was significantly more effective. In particular to the latter were sensitive to 18.2% of strains. Inadequate activity is determined for cefepime: only 4 strains (36.4%) were susceptible. The high sensitivity of the isolated S. aureus strains is determined by rifampicin – 9 strains are susceptible (81.8%) and gatifloxacin (90.9%). Antibiotics of the tetracycline and aminoglycoside groups were characterized by low efficacy against the isolated S. aureus strains – more over 60% of the strains were resistant. The speading of antibiotic resistance among clinical strains is becoming increasingly significant. Therefore, an essential element of the diagnostic procedures is the performance of the antibioticogram to determine the range of sensitivity to antibiotics in order to develop a scheme of rational antibiotic therapy of staphylococcal lesions. Systemic monitoring of the sensitivity of clinical isolates of staphylococci to antibiotics can be used to develop of schemes to prevent the spread of determinants of resistance among microorganisms.

staphylococci, clinical isolate, species spectrum, sensitivity to antibiotics

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«Bulletin of problems biology and medicine» Issue 1 Part 1 (148), 2019 year, 254-257 pages, index UDK 579.61

10.29254/2077-4214-2019-1-1-148-254-257