Markovskaya I. V., Sokolova I. I., Markovskaya E. V.

THE TOTAL PROTEIN CONTENT AND SEVERAL ENZYME ACTIVITY IN THE RAT ORAL FLUID UNDERGOING ELECTROMAGNETIC RADIATION


About the author:

Markovskaya I. V., Sokolova I. I., Markovskaya E. V.

Heading:

DENTISTRY

Type of article:

Scentific article

Annotation:

The electromagnetic radiation, performed by various electric field with low frequency 70 kHz, action results in the decrease of the functional state of salivary glands and the protective properties of the oral fluid to be reflected by changes of the major biochemical parameter content in the oral fluid and contributes to the development of periodontitis, caries and other pathological processes in the oral cavity. Aim: the total protein content, proteolytic, amylase, phosphatase and kallicrein activity determination in the rat oral fluid underdoing electromagnetic radiation. Results: the total protein content decrease by 31.68% on the background of the proteolytic activity increase by 41.53% in comparison with the control was determined the oral fluid. Amylase activity is decreased by 39.6%. A significant increase of the alkaline and acid phosphatase activity is observed by 28.98% and 79.0%, respectively in the oral cavity of rats. The conducted are carried out to show a decrease in the functional state of the salivary glands and protective properties of the oral fluid under conditions of electromagnetic radiation proved by a decrease of the total protein content, lysozyme amylase activity. The detected changes will lead to functional activity of protein molecules and protective properties of the oral fluid loosing. Defined hypoamylasemia is a consequence of reduced function of parotid glands, damage of secretory cells by products of vital activity of microorganisms. Increased alkaline and acid phosphatase activity contributes to the destruction of periodontal tissues, the release of enzymes from connective tissue cells and the reduction of reparative processes. The influence of electromagnetic radiation is also determined to show an activation of the kinin system that can initiate inflammatory processes in the oral cavity.

Tags:

electromagnetic radiation, oral fluid, total protein, proteolytic activity, enzymes

Bibliography:

  1. Bebeshko VG, Kovalenko AN, Belyy DA. Ostryy radiatsionnyy sindrom i yego posledstviya. Ternopol’: TGMU; 2006. 436 s. [in Russian].
  2. Brandtzaed P. Immunology of tonsils and adenoids: everything the ENT surgeon needs to know. Int. J. Pediatr. Otorhinolaryngol. 2003;67: 69-76.
  3. Kolesov SA, Korkotashvili LV. Proteom slyuny i yego diagnosticheskiye vozmozhnosti. Klinicheskaya laboratornaya diagnostika. 2015;5:54-8. [in Russian].
  4. Ruhl S. The scientific exploration of saliva in the post-proteomic era: from database back to basic function. Expert. Rev. Proteomics. 2012;9(1):85-96.
  5. Schultz BL, Cooper-White J, Punyadeera CK. Saliva proteome research: current status and future outlook. Crit. Rev. Biotechnol. 2013;33(3): 246-59.
  6. Markovsʹka IV, Sokolova II, Myroshnychenko MS. Sposib zaboru slyny u shchuriv. Patent na vynakhid. Byul. № 4 vid 25.07.2016. [in Ukrainian].
  7. Kolb VG, Kamyshnikov VS. Spravochnik po klinicheskoy khimii. Minsk: Belarus’; 1982. 366 s. [in Russian].
  8. Mamedaliyev NA, Divocha VA. Aktivnost’ proteoliticheskikh fermentov i ikh ingibitorov v rotovoy zhidkosti zdorovikh lyudey i patsiyentov s simptomami ostrykh respiratornykh virusnykh infektsiy. Medichna ta klíníchna khímíya. 2017;19-3:103-7. [in Russian].
  9. Ivanytsʹka HI, Lyulechko LV, Ivanytsʹka MV. Praktykum z klinichnoyi biokhimiyi: navch. posibnyk. Kyev: VSV «Medytsyna»; 2010. 184 s. [in Ukrainian].
  10. Paskhina TS, Krinskaya AV. Uproshchennyy metod opredeleniya kallikreinogena i kallikreina v syvorotke (plazme) krovi cheloveka v norme i pri nekotorykh patologicheskikh sostoyaniyakh. Voprosy meditsinskoy khimii. 1974;20.6:660-3. [in Russian].
  11. Kobzar’ AI. Prikladnaya matematicheskaya statistika. Moskva: FIZMATLIT; 2012. 816 s. [in Russian].

Publication of the article:

«Bulletin of problems biology and medicine» Issue 1 Part 1 (148), 2019 year, 340-343 pages, index UDK 616.311-008.843.1-001.2-092.9:57.088.6

DOI: