THE INFLUENCE OF VANADIUM CITRATE ON BODY MASS, BLOOD GLUCOSE LEVEL AND ANTIOXIDANT PROTECTION IN THE LIVER AND KIDNEYS OF RATS WITH ALLOXAN DIABETES MELLITUS
About the author:
Sushko O. O., Iskra R. Ya.
Type of article:
Today, diabetes mellitus is one of the most common metabolic diseases. Therefore, there is growing interest in finding new pharmacological agents for the prevention and treatment of diabetes mellitus. The aim of our studies was to find the influence of vanadium citrate on the body mass, blood glucose level and on the state of the pro/antioxidant system in the liver and kidneys of rats with alloxan-induced diabetes mellitus. Object and methods. The research was conducted on 40 white laboratory rats kept in the vivarium of the Institute of Animals Biology. Rats with the weight in the range of 100-120 g were divided into four groups: I – the control group, II – the control group with diabetes, III, IV, V – experimental groups. Rats from groups I and II were given pure water without any additives; III, IV and V were given water with the solution of vanadium citrate in the amounts of 0.125, 0.5 and 2.0 μg/mL of water. Experimental diabetes mellitus (EDM) was induced in the animals from II, III, IV and V after a 24-h fasting period by intraperitoneal administration of 5% solution of alloxan monohydrate (“Synbios”) in the amount of 150 mg/kg of body weight. In order to detect hyperglycemia, we collected blood from the tail vein and measured glucose level in the collected blood using a portable glucose meter (Gamma-M). Dynamics of changes in glucose levels was carried out immediately before the start of the experiment and was continued after the injection of alloxan on the 32nd, 36th, and 40th days of studies. Glucose level in rats` blood more than 11.1 mmol/L was accepted as a successful induction of diabetes mellitus. Normal healthy rats were injected with physiological saline. On day 40 of the study, the animals were withdrawn from the experiment and decapitated under thiopental sodium. We determined the content of lipid hydroperoxides, TBA-active products, the content of reduced glutathione, catalase activity, superoxide dismutase activity, glutathione peroxidase and glutathione reductase activities in liver and kidneys tissue. Results. The weight of the body of animals with diabetes decreased, and the glucose concentration increased in the blood of these animals. Body mass increased, and the blood glucose concentration decreased due to the effects of vanadium citrate in different doses compared to the level of rats with EDM from group II. The activity of catalase (CAT), superoxide dismutase (SOD), and glutathione levels of antioxidant protection decreased, while the content of TBA-positive products and lipid hydroperoxides (LOOHs) increased in liver tissues of animals with EDM from group II. The activity of CAT, glutathione peroxidase (GPx), glutathione reductase (GR) and the content of LOOHs and TBApositive products increased in kidney tissue homogenates in rats from group II. The content of reduced glutathione (GSH) and the activity of SOD decreased in the kidneys of animals with EDM compared to control. Pro/antioxidant status of the liver and kidneys was stabilized for the introduction of animal vanadium citrate. Indicators approached the level of control animals. The activity of GPx and the content of LOOHs decreased, the content of GSH increased in liver and kidney tissues. The activity of CAT and GR increased in liver tissues, and the indices had the opposite character in the tissues of the kidneys. Conclusion. Obviously, vanadium, like insulin-mimetic and antioxidant, has the ability to accept free radicals and, accordingly, reduce oxidative stress in tissues of diabetic animals.
diabetes mellitus, vanadium citrate, antioxidant enzymes, alloxan, rats.
- Shaw JE, Sicree RA, Zimmet PZ. Global estimates of the prevalence of diabetes for 2010 and 2030. Diabetes Research and Clinical Practice. 2010;87:4-14.
- Jakuscha T, Kissa T. In vitro study of the antidiabetic behavior of vanadium compounds. Coordination Chemistry Reviews. 2017;351:118-26.
- Thompson KH, Lichter J, LeBel C, Scaife MC, McNeill JH, Orvig C. Vanadium treatment of type 2 diabetes: a view to the future. Journal of Inorganic Biochemistry. 2009;103:554-8.
- Shradha B, Sisodia SS. Diabetes, dyslipidemia, antioxidant and status of oxodaton stress. International Journal of Research in Ayurveda and Pharmacy. 2010;1:33-42.
- Sheikhpour R. Incretin, dipeptid peptidase 4 and inhibitors and diabetes. Lambert. 2012;1-13.
- Lowry OH, Rosenbrough NJ, Farr AL, Randall R. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry. 1951;193(1):265-275.
- Mironchik VV. Sposob opredeleniya gidroperekisey lipidov v biologicheskikh tkanyakh. Avtorskoye svidetel’stvo. 1998; № 1084681 SSSR, MKI G №33/48. (SSSR). № 3468369/2813; Byul. № 13. [in Russian].
- Korobeynikova SN. Modifikacija opredelenija produktov POL v reakcii s tiobarbiturovoj kislotoj. Laboratornoye delo. 1989;7:8-9. [in Russian].
- Chevari S, Andyal T, Shtrenger Ya. Opredelenie antioksidantnyih parametrov krovi i ih diagnosticheskoe znachenie v pozhilom vozraste. Laboratornoe delo. 1991;10:9-13. [in Russian].
- Moin VM. Prostoj i specificheskij metod opredelenija aktivnosti glutationperoksidazy v jeritrocitah. Laboratornoye delo. 1986;12:724-7. [in Russian].
- Korolyuk MA, Ivanova MI, Maiorova IT, Tokarev VE. Metod opredelenija aktivnosti katalazy. Laboratornoye delo. 1988;1:16-9. [in Russian].
- Carlberg I, Mannervik B. Glutathione reductase. In Methods in enzymology. 1985;113:484-90.
- Batler O, Dyubra O. Metodika opredeleniya urovnya vostanovlenogo glutationa (GSN) v eritrotsitah krovi (po printsipu Batler, O. Dyubon, B. Kelli, 1963): metodicheskie rekomendatsii po differentsialnoy diagnostike razlichnyih form ishemicheskoy bolezni serdtsa s ispolzovaniem opredeleniya komponentov glutationovoy protivoperekisnoy kataliticheskoy sistemyi v eritrotsitah krovi. Odesa; 1982. s. 16-20. [in Russian].
- Li M, Smee JJ, Ding W, Crans DC. Anti-diabetic effects of sodium 4-amino-2,6-dipicolinatodioxovanadium(V) dihydrate in streptozotocininduced diabetic rats. Journal of Inorganic Biochemistry. 2009;103(4):585-9.
- Rohilla A, Ali S. Alloxan Induced Diabetes: Mechanisms and Effects. International Journal of Research in Pharmaceutical and Biomedical Sciences. 2012;3:819-23.
- Tunali S, Yanardag R. Protective effect of vanadyl sulfate on skin injury in streptozotocin-induced diabetic rats. Human & Experimental Toxicology. 2013;32(11):1206-12.
- Shatynska O, Iskra R. Correction magnesium citrate oxidative stress in blood of rats with experimental diabetes. Biology. 2016;1(71):81-3.
Publication of the article:
«Bulletin of problems biology and medicine» Issue 1 Part 2 (149), 2019 year, 75-79 pages, index UDK 546.881:678.048