COMPARATIVE IMMUNOHISTOCHEMICAL ANALYSIS OF THE LOCAL IMMUNE RESPONSE DURING THE EXACERBATION STAGE OF COPD AND ACUTE PHLEGMONOUS APPENDICITIS

Kuyun L. O.

CLINICAL AND EXPERIMENTAL MEDICINE

Scentific article

Objective: use immunohistochemical approach to analyze the immunocompetent cell composition of terminal bronchioles in patients with chronic obstructive pulmonary diseases (COPD) and immunocompetent cells of the lamina propria of the appendix in patients with acute phlegmonous appendicitis. Object and methods. Histological samples originated from 10 patients who had been operated on account of bullous emphysema (COPD) and 21 patients with acute phlegmonous appendicitis. The control group consisted of 6 patients who did not have COPD and 15 patients who did not have inflammation of the abdominal cavity. Lung tissue and the lamina propria tissue samples were taken accordingly from both groups and submitted for sectorbased cross section analysis. Tissue samples of the lung and lamina propria were placed into 10% neutral-buffered formalin for 18-24 hours. After that, tissue pieces were extracted. 3 slices 3-5 mm long were obtained by cross section of the lung tissue. The samples were dehydrated using ethanol solutions of different concentrations and dipped in paraffin wax. Serial cross-section samples 4 mkm in width were produced using a rotatary microtome (Leica RM 2125 RT, Germany). The samples were placed on slides and colored using hematoxylin and eosinum (Merck, Germany) and on the SuperFrost Plus adhesive slides for further immunohistological research. Immunohistochemical (IHC) studies were performed by using primary monoclonal antibodies of mouse to Ki-67 (proliferation index), CD3, CD4, CD8, CD20, CD45, CD45R0 and CD68 (DAKO, Denmark) and EnVision + System-HRP imaging systems (DAB). The data obtained in the experiment was statistically processed using “Minitab 16” statistical software. Results and their discussion. The results of the study established that, in acute phlegmonous appendicitis, unlike COPD, a local immune response is realized by infiltration of the lamina propria with macrophages, Ki-67 + cells, B-lymphocytes and memory T-cells. The similarity of inflammation in COPD and phlegmonous appendicitis is characterized by increased migration of T-lymphocytes (CD3 +), T-helpers (CD4 +) and cytotoxic T-lymphocytes (CD8 +) into the lamina propria, although with greater intensity for COPD. Significance of the research. The importance of the application of this data based both on quantitative and qualitative characteristics of the local response of CD4+ and CD8+ T – lymphocyte subpopulations is undeniable. On the one hand, these subpopulations determine the course that the disease will take in COPD patients and aid in developing the most efficient and specific immune therapy. On the other hand, acute local inflammation of the lamina propria in the appendix is associated with increased infiltration of the mucous membrane by B-lymphocytes, macrophages, and memory T-cells, as well as active proliferation of mononuclear cells. The author is convinced that individual approach to treatment of COPD patients and patients with peritonitis is vital. These findings serve as helpful diagnostic criteria for COPD and acute phlegmonous appendicitis accordingly.

COPD, cellular infiltration of bronchioles, immunohistochemistry, local immunity

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«Bulletin of problems biology and medicine» Issue 2 (144), 2018 year, 176-180 pages, index UDK 616.24-007.271-036.12+616.346.2-002.3]-097

10.29254/2077-4214-2018-2-144-176-180