Prokopiuk V. Yu., Falko O. V., Karpenko V. G., Chub O. V., Loginova O. O.


About the author:

Prokopiuk V. Yu., Falko O. V., Karpenko V. G., Chub O. V., Loginova O. O.



Type of article:

Scentific article


Autoimmune diseases affect about 3-5% of people. The mesenchymal stem cells and placental bioobjects application is a promising, non-toxic method of treatment. The mechanism of the influence of native and cryopreserved placental derivatives on immunocompetent cells remains unclear. The aim of the work was to compare the effect of freshly isolated and cryopreserved placental derivatives on splenocytes. Object and methods. Mouse splenocytes were cultured in media conditioned with native and cryopreserved placental cells and explants, with a medium containing 10% of the placental extract. The metabolic activity of splenocytes was assessed by MTT test, functional activity was assessed by the blasttransformation reaction. Results. It has been shown that the cells conditioned by placental cells, explants, and placenta extract reduce the metabolic activity of splenocytes and the activity of the blasttransformation reaction. The media, conditioned by cryopreserved placental cells and explants, reduce the metabolic activity of splenocytes in a bigger extent, than those that are conditioned with freshly isolated objects. Conclusions. Various placental derivatives application is promising treatment of autoimmune diseases, but not acute infectious diseases.


splenocytes, culture, placenta, cells, extract, cryopreservation


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Publication of the article:

«Bulletin of problems biology and medicine» Issue 2 (144), 2018 year, 221-224 pages, index UDK 615.361. 57.085.23