PECULIARITIES OF THE CONDITIONAL PATHOGENIC MICROFLORA OF UROGENITAL TRACT IN DYSBIOTIC DISORDERS

Kostyria O. V., Golodok L. P., Skliar T. V.

MICROBIOLOGY

Scentific article

The 90 pathologists of pathogens of the urogenital tract isolated and identified in 90 women aged 20-70 years in the Diagnostic Center of the Medical Academy of the city of Dnipro using the polymerase chain reaction method. In women of reproductive age, in the normocenosis of the microbiota is predominantly lactobacilli (Lactobacillus spp.), but in dysbiotic disturbances, the microbial composition of urogenital tract (UGT) is characterized by a decrease in the amount of lactobacilli and the substitution of them by conventionally pathogenic anaerobic microorganisms. The imbalance of the UHT female biota is a violation of the quantitative and qualitative relationships of resident microorganisms (saprophytic and conditionally pathogenic) that inhabit the genitourinary system in norm. For violations of UHT microflora in high concentrations, representatives of the genera Gardnerella vaginallis, Prevotella, Veilonella spp., Mobiluns spp., Peptostreptococcus spp., Atopobium vaginallis, Candida spp. Such violations can lead to bacterial vaginosis (BV) – 38%, aerobic vaginitis – 20%, candidiasis – 10%, association of BV with other dysbiotic disorders – 16%, other complications – 16%. We have found that bacterial vaginosis is more common in patients of reproductive age. In women of this age category, Gardnerella vaginallis, Atopobium vaginallis, Veilonella spp. are allocated at 15-20% higher concentrations than other age groups. Classical bacterial vaginosis, caused by one pathogens – G. vaginalis, occurs only in 26% of cases. In 21% of patients BV was combined with urogenital candidiasis. Other 53% of BV patients are found in combination with myco- and ureaplasma, yeast-like fungi of the genus Candida, facultative-anaerobic and aerobic opportunistic pathogens). Number of Lactobacillus spp. changed from 108 to 105 CFU/ml. Representatives of the genera Gardnerella vaginallis, Prevotella, Veilonella spp., Mobiluns spp., Peptostreptococcus spp., Atopobium vaginallis tended to increase their number from 103-104 to 106-107 CFU/ml. In aerobic vaginitis, there is a decrease in Lactobacillus spp. from 108 to 106 CFU/ml. Representatives of the genera Enterobacteriaceae spp., Streptococcus spp., Staphylococcus spp. increased from 103 to 105 CFU/ml. At the same time, the number of other conditionally pathogenic microorganisms almost did not change and remained at 103-104 CFU/ml. It was also found that among women aged 40-55 years with dysbiotic disturbances, yeast-like fungi of the genus Candida are 20% more likely to be found in women of reproductive age and account for 40% of cases.

PCR in real time, dysbiosis, urogenital tract, child-bearing potential

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«Bulletin of problems biology and medicine» Issue 2 (144), 2018 year, 293-297 pages, index UDK 579.61:616-078

10.29254/2077-4214-2018-2-144-293-297