IMPACT OF ACUTE TOTAL-BODY GAMMA-IRRADIATION ON SPERM PRODUCTION OF LABORATORY RATS

Nykolaichuk R. P., Klepko A. V

CLINICAL AND EXPERIMENTAL MEDICINE

Scentific article

Male gonads are among the most sensitive organs to different xenobiotics, especially to ionizing radiation. After two, the biggest in the world, nuclear disasters in Chornobyl (1986) and Fukushima (2011) for the last few years many researches began to study effect of low-dose IR on sperm parameters. Unfortunately, till this time, data are controversy and need deep study of this problem. Aim of this study was to study and to analyse impact of total single exposure to radiation (dose dependent) on parameters of sperm production of rats. Studying of total X-irradiation on laboratory rats of male sex has been performed. Rats were X-irradiated by five doses 0.6; 1.0; 2.0; 3.0 and 5.0 Gy, respectively, with dose rate 106,6 сGy/min. The animals were decapitated 5, 15, 30 and 65 days after total irradiation and sperm production parameters were analyzed. It has been established that sensitivity of testicles and epididymis to irradiation in rats is dose dependent. After irradiation with doses of 0.6-1.0 Gy from the 30 days of experiment was observed increasing of studied indicators that can be explained as hormesis effect. Total single irradiation of rats with doses of 2.0-5.0 Gy caused decreasing of sperm production and a significant decrease of viable spermatozoa.

total irradiation, epididymis, testicles, spermatogenesis, spermatozoa.

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«Bulletin of problems biology and medicine» Issue 2 (156), 2020 year, 133-137 pages, index UDK 54-78:[591.463.1:599.323.4]

10.29254/2077-4214-2020-2-156-133-137