CORRECTION OF CHRONIC PROSTATITIS BY A COMPOSITION OF NANOPARTICLES OF ORTHOVANADATES OF RARE-EARTH ELEMENTS WITH ADSORPED ZINC IONS

Chistyakova E. Ye., Smolenko N. P., Korenyeva Ye. M., Velychko N. F., Belkina I. O., Klochkov V. K.

CLINICAL AND EXPERIMENTAL MEDICINE

Scentific article

Possibilities of application of nanoparticles composition of rare earth elements with adsorbed zinc ions for correction of experimental chronic prostatitis effects in male rats were investigated. Male rats modeled abacterial inflammation of the prostate by cryotraumatism of the ventral part using a Cryo Wartner device (manufactured by Omega Pharma International, Belgium). GdVO4 :Eu3+ size 8-10 – 25 nm in a dose of 0.3 mg/kg with Zn2+ adsorbed to the orthotoxic matrix in a dose of 2·10-6 mole in an amount of 0.5 ml per animal (group CP+NP+Zn) and a reference preparation (0.5 % Prostatilen, in a volume of 0.5 ml per animal) (group CP+prostatylen) were injected into the upper rectum with a special catheter from the 15th day after surgery for 2 weeks. Control was provided by unordered males (Control group) and those that modeled a chronic prostatitis (group CP). In dynamics, leukocytosis was determined. Studied the state of spermatogenesis, determined the mass of the genital organs. Results. The use of NP and Prostatylene for the correction of the effects of CP resulted in a decrease in the number of leukocytes by 27 and 21 %, respectively, compared with the data of males of the CP group, which may indicate a decrease in the overall inflammatory response. The development of CP in rats in groups of CP, CP+NP+Zn, CP+prostatilen was accompanied by an increase in the mass ratios of the ventral part of the prostate (by 30, 18 and 20 %, respectively) and familial vesicles (by 27, 32 and 35 %, respectively; p<0.05) compared to similar controls. Analyzing the spermograms of animals, it was found that the total concentration of sperm and their mobility in males of the CP group significantly decreased (by 41 and 38 %, respectively; p<0.05) against the background of the growth of pathological forms (2.7 times), which eventually caused a decrease in the concentration of morphologically normal sperm (45 %, p<0.05). The use of zinc NP resulted in the restoration of the percentage of motile sperm to the size of control animals, that is, normalized their motility. Animals treated with prostatilene on the background of CP had similar changes in sperm counts, but there were some differences, in particular, the number of pathological gametes in animals of this group decreased by 43 % (p<0.05) compared with that of males in the CP group. Conclusions. The experimental data obtained indicate that the use of the GdVO4: Eu3 + LF composition with zinc ions adsorbed to the orthotoxic matrix has the ability to inhibit the development of a systemic inflammatory process and to reduce the effects of prostate cryotherapy

nanoparticles of rare earth elements with zinc ions, chronic prostatitis, leukocytes, spermogram, male rats.

1. Gladkova AI, Korobov AM. Terapiya lazernym izlucheniyem muzhskikh reproduktivnykh rasstroystv. Fiziologicheskiye i patogeneticheskiye aspekty. Khar’kov: Fakt; 2017. 208 s. [in Russian].
2. Korenêva YeM, Karpenko NO, Smolênko NP, Byelkina IO, Chystyakova EYe, Selyukova NYu, ta іn. Vplyv nanochastynok ortovanadatu gadoliniyu ta dioksydu tseriyu na spermohramu doroslykh samtsiv shchuriv iz neonatalʹno indukovanymy rozladamy reproduktyvnoyi funktsiyi. Probl. endokrin. patologіі. 2016;1:48-56. [in Ukrainian].
3. Karpenko NO, Korenieva YeM, Chystiakova EYe, Smolienko NP, Bielkina IO, Seliukova NYu, et al. Тhe influence of the rare-earth metals nanoparticles on the rat’s males reprductive function in the descending stage of ontogenesis. Ukr. Biopharm. J. 2016;4(45):75-80.
4. Selyukova NYu, Koreneva YеM. Vplyv sulʹfatu tsynku na seksualʹnistʹ i spermohramu statevozrilykh samtsiv shchuriv. Svit medytsyny ta biolohiyi. 2017;2(60):150-3. [in Ukrainian].
5.  Dissanayake AB, Wijesinghe PS, Ratnasooriya WD, Wimalasena S. Effects of zinc supplementation on sexual behavior of male rats. J. Hum. Reprod. Sci. 2009;2(2):57-61.
6. Gorpinchenko II, Gurzhenko YuN, Spiridonenko VV. Optimizatsiya pokazateley sekreta predstatel’noy zhelezy i eyakulyata pri muzhskom besplodii na fone khronicheskogo prostatovezikulita. Zdorov’ye muzhchiny. 2014;3:68-74. [in Russian].
7. Rakevich MV. Metody konservativnogo lecheniya muzhskogo besplodiya. Meditsinskiye novosti. 2016;9:13-7. [in Russian].
8. Omu AE, Al-Azemi MK, Kehinde EO. Indications of the mechanisms involved in improved sperm parameters by zinc therapy. Med Princ Pract. 2008;17(2):108-16.
9. Goodarzi D, Cyrus A, Baghinia MR. The efficacy of zinc for treatment of chronic prostatitis. Acta Med. Indones. 2013;45(4):259-64.
10. Molochkov VA. Sildenafil i chronicheskij prostatit. Al′manach klinicheskoj medicini. 2014;34:108-11. [in Russian].
11. Zaychenko GV, Andríyanenkov OV, Soldatova SO, Gevoyan SR. Noví pídkhodi do modelyuvannya kríotravmi peredmíkhurovoí̈ zalozi v yeksperimentí. Ínformatsíyniy list pro novovvedennya v sferí okhoroni zdorov’ya. К.: 2013. 28 s. [in Ukrainian].
12. Zhukov OB, Yevdokimov VV, Bragina YeYe. Uluchsheniye kachestva zhizni i morfofunktsional’nykh kharakteristik spermatozoidov u muzhchin s khronicheskim abakterial’nym prostatitomi programmy pregravidarnoy podgotovki k ottsovstvu. Andrologiya i genital’naya khirurgiya. 2017;18(1):3-9. [in Russian].
13. Zaytsev VI. Zastosuvannya transrektalʹnoho vvedennya preparativ pry likuvanni zakhvoryuvanʹ peredmikhurovoyi zalozy – perevahy ta nedoliky. Zdorov’ya cholovika. 2013;1:110-2. [in Ukrainian].
14. Petryshchev VS, Shchilynky AM. Otsinka morfolohiyi spermatozoyidiv z·hidno suvorym kryteriyam (ohlyad literatury). Problemy reproduktsiyi. 2002;3:87-91. [in Ukrainian].
15. Karpenko NO, Talko VV, Omelchuk ST, Lapta SS. Integral assessment of the reproductive function of male laboratory animals. Ukr Biopharm Zh. 2011;13(2):64-8.
16. Zagal’ni etichni printsipi eksperimentiv na tvarinakh. Endokrinologiya. 2003;8(1):142-5. [in Ukrainian].
17. Hlants SA. Mediko-biologicheskaya statistika. M.: Praktyka; 1998. 459 s. [in Russian].
18. Topka YeG, Baybakov VM, Rusín AV. Znachennya morfounktsíonal’nikh osoblivostey drenazhnikh sistem yaêchka ta sím’yanikh pukhirtsív u rozvitku prostatovezikulítu. Medichní perspektivi. 2011;16(4):15-20. [in Ukrainian].
19. Karpenko NA, Malukin YuV, Koreneva EM, Klochkov VK, Kavok NS, Smolenko NP, et al. The effects of chronic intake of nanoparticles of cerium dioxide or gadolinium ortovanadate into aging male rats. Nanomaterials: Applications & Properties: Proc. 3-rd Int. Conf., Alushta, Sept. 16-21, 2013. Alushta. 2013;2(4):04NABM28-1–04NABM28-4.
20. Hamdi SA, Nassif OI, Ardawi MS. Effect of marginal or severe zinc deficiency on testicular development and functions of the rat. Arch. Androl. 1997;38:243-53.

«Bulletin of problems biology and medicine» Issue 2 (156), 2020 year, 184-187 pages, index UDK 616.65-002:546.655:616-08

10.29254/2077-4214-2020-2-156-184-187