THE USE OF ANTISEPTICS FOR SIMULATION OF DYSBIOTIC DISORDERS IN THE EXPERIMENT

Bobyr V. V., Nazarchuk O. A.

MICROBIOLOGY

Scentific article

Modeling of dysbiotic disorders in the intestine is important for studying changes in the composition, properties of the intestinal microflora, and investigation of rational ways of correction of microecological disorders. Experimental dysbiosis in animals is nowadays often formed using antibacterial agents but the use of antiseptics is fundamentally new one. The aim was to investigate the possibility of using antiseptic drugs to model dysbiotic disorders in an experiment. The experiment was conducted on 45 mice (15 in each group), which were kept in accordance with the current “Sanitary rules for the organization, equipment and maintenance of experimental biological clinics (vivarium)”, on a standard diet consisting of granulated compound feed for laboratory animals. Access to water was unlimited. The control group used ordinary tap water. Each of the 325×215×85 mm polycarbonate cells with galvanized steel lids and glass water bowls contained 15 animals each. All work with animals was carried out in accordance with the Law of Ukraine “On the Protection of Animals from Cruelty and in accordance with the Ethical Rules and Regulations of Laboratory Animals” of 21.02.2006 No. 3447-IV, “European Convention for the Protection of Vertebrate Animals used for experimental and other scientific purposes” and “Council Directive 2010/63/EU of 22 September 2010 on the protection of animals used for scientific purposes”. The method of formation of dysbiotic conditions was as follows: using a tuberculin syringe with needle with thickening on the end of the animals orally administered decamethoxine (DCM) in a volume of 100 μl per animal per day (the first group – 0.01 mg, the second group – 0.02 mg). At the same time, a DCM solution of 0.02% was added to the water bowl. After 24 hours, repeated collection of faeces for quantitative and qualitative determination of microflora. The introduction of the DCM antiseptic solution and material selection was repeated for 5 days, followed by determination of the total amount of fecal microflora and its individual representatives daily and 2 days after discontinuation of the drug. Antibiotics (ampicillin and metronidazole) were administered to the animals 10 mg intramuscularly per animal per day. Normal microbial faeces composition was established in control animals. Thus, the total number of microorganisms was 3.2×108 CFU/g (E. coli – 2.2×104 CFU/g; Lactobacillus spp. – 1.4×107 CFU/g), Bifidobacterium spp. – 1,8×108 CFU/g). Bacteriological studies of faeces revealed the absence of pronounced dysbiotic disorders in animals using DCM. According to the markers of dysbiosis (total number of microorganisms, Lactobacillus spp., Bifidobacterium spp., E. coli), a weak effect of DCM antiseptic on microbiological parameters of the intestine has been shown. After 5 days of use of DCM, with increasing its concentration of signifficant dysbiotic disorders also was not registered. There was determined reduction of the total number of microorganisms (E. coli, Lactobacillus spp., Bifidobacterium spp.) to 1.4×106 CFU/g. The decrease in the total microbial number was not accompanied by a violation of the quantitative parameters of Lactobacillus spp. and Bifidobacterium spp. With the introduction of DCM (0.2 mg) in the drinking bowl dysbiotic disorders were observed in mice receiving antibiotics, that indicated the potentiation of activity of the second ones in the presence of antiseptic. After a five-day course and two days after discontinuation of the combination of antibiotics and antiseptics, the total microbial count was reduced to (5.4±0.2) × 103 CFU/g. Thus, the only use of DCM does not lead to a signifficant violation of the total number of microorganisms, the number of Lactobacillus spp., Bifidobacterium spp., E. coli, which in turn indicates a low risk of dysbiotic disorders when using this antiseptic.с

antibiotics, antiseptics, decamethoxin, dysbiosis.

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«Bulletin of problems biology and medicine» Issue 2 (156), 2020 year, 223-226 pages, index UDK 579:57.084.1

10.29254/2077-4214-2020-2-156-223-226