MONITORING OF DYSBIOTIC DISORDERS IN THE UROGENITAL TRACT OF WOMEN AND MEN OF DIFFERENT AGE CATEGORIES

Vinogradova K. O., Gavrilyuk V. G., Sklyar T. V., Sokolova I. E.

MICROBIOLOGY

Scentific article

In the present-day conditions, urogenital imbalance is an urgent problem in scientific and medical practice, given that not all mechanisms of development of dysbiotic conditions have been fully understood, there are no clear criteria for diagnosis, difficulties in the selection of effective therapy due to antibiotic resistance. Asymptomatic media and lack of a specific inflammatory pattern present difficulties in diagnosis, which in turn contributes to chronicity of the process, adverse effects on reproductive function and reduced quality of life. The method of polymerase chain reaction in real time makes it possible to identify pathogens even at their low concentration, to carry out etiological diagnostics in the early stages, to evaluate the qualitative and quantitative content of urogenital biocenosis, to control the quality of bioassay and the effectiveness of therapy. As a result of the PCR analysis in the real time composition of the microbiome of the urogenital tract among 474 patients of the Medical Diagnostic Center (Dnipro) during 2017-2019, in 290 persons there were found dysbiotic disorders. During the studied period, the tendency to decrease the incidence of dysbiotic conditions in the genital area in the persons of the youngest reproductive age and the elderly category is shown, but the growth of imbalances in women and men of the reproductive age of the older group (36-55 years) were present. Dysbiotic disorders were caused by a decrease in the bacterial titers of Lactobacillus bacteria to 0-106 CFU/ml and excessive colonization by opportunistic and pathogenic microorganisms: in the female genital area – Gardnerella vaginalis, Eubacterium spp., Ureaplasma spp. (106 -109 CFU/ml) in 33.33-74.55%, Candida spp. (104 -109 CFU/ml) in 20.0-30.0% of patients; in the urogenital system of men – Eubacterium spp., Bacteroides spp., Peptostreptococcus spp., Anaerococcus spp. (104 - 109 CFU/ml) in 58.33-100% of patients; and the presence of true pathogens – Mycoplasma genitalium, Trichomonas vaginalis, Neisseria gonorrhoae, Chlamydia trachomatis (103 -107 CFU/ml) in 1.66-9.31% of males. The results obtained from the basis are useful for finding and developing new diagnostic approaches, remedies and therapy of dysbiotic syndromes for the purpose of health for the next generations.

dysbiotic syndrome, urogenital tract, polymerase chain reaction, monitoring, age categories.

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«Bulletin of problems biology and medicine» Issue 2 (156), 2020 year, 226-231 pages, index UDK 579.61

10.29254/2077-4214-2020-2-156-226-231