APPLICATION OF VECTOR – PARAMETRIC FLUORESCENT POLARIZATION MICROSCOPY BLOOD FILMS OF RAT FOR DIFFERENTIAL DIAGNOSIS OF ABDOMINAL SEPSIS

Solovey Yu. M, Polovyy V. P, Solovey M. M, Velushchak I. D.

CLINICAL AND EXPERIMENTAL MEDICINE

Scentific article

Abstract. In the experimental conditions on 273 nonlinear white rats simulated abdominal sepsis of varying severity: mild, moderate and severe. The control group was intact animals. Blood samples were taken at 12, 48, and 78 hours of the experiment. Prepared films of blood on slides. Irradiation with a parallel beam of a blue laser was performed. The structure of the polycrystalline component of biological preparations of rats’ blood in the differential diagnosis of the severity of the septic condition was investigated by digital Stokes-polarimetric fluorescence microscopy. An album of vector-parametric polarization maps and distributions of the number of characteristic values of the crystallization parameter at the points of digital fluorescent microscopic imaging of polycrystalline blood films of rats from control group 1 and experimental groups 2 – 4 with different severity of septic pathology was obtained. The statistical significance of differentiation of vector-parametric maps of fluorescent microscopic imaging of polycrystalline blood films of rats from control group 1 and experimental groups 2 – 4 with different severity of septic pathology was determined. The most diagnostically sensitive statistical criteria for differentiation of vectorparametric polarization maps of fluorescent microscopic imaging of polycrystalline films of blood of rats from control group 1 and experimental groups 2 – 4 with different severity of septic pathology were found. The operational characteristics of the diagnostic power of the method of Stokes-polarimetric microscopy of polycrystalline films of blood of rats of control and experimental groups are determined. Balanced accuracy was established: differentiation of healthy and sick sepsis rats, intergroup differentiation of the severity of the septic process of sick rats; intragroup differentiation of patients with sepsis in rats.

abdominal sepsis, polarimetry, Stokes vector.

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«Bulletin of problems biology and medicine» Issue 2 (160), 2021 year, 126-131 pages, index UDK 616.381-002.3:616.94]-036.1-079.4:616.15-073.524:535.37

10.29254/2077-4214-2021-2-160-126-131