CHARACTERISTICS OF CHANGES IN THE STRUCTURAL COMPONENTS OF THE MUCOSA AND CARTILAGE OF THE LARYNX OF RATS AT THE END OF THE SECOND WEEK OF THE EXPERIMENTAL OPIOID EFFECT

Ivasivka K. P, Paltov E. V, Masna Z. Z.

MORPHOLOGY

Scentific article

Absract. In our work we aimed to study the pathomorphological manifestations among the structural components of the laryngeal mucosa and cartilage in the early stages of the experimental opioid effect. This goal was achieved through the use of histological techniques to visualize the structural components of the laryngeal mucosa and cartilage. Histological sections with a thickness of 5-7 mm were made. Histological specimens were prepared according to conventional methods using the dye hematoxylin, eosin, azan according to the method of Heidenhain, PAS reaction according to Mac-Manus and Alcian blue according to Steedman. Microscopic examinations and photographing of the preparations were performed using an MBI-1 microscope and a Nicon D 3100 digital camera. At the microstructural level, after fourteen days in rats, an increase in the permeability of the vascular wall was detected, as a result of which erythrocytes accumulated on the surface of the mucous membrane of different parts of the larynx. Also noted moderate polyphonocellular infiltration of the connective tissue of the lamina propria of the epiglottis, more pronounced near the laryngeal surfac. Hyperemia, stasis, perivascular edema, as well as polymorphonuclear infiltration of the main connective tissue substance developed in the vessels of the own plate of the subchondral mucosa. Erythrocytes accumulated on the surface of the subchondral mucosa. The results of the study in the future will form a pathomorphological basis that can be used for comparative characterization of the dynamics of growth of pathomorphological changes in the structural components of the laryngeal mucosa and cartilage in the early stages and compare these changes with pathomorphological manifestations in the late stages of experimental opioid effects. . The above will provide an opportunity to establish the most optimal time periods for which there is a characteristic set of pathomorphological manifestations of the initial phenomena in the structural components of the laryngeal mucosa and cartilage and to trace the dynamics of these changes in the long term experimental opioid effects.

opioid, laryngeal mucosa, laryngeal cartilage, rat, early terms.

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«Bulletin of problems biology and medicine» Issue 2 (160), 2021 year, 217-223 pages, index UDK 611.321.018.3+611.321.018.73.019):615.212.7

10.29254/2077-4214-2021-2-160-217-223